Brown:Equipment needed

From 2006.igem.org

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
Jason, I just threw this in here from the budget, please feel free to edit it out and add your equipment list,
+
{| border=1 cellspacing=0 cellpadding=5
-
cheers,
+
|-
-
--[[User:Johncumbers|Johncumbers]] 10:48, 17 May 2006 (EDT) john
+
| '''Equipment'''
-
 
+
|
-
lists can be create by using the asterix symbol, or the pound symbol for numbered lists,
+
|-
-
+
| freezers/fridges
-
item 1<br>
+
|
-
item 2<br>
+
|
-
item 3<br>
+
|
 +
|-
 +
| incubator
 +
|-
 +
| hot bath
 +
|-
 +
| cold bath
 +
|-
 +
| autoclave
 +
|-
 +
| cell shaker/incubator
 +
|-
 +
| source of ddH2O
 +
|-
 +
| hot plates w/ stirring
 +
|-
 +
| stir bars (set)
 +
|-
 +
| PCR thermocycler
 +
|-
 +
| microcentrifuge (w/temp cntrl.)
 +
|-
 +
| centrifuge
 +
|-
 +
| Electrophoresis gel set
 +
|-
 +
| Gel Power source
 +
|-
 +
| UV Gel viewer
 +
|-
 +
| UV Gel camera
 +
|-
 +
| Pipettemen
 +
|
 +
|
 +
|
 +
|-
 +
| Electronic pipettor
 +
|-
 +
| bunsen burners
 +
|-
 +
| sparkers
 +
|-
 +
| wire loop
 +
|-
 +
| metal loop
 +
|
 +
|-
 +
| Glassware
 +
|
 +
|-
 +
| Bottles
 +
|
 +
|
 +
|
 +
|-
 +
| Beakers
 +
|
 +
|
 +
|-
 +
| Centrifuge tubes
 +
|
 +
|
 +
|-
 +
| Graduated cylinders
 +
|
 +
|
 +
|-
 +
| Electronic pipette tips (packages)
 +
|
 +
|
 +
|
 +
|
 +
|-
 +
| Gloves (boxes)
 +
|
 +
|
 +
|
 +
|
 +
|-
 +
| Plasticware
 +
|
 +
|-
 +
| centrifuge tubes
 +
|
 +
|
 +
|-
 +
| Pipette tips
 +
|
 +
|
 +
|
 +
|-
 +
| Bacterial cell culture plates
 +
|-
 +
| bacterial cell culture tubes
 +
|
 +
|-
 +
| tube racks/holders
 +
|
 +
|
 +
|
 +
|}
       $ 400.00  Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>  custom primers   
       $ 400.00  Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>  custom primers   

Revision as of 22:03, 17 May 2006

Equipment
freezers/fridges
incubator
hot bath
cold bath
autoclave
cell shaker/incubator
source of ddH2O
hot plates w/ stirring
stir bars (set)
PCR thermocycler
microcentrifuge (w/temp cntrl.)
centrifuge
Electrophoresis gel set
Gel Power source
UV Gel viewer
UV Gel camera
Pipettemen
Electronic pipettor
bunsen burners
sparkers
wire loop
metal loop
Glassware
Bottles
Beakers
Centrifuge tubes
Graduated cylinders
Electronic pipette tips (packages)
Gloves (boxes)
Plasticware
centrifuge tubes
Pipette tips
Bacterial cell culture plates
bacterial cell culture tubes
tube racks/holders
      $ 400.00   Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>   custom primers   
      $ 800.00   Antibodies for identifying cell division proteins 4 x $200   
      $ 250.00   disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates)   
      $ 600.00   Gel kit: Agarose gel aparatus, power source   
         TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros   
         Taq polymerase kit, DNTP - Free bases (actg ready mixed)   
      $ 600.00   Restriction enzymes  $60 x 10   
      $ 300.00   Thermocycler   
      $ 500.00   misc. equipment and supplies   
      $ 4,000.00   DNA synthesis costs $1.40 per base.   
        Protein coding regions are about 700 to 1000 bases long  This budget is for 3 - 4 new proteins.
Personal tools
Past/present/future years