Construction
From 2006.igem.org
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Revision as of 01:31, 9 October 2006
< Aug11-Sep7 | Sep8-14 | Sep15-26 | Sep27-Oct3 | Construction >
October 7, 2006
Cheng:
- Prepared DH5a-z1 UT2 for IPTG induction of GFP
Conrad:
- Re-test UT4C, UT5 ABC and I12006 EFG (2005) with XbaI/SpeI
- Miniprep UT2/3 in DH5a
Siva:
- Prepared DH5a UT2 for Arabinose induced LacI test
Recorded digest length check results:
- I12006 EFG (4000-5000, 1500-1000)
- UT5 ABC (3500-4000, 750-1000)
- UT4 C (3000-3500)
- UT3 DH5a (750-1000, 2000-2500)
- UT2 DH5a (750-1000, 2000-2500)
Test Results
October 6, 2006
Andy, HoKwon:
- Prepared o/n (3 vials each) of DH5a and DH5a-z1 with UT2 and UT3 for testing
- Prepared o/n of UT2 DH5a and UT3 DH5a for miniprep
- Transformed and plated I12006 (2005) (poured in amp plate first by accident), I12006 (2006)
- Double Digest parts E0240 CD (2005), UT4 AB with XbaI/SpeI
- E0240 CD (2000-2500, 750-1000) Correct!
- UT4 AB (3000-4000) Correct!
Charles, Cheng, Nick:
- Re-tested UT4 C, UT5 ABC – failed due to a very dry gel
October 4, 2006
Andy:
- Checked lengths (EcoRI, PstI) of following
- I12006 EFG (4000-5000, 1000-1500 for all three)
- UT4 AB (3000-4000, 2000) C (3000-4000, 250)
- UT5 ABC (2500-3000, 750-1000)
- Digested following
- I12006 (2005) E (6000 by SpeI and PstI)
- E0240 (2005) C (2500-3000, 2000-2500, 1500-2000, 750-1000 by XbaI and PstI)
- UT4 AB (6000-10000, 2000-2500 by SpeI and PstI) C (5000-6000, 3000 by SpeI and PstI)
- UT5 ABC (3000-4000 by SpeI and PstI)
- Gel extracted E0240 (insert) and UT4 AB (host)
- Transformed and plated DH5a with UT2/UT3 for testing
- Made o/n of UT2/UT3 with IPTG
To-Do:
- Redo transformation of I12006 (2005)
- Learn to take fluorescent images under microscope
- Temperature testing
- Try ligating UT5 again
- Running out of water and PCR tubes
