Flip One Pancake

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Flipping One Pancake

The plan is to construct a plasmid that contains the following:

  1. Amp resistance gene as in pSB1A3
  2. Hin recombinase gene driven by lacP (inducible in JM109 by IPTG or lactose), cloned from PCR product by Davidson team
  3. RE (recombination enhancer), synthesized by Davidson team
  4. One Pancake test construct: 
      a. araBAD promoter, inducible with arabinose
      b. RBS
      c. hixL recombination site, synthesized by Missouri Western team
      d. CDS for mRFP in forward direction
      e. hixR recombination site, synthesized by Missouri Western team
      f. double forward terminator
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