Brown:Equipment needed

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       $ 400.00  Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>  custom primers   
       $ 400.00  Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>  custom primers   

Revision as of 21:45, 17 May 2006

Jason, I just threw this in here from the budget, please feel free to edit it out and add your equipment list, cheers, --Johncumbers 10:48, 17 May 2006 (EDT) john

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      $ 400.00   Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>   custom primers   
      $ 800.00   Antibodies for identifying cell division proteins 4 x $200   
      $ 250.00   disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates)   
      $ 600.00   Gel kit: Agarose gel aparatus, power source   
         TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros   
         Taq polymerase kit, DNTP - Free bases (actg ready mixed)   
      $ 600.00   Restriction enzymes  $60 x 10   
      $ 300.00   Thermocycler   
      $ 500.00   misc. equipment and supplies   
      $ 4,000.00   DNA synthesis costs $1.40 per base.   
        Protein coding regions are about 700 to 1000 bases long  This budget is for 3 - 4 new proteins.
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