Brown:Projects

From 2006.igem.org

(Difference between revisions)
Jump to: navigation, search
Line 9: Line 9:
TetR promoted with LuxI downstream. LuxI is an enzyme that produces AHL and will produce the red fluorescent protein (RFP). The AHL produced is exported from the cell where it then forms a complex with the LuxR protein that is produced by the AHL sensor within the Receiver cell.
TetR promoted with LuxI downstream. LuxI is an enzyme that produces AHL and will produce the red fluorescent protein (RFP). The AHL produced is exported from the cell where it then forms a complex with the LuxR protein that is produced by the AHL sensor within the Receiver cell.
-
[[Image:SenderCell.png]]
+
[[Image:SenderCell.png|frame|AHL Sender Cell]]
==[[AHL Sensor]]==
==[[AHL Sensor]]==

Revision as of 17:43, 13 July 2006

CSAIL logo

Home        People        Research        iGEM Proposal        Coming to Brown?        Contact        Projects              

Contents

Bacterial Freeze Tag

Overview

This project involves programming bacteria to be able to play a game of freeze tag. Bacteria will be engineered to swim around a microfluidics device until they reach a certain proximity to the 'IT' cell and then they will lose their ability to move. This loss of motility will be combined with a change in color from Green to Blue. When another bacterium, which is moving (not the 'IT' cell), reaches a certain proximity to the 'frozen' bacteria it will again regain its ability to move and turn from Blue to Yellow.

Sender Cell

TetR promoted with LuxI downstream. LuxI is an enzyme that produces AHL and will produce the red fluorescent protein (RFP). The AHL produced is exported from the cell where it then forms a complex with the LuxR protein that is produced by the AHL sensor within the Receiver cell.

AHL Sender Cell

AHL Sensor

The AHL sensor is TetR promoted and forms the Lux R protien which then forms a complex with AHL. This LuxR and AHL complex then activates the pLUXR promoter. Downstream of the pLUXR promoter is the LACI protein. LacI inhibit the pLAC promoter on the "Freeze Machine".

'Freeze Machine'

A promoter that is regulated by LacI will promote the production of LasI, MotB, and cI. This will subsequently inhibit the production of CFP and LasR. In the presence of LacI, however, MotB, LasI, and cI will not be produced. CFP will therefore be produced along with LasR and LacI. This results in the "freezing" of the cell.

Unfreeze Mechanism

Magnetotactic Bacteria

Personal tools
Past/present/future years