ETH2006 xor

From 2006.igem.org

Contents 1 XOR gate 1.1 implementation 1.1.1 implementation alternatives 1.1.2 current 1.2 modeling 1.2.1 ideal xor gate 1.2.2 early simulation results of variants 1.2.3 current simulation results 1.2.4 sensitivity analysis 1.2.5 more (old) simulation results 1.3 assembly procedure 1.4 test procedure 1.5 test results 1.6 parts

XOR gate

The XOR gate's PoPs output activity should be correlated to the PoPs input activity as shown in the picture:

 input A ^
         | H  D  L
         | D  D  D
         | L  D  H
         +--------->
            input B

 output: High, Low, Dont care

implementation

implementation alternatives

when we knew that we were going to implement an XOR, we came up with different artificial variants, without knowing whether such a system would exist in biology or not. while our biologists started searching the literature, we modelled the variants to see which we might want to favor.

here, we pick out 2 variants with corresponding simulation results to give an idea of the process of development. note that at this stage, the models still contain minor inaccuracies such as missing ribosome binding sites, which we corrected later on.

further development of the first variant resulted in the current implementation of the XOR gate.

current

Part to be used in the prototype system: [http://partsregistry.org/Part:BBa_J34200 BBa_J34200]

modeling

we are interested in the input/output behavior at steady state, that is when the system has reached equilibra and the concentrations of concerned species don't change anymore. since we have 2 inputs and 1 ouptut, simulation results will be illustrated as a surface plot, inputs on the x/y axes and output on the z axis.

ideal xor gate

the expected input/output behavior of an ideal xor gate looks like this (with less green being better):

note that the anterior low (blue) part has low inputs, the rear one has high inputs.

early simulation results of variants

the following figures show early simulation results of the implementation variants 1 and 2. note that also the models contain inexactness and even defects at this stage.

Variant 1	align="center" Variant 2
surface plot of simulation results for XOR variants 1 and 2

current simulation results

sensitivity analysis

more (old) simulation results

simulation results / sensitivity analysis

assembly procedure

test procedure

test results

parts

[http://partsregistry.org/Part:BBa_J34100 BBa_J34100]

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