Biobricks

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== Construction So Far ==
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= Biobricks Constructed By Us =
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{| border="1"
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|-
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| '''Biobrick''' || '''Construction''' || '''Ligations''' || '''Testing''' || '''Sequencing''' || '''Use'''
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|-
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| E. coli ArsR || Complete || To LacZ || Not Working || Not Yet || Detection of Arsenic
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|-
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| B. subtilis ArsR || Complete || To LacZ || Not Tested || Not Yet || Detection of Arsenic
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|-
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| LacZ || Complete || To ArsR and Terminator || Tests Underway || Not Yet || Lowering pH/Reporting
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|-
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| Bacillus Urease || Site specific mutagenesis required || To ligate to hybrid promoter || N/A || Not Yet || Raising pH
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|-
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| lambda cI/lacI Promoter || Underway || To ligate to urease || N/A || Not yet || Repressed by lambda cI and lacI in absence of lactose
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|-
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|}
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== Primers Etc. ==
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All biobricks have to have the following:
All biobricks have to have the following:
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(5’) <font color='red'> tactagtagcggccgctgcagcctt </font> (3’)
(5’) <font color='red'> tactagtagcggccgctgcagcctt </font> (3’)
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{| border="1"
 
-
|-
 
-
| Biobrick || Construction || Ligations || Testing || Sequencing || Use
 
-
|-
 
-
| E. coli ArsR || Complete || To LacZ || Not Working || Not Yet || Detection of Arsenic
 
-
|-
 
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| B. subtilis || Complete || To LacZ || Not Tested || Not Yet || Detection of Arsenic
 
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|}
 

Revision as of 14:14, 17 August 2006

Contents

Construction So Far

Biobricks Constructed By Us

Biobrick Construction Ligations Testing Sequencing Use
E. coli ArsR Complete To LacZ Not Working Not Yet Detection of Arsenic
B. subtilis ArsR Complete To LacZ Not Tested Not Yet Detection of Arsenic
LacZ Complete To ArsR and Terminator Tests Underway Not Yet Lowering pH/Reporting
Bacillus Urease Site specific mutagenesis required To ligate to hybrid promoter N/A Not Yet Raising pH
lambda cI/lacI Promoter Underway To ligate to urease N/A Not yet Repressed by lambda cI and lacI in absence of lactose


Primers Etc.

All biobricks have to have the following:


Prefix

(5’) cctttctagag (3’)


Suffix

(5’) tactagtagcggccgctgcagcctt (3’)


ArsR with Ribosome Binding Site Biobrick

Front primer sequence with Biobrick prefix

(5’) cctttctagag ccaactcaaaattcacac (3’)


Back primer sequence with Biobrick suffix

(5’) tactagtagcggccgctgcagcctt catatgtgtttagc (3’)


LacZ with Ribosome Binding Site Biobrick

Front primer sequence with Biobrick prefix

(5’) cctttctagag gaaacagctatgaccatg (3’)


Back primer sequence with Biobrick suffix

(5’) tactagtagcggccgctgcagcctt gaggggacgacgacag (3’)


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