Berkeley2006-NetworksMain
From 2006.igem.org
Nodes are Built from Multiple Cell Types
Concentration in Culture Gives Graded Responses
Trainable Networks are Built from Nodes
Each cell has a complete lock-dependent communication system
Each cell has a unique lock address and a key message to send
Communication between a sender and a receiver is therefore restricted to cells that are a donor with the key that unlocks the recipient's lock address
There are many lock and key pairs, but the partnering between potential communicating cells is restricted in layers--layer 1 cells only contain keys that correspond to layer 2 locks. Layer 2 keys only unlock layer 3 locks, etc.
Within a layer, each n+1 layer lock component has a key with all permutations of layer n. If each layer has 10 addresses (locks) , there are 10x10, or 100 unique genotypes within each member of a layer is able to communicate with each member of the previous layer.
At the top of the layers is a set of training cells which direct a positive selectable marker to cells that contain their key sequence. The 'input' to the training system is therefore a set of key sequences.
At the end of the layers is a set of training cells that direct a negatively selectable marker, or "kill" signal contained in their key sequence. All cells able to receive the kill signal die.
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