Anticipated Results & Significance
From 2006.igem.org
| Line 10: | Line 10: | ||
</center> | </center> | ||
| - | <h1> | + | <h1>Results and their significance for understanding the mechanism of immune response</h1> |
<p>Transfected cells have now TLR receptor (4 or 5) and our construct under NFκB promoter. We expect that after stimulation (with LPS or flagellin) cytoplasmic NFκB become active, migrate into nucleus and induce expression of our construct. Accumulating of dominant negative protein should block downstream signalling pathway. Concentration of active transcription factor should decrease and no more product should be synthesized. The rate of transcription and translation should be detected with detection systems - decrease of luminescence, free NFκB and::: (depends on detection system). In case that our construct is followed by PEST (degradation) sequence, it is expected that product should degrade. If stimulus is still present, NFκB will become active. Our product will be synthesized again. | <p>Transfected cells have now TLR receptor (4 or 5) and our construct under NFκB promoter. We expect that after stimulation (with LPS or flagellin) cytoplasmic NFκB become active, migrate into nucleus and induce expression of our construct. Accumulating of dominant negative protein should block downstream signalling pathway. Concentration of active transcription factor should decrease and no more product should be synthesized. The rate of transcription and translation should be detected with detection systems - decrease of luminescence, free NFκB and::: (depends on detection system). In case that our construct is followed by PEST (degradation) sequence, it is expected that product should degrade. If stimulus is still present, NFκB will become active. Our product will be synthesized again. | ||
Revision as of 10:49, 23 October 2006
| Home | Background and Signalling Pathway | Proposal & Approach | Troubleshooting, References & Sponsors | Team members |
|---|
Results and their significance for understanding the mechanism of immune response
Transfected cells have now TLR receptor (4 or 5) and our construct under NFκB promoter. We expect that after stimulation (with LPS or flagellin) cytoplasmic NFκB become active, migrate into nucleus and induce expression of our construct. Accumulating of dominant negative protein should block downstream signalling pathway. Concentration of active transcription factor should decrease and no more product should be synthesized. The rate of transcription and translation should be detected with detection systems - decrease of luminescence, free NFκB and::: (depends on detection system). In case that our construct is followed by PEST (degradation) sequence, it is expected that product should degrade. If stimulus is still present, NFκB will become active. Our product will be synthesized again.
Komentarji: Na osnovi modela smo si izbrali konstrukte bla bla bla in na podlagi tega pričakujemo da bi pokazali to in to....
| Home | Background and Signalling Pathway | Proposal & Approach | Troubleshooting, References & Sponsors | Team members |
|---|
