Brown:Projects

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== Bacterial Freeze Tag ==
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<h3>Brown 2006 iGEM entry </h3>
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== [[Brown:2006/Magnetotactic Bacteria project]] ==
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==[[Overview]]==
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As the race to the finish draws near for iGEM 2006, our team has compiled a detailed summary of our summers work.  This includes our initial ideas, designs, models and the parts that we have added to the registry.  We began the summer with grand plans to design a game of bacterial freeze tag, modeled after the popular children's game where one player tries to catch the other. Our efforts were then switched to the extension of the bi-stable toggle switch and the design of a tri-stable toggle switch. Our projects highlight the team's creative and innovative approaches to designing and engineering working biological systems.
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This project involves programming bacteria to be able to play a game of freeze tag. Bacteria will be engineered to swim around a microfluidics device until they reach a certain proximity to the 'IT' cell and then they will lose their ability to move. This loss of motility will be combined with a change in color from Green to Blue. When another bacterium, which is moving (not the 'IT' cell), reaches a certain proximity to the 'frozen' bacteria it will again regain its ability to move and turn from Blue to Yellow.
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==[[Sender Cell]]==
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Click here to see our BioBricks! [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006&group=Brown Brown iGEM 2006 Parts]
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TetR promoted with LuxI downstream. LuxI is an enzyme that produces AHL and will produce the red fluorescent protein (RFP). The AHL produced is exported from the cell where it then forms a complex with the LuxR protein that is produced by the AHL sensor within the Receiver cell.
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[[Image:SenderCell.png|frame|AHL Sender Cell]]
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<h3>[[Brown:Bacterial Freeze Tag | Bacterial Freeze Tag]] </h3>
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==[[AHL Sensor]]==
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*[[Brown:Bacterial_Freeze_Tag#Overview |Overview]]
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*[[Brown:Bacterial_Freeze_Tag#Sender cell | Sender cell ]]
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*[[Brown:Bacterial_Freeze_Tag#Receiver cell | Receiver cell ]]
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*[[Brown:Bacterial_Freeze_Tag#Challenges| Challenges ]]
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The AHL sensor is TetR promoted and forms the LuxR protein which then forms a complex with AHL. This LuxR and AHL complex then activates the pLuxR promoter. Downstream of the pLuxR promoter is the LacI protein. LacI inhibits the pLac promoter on the "Freeze Machine".
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[[Image:AHLSensor.jpg|frame|AHL Sensor]]
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==[['Freeze Machine']]==
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<h3>[[Brown:Tri-Stable toggle switch|Tri-Stable toggle switch]]</h3>
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A promoter that is regulated by LacI will promote the production of LasI, MotB, and cI. This will subsequently inhibit the production of CFP and LasR. In the presence of LacI, however, MotB, LasI, and cI will not be produced. CFP will therefore be produced along with LasR and LacI. This results in the "freezing" of the cell.
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*[[Brown:Tri-Stable_toggle_switch|Introduction to the project]]
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[[Image:FreezeMachine.png|frame|Freeze Machine]]
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*[[Brown:Tri-Stable toggle switch#Modeling the tri-stable toggle switch | Modeling the tri-stable switch]]
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*[[Brown:Tri-Stable toggle switch#What we achieved| What we achieved]]
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*[[Brown:Tri-Stable toggle switch#Challenges | Challenges ]]
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*[[Brown:Tri-Stable toggle switch#Challenges | Future work ]]
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==[["Dr. Unfreeze"]]==
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"Dr. Unfreeze" is responsible for short circuiting the freeze machine.  When it is activated by AI-1 and LasR it begins producing cI, which will inhibit pcI and allow the cell to begin producing MotB.
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[[Image:unfreeze.png|frame|Dr. Unfreeze]]
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<h3>[[Brown:Team logistics | Team Logistics ]] </h3>
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*[[Brown:Team logistics|How we got here]]
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*[[Brown:Team_logistics|Challenges]]
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*[[Brown:Team_logistics|Future work]]
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__NOTOC__

Latest revision as of 03:04, 2 November 2006

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Brown 2006 iGEM entry

As the race to the finish draws near for iGEM 2006, our team has compiled a detailed summary of our summers work. This includes our initial ideas, designs, models and the parts that we have added to the registry. We began the summer with grand plans to design a game of bacterial freeze tag, modeled after the popular children's game where one player tries to catch the other. Our efforts were then switched to the extension of the bi-stable toggle switch and the design of a tri-stable toggle switch. Our projects highlight the team's creative and innovative approaches to designing and engineering working biological systems.

Click here to see our BioBricks! Brown iGEM 2006 Parts

Bacterial Freeze Tag

Tri-Stable toggle switch

Team Logistics



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