Flip One Pancake

From 2006.igem.org

(Difference between revisions)
Jump to: navigation, search
Line 7: Line 7:
#  RE (recombination enhancer), synthesized by Davidson team
#  RE (recombination enhancer), synthesized by Davidson team
#  One Pancake test construct:
#  One Pancake test construct:
-
  <br> araBAD promoter, inducible with arabinose
+
<br> araBAD promoter, inducible with arabinose
-
      b. RBS
+
<br> RBS
-
      c. hixL recombination site, synthesized by Missouri Western team
+
<br> hixL recombination site, synthesized by Missouri Western team
-
      d. CDS for mRFP in forward direction
+
<br> CDS for mRFP in forward direction
-
      e. hixR recombination site, synthesized by Missouri Western team
+
<br> hixR recombination site, synthesized by Missouri Western team
-
      f. double forward terminator
+
<br> double forward terminator

Revision as of 12:25, 7 June 2006

Flipping One Pancake

The plan is to construct a plasmid that contains the following:

  1. Amp resistance gene as in pSB1A3
  2. Hin recombinase gene driven by lacP (inducible in JM109 by IPTG or lactose), cloned from PCR product by Davidson team
  3. RE (recombination enhancer), synthesized by Davidson team
  4. One Pancake test construct:


araBAD promoter, inducible with arabinose
RBS
hixL recombination site, synthesized by Missouri Western team
CDS for mRFP in forward direction
hixR recombination site, synthesized by Missouri Western team
double forward terminator

Personal tools
Past/present/future years