Brown:Equipment needed

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Equipment type/size quantity
freezers/fridges
-80°C 1
-20°C 1
4°C| 1|- | 1|- | 1|- | 1|- | 1|- | 1|- | 1|- | 2|- | 1|- | 1|- | 1|- 50mL and 15mL| 1|- | 4|- | 1|- | 1|- | 1|- | |- 20μL| 4|- 200μL| 4|- 1000μL| 4|- can use 1-25mL pipettes| 1|- | 2|- | 2|- to pick a bac clone| 3|- to spread bacteria| 3|- | |- | |- type/size| quantity|- | |- 1L| 4|- 500mL| 8|- 100mL| 8|- | |- 1L| 2|- 250mL| 8|- | |- 50mL| 100|- 15mL| 100|- | |- 500mL| 2|- 100mL| 2|- | |- 1mL| 2|- 5mL| 2|- 10mL| 2|- 25mL| 2|- | |- small| 1|- medium| 4|- large| 4|- | |- | |- | |- | |- 1.5mL| 2000|- 200μL (PCR)| 1000|- | |- 20μL| a lot|- 200μL| a lot|- 1000μL| a lot|- | 200|- | |- (polypropylene)| 100|- | |- for 1.5mL tubes| 4|- 2|- Reagents 70% ethanol
      $ 400.00   Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>   custom primers   
      $ 800.00   Antibodies for identifying cell division proteins 4 x $200   
      $ 250.00   disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates)   
      $ 600.00   Gel kit: Agarose gel aparatus, power source   
         TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros   
         Taq polymerase kit, DNTP - Free bases (actg ready mixed)   
      $ 600.00   Restriction enzymes  $60 x 10   
      $ 300.00   Thermocycler   
      $ 500.00   misc. equipment and supplies   
      $ 4,000.00   DNA synthesis costs $1.40 per base.   
        Protein coding regions are about 700 to 1000 bases long  This budget is for 3 - 4 new proteins.
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