Plasmids and Bacteria

From 2006.igem.org

• pSB1A3
  • • amp resistant
    • copy number:100-300
    • size:2157 empty

• pSB1AC3
  • • part-BBAp1000
    • copy number:100-3000
    • amp and CmR resistant
    • contains device that provides CmR
    • size:3844 ( with device) 3005 (without device)

• pSB1AK3
  • • part BBa_|13535
    • copy number:100-300
    • amp and kan resistance
    • contains a device for screening test
    • size: 3457 (with device) 3189 (without device)

• Transform bacteria We are using Salmonella typhimurium for the Hin protein as it was the referenced species in all of the literature.
• Create RE biobrick (completed)

• Isolating genomic DNA from Salmonella typhimurium
• Ordered oligos for recombinational enhancer and primers for the hin gene