Answer T1
From 2006.igem.org
For in vitro recombination, < 10 mM Mg++ and 30% glycerol traps reaction after DNA cleavage and 2 uM EtBr slows strand exchange.
Unclear whether these conditions could be established in E coli.
In Rippe et al. 1997, superhelical density of a 1868 bp plasmid was -0.03, with 80% of plasmids having -6 supercoils.
