From 2006.igem.org
| Equipment
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| freezers/fridges
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| incubator
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| hot bath
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| cold bath
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| autoclave
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| cell shaker/incubator
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| source of ddH2O
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| hot plates w/ stirring
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| stir bars (set)
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| PCR thermocycler
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| microcentrifuge (w/temp cntrl.)
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| centrifuge
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| Electrophoresis gel set
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| Gel Power source
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| UV Gel viewer
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| UV Gel camera
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| Pipettemen
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| Electronic pipettor
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| bunsen burners
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| sparkers
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| wire loop
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| metal loop
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| Glassware
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| Bottles
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| Beakers
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| Centrifuge tubes
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| Graduated cylinders
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| Electronic pipette tips (packages)
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| Gloves (boxes)
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| Plasticware
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| centrifuge tubes
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| Pipette tips
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| Bacterial cell culture plates
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| bacterial cell culture tubes
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| tube racks/holders
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$ 400.00 Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/> custom primers
$ 800.00 Antibodies for identifying cell division proteins 4 x $200
$ 250.00 disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates)
$ 600.00 Gel kit: Agarose gel aparatus, power source
TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros
Taq polymerase kit, DNTP - Free bases (actg ready mixed)
$ 600.00 Restriction enzymes $60 x 10
$ 300.00 Thermocycler
$ 500.00 misc. equipment and supplies
$ 4,000.00 DNA synthesis costs $1.40 per base.
Protein coding regions are about 700 to 1000 bases long This budget is for 3 - 4 new proteins.
