Brown:Equipment needed

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| ampicillin
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| kanamycin
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| puromycin
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| Agarose
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| Ethidium Bromide
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| tris[hydroxymethyl]aminomethane
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| Acetate
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| EDTA
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| Gel Loading Dye
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| DNA ladder
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| Restriction enzymes and Buffers
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| EcoRI
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| SpeI
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| XbaI
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| PstI
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| Taq Polymerase (Master Mix)
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| 100% ethanol
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| Socium Acetate
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| NaCl
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| T4 DNA ligase
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| T4 DNA ligase Buffer
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| liquid LB Broth
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| LB with agar
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| TSS Buffer
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| Mini-Prep Kit
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| Maxi-Prep Kit
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Revision as of 06:46, 27 May 2006

type/size quantity Have(x) Location Comments
Equipment
freezers/fridges
-80°C 1
-20°C 1
4°C 1
incubator (what kind?) 1
pH meter 1
hot bath 1
cold bath 1
autoclave 1
cell shaker inside 37 degree incubator 1
source of ddH2O 1
hot plates w/ stirring 2
stir bars (set) 1
PCR thermocycler 1
microcentrifuge (w/temp cntrl.) temp. cntrl not essential 1
centrifuge 50mL and 15mL 1
Electrophoresis gel set 4
Gel Power source 1
UV Gel viewer 1
UV Gel camera 1
Pipettemen
20μL 4
200μL 4
1000μL 4
Electronic pipettor can use 1-25mL pipettes 1
Manual pipettors / bulbs 2
bunsen burner 2
sparkers 2
wire loop to pick a bac clone 3
metal loop to spread bacteria 3
Glassware
Bottles
1L 4
500mL 8
100mL 8
Beakers
1L 2
250mL 8
Centrifuge tubes
50mL 100
15mL 100
Graduated cylinders

500mL

2
100mL 2
Electronic pipette tips (packages)
1mL 2
5mL 2
10mL 2
25mL 2
pasteur pipettes 1 box
Gloves (boxes)
small 1
medium 4
large 4
Plasticware
eppendorf tubes
1.5mL 2000
200μL (thin wall for PCR) 1000
Pipette tips
20μL a lot
200μL a lot
1000μL a lot
Bacterial cell culture plates 200
bacterial cell culture tubes (polypropylene) 100
tube racks/holders
for 1.5mL tubes 4
for 200μL tubes 2
Reagents
pH standard buffers for meter 1 70% ethanol
anitibiotics
ampicillin
kanamycin
puromycin
Agarose
Ethidium Bromide
tris[hydroxymethyl]aminomethane
Acetate
EDTA
Gel Loading Dye
DNA ladder
Restriction enzymes and Buffers
EcoRI
SpeI
XbaI
PstI
Taq Polymerase (Master Mix)
100% ethanol
Socium Acetate
NaCl
T4 DNA ligase
T4 DNA ligase Buffer
liquid LB Broth
LB with agar
TSS Buffer
Mini-Prep Kit
Maxi-Prep Kit
Retrieved from "http://2006.igem.org/wiki/index.php/Brown:Equipment_needed"
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