Answers to this one and the next

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R.C. Johnson and M.F. Bruist. 1989. Intermediates in Hin-mediated DNA inversion: a role for Fis and the recombinational enhancer in the strand exchange reaction
R.C. Johnson and M.F. Bruist. 1989. Intermediates in Hin-mediated DNA inversion: a role for Fis and the recombinational enhancer in the strand exchange reaction
[http://www.pubmedcentral.gov/picrender.fcgi?tool=pmcentrez&blobtype=pdf&artid=400990]
[http://www.pubmedcentral.gov/picrender.fcgi?tool=pmcentrez&blobtype=pdf&artid=400990]
-
-RE sequence must be present
+
#RE sequence must be present
-
-w/o RE, Hin can still assemble to with the two Hix’s however, Hin can’t generate inversion
+
#w/o RE, Hin can still assemble to with the two Hix’s however, Hin can’t generate inversion
-
-Prevention of Fis binding causes loss of enhancer function
+
#Prevention of Fis binding causes loss of enhancer function
-
-60 bp in length
+
#60 bp in length
-
-Binds two Fis proteins (98 AA’s)
+
#Binds two Fis proteins (98 AA’s)
-
-Enhancer cannot function when located too close to a recombination site
+
#Enhancer cannot function when located too close to a recombination site
-
-HU facilitates the required bending of the DNA to create the inversion interaction
+
#HU facilitates the required bending of the DNA to create the inversion interaction
-
-HU stimulation is dependent upon location of RE
+
#HU stimulation is dependent upon location of RE
-
-Dependence upon HU is lesser when distance between RE and recombination site is greater
+
#Dependence upon HU is lesser when distance between RE and recombination site is greater
-
-Required to induce the bending of the shorter, yet stiffer, segment of DNA  
+
#Required to induce the bending of the shorter, yet stiffer, segment of DNA  
-
-When RE is located >650 bp for the recombination site the dependence upon HU to form the complex is lost
+
#When RE is located >650 bp for the recombination site the dependence upon HU to form the complex is lost
-
-RE combined w/ supercoiling mediate the rotation of DNA strands subsequent to double-stranded cleavage by Hin within two recombination sites
+
#RE combined w/ supercoiling mediate the rotation of DNA strands subsequent to double-stranded cleavage by Hin within two recombination sites
Haykinson and Johnson. 1993. DNA looping and the helical repeat in vitro and in vivo: effect of HU protein and enhancer location on Hin invertasome assembly
Haykinson and Johnson. 1993. DNA looping and the helical repeat in vitro and in vivo: effect of HU protein and enhancer location on Hin invertasome assembly
[http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=8508775]
[http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=8508775]
-
-The two recombination sites bound by Hin are assembled together at the Fis-bound RE
+
#The two recombination sites bound by Hin are assembled together at the Fis-bound RE
-
-w/o HU invertasome assembly is very inefficient
+
#w/o HU invertasome assembly is very inefficient
-
-“HU is the only protein in E. coli that can promote invertasome formation with  
+
#“HU is the only protein in E. coli that can promote invertasome formation with  
short DNA lengths between RE and recombination sites
short DNA lengths between RE and recombination sites
-
-HU binds non-specifically to DNA between the enhancer and recombination site to facilitate DNA looping
+
#HU binds non-specifically to DNA between the enhancer and recombination site to facilitate DNA looping
-
-“The RE can function at great distances !!! >4 kb !!! and on either side of a recombination site on a plasmid to stimulate Hin-mediated DNA inversion”
+
#“The RE can function at great distances !!! >4 kb !!! and on either side of a recombination site on a plasmid to stimulate Hin-mediated DNA inversion”
-
-RE is not functional at a position 32 bp minimum
+
#RE is not functional at a position 32 bp minimum
-
-Due to restricted looping
+
#Due to restricted looping

Revision as of 16:31, 30 May 2006

Recombinase Enhancer R.C. Johnson and M.F. Bruist. 1989. Intermediates in Hin-mediated DNA inversion: a role for Fis and the recombinational enhancer in the strand exchange reaction [1]

  1. RE sequence must be present
  2. w/o RE, Hin can still assemble to with the two Hix’s however, Hin can’t generate inversion
  3. Prevention of Fis binding causes loss of enhancer function
  4. 60 bp in length

#Binds two Fis proteins (98 AA’s)

  1. Enhancer cannot function when located too close to a recombination site
  2. HU facilitates the required bending of the DNA to create the inversion interaction
  3. HU stimulation is dependent upon location of RE
  4. Dependence upon HU is lesser when distance between RE and recombination site is greater

#Required to induce the bending of the shorter, yet stiffer, segment of DNA

  1. When RE is located >650 bp for the recombination site the dependence upon HU to form the complex is lost
  2. RE combined w/ supercoiling mediate the rotation of DNA strands subsequent to double-stranded cleavage by Hin within two recombination sites

Haykinson and Johnson. 1993. DNA looping and the helical repeat in vitro and in vivo: effect of HU protein and enhancer location on Hin invertasome assembly [2]

  1. The two recombination sites bound by Hin are assembled together at the Fis-bound RE
  2. w/o HU invertasome assembly is very inefficient
  3. “HU is the only protein in E. coli that can promote invertasome formation with

short DNA lengths between RE and recombination sites

  1. HU binds non-specifically to DNA between the enhancer and recombination site to facilitate DNA looping
  2. “The RE can function at great distances !!! >4 kb !!! and on either side of a recombination site on a plasmid to stimulate Hin-mediated DNA inversion”
  3. RE is not functional at a position 32 bp minimum

#Due to restricted looping

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