Hin gene: Part BBa J31000

From 2006.igem.org

Hin gene: Part BBa_J31000[http://partsregistry.org/Part:BBa_J31000]

Primers for Hin gene

• The 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in pink.

Forward primer

• Consists of four extra base pairs, XBA restriction site, and the first 85 base pairs of the [http://partsregistry.org/Part:BBa_J31000| Hin gene]

5’ GCATTCTAGAATGGCTACTATTGGGTATATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTACCAGTGCAAATTG 3’

Reverse primer

• Consists of four extra base pairs, Bio Brick, the last 23 base pairs of the Hin gene

5’ ATGCCTGCAGGCGGCCGCACTAGTTTAATTCATTCGTTTTTTTATAC 3’