Flip One Pancake

From 2006.igem.org

Revision as of 12:24, 7 June 2006 by Eckdahl (Talk | contribs)
Jump to: navigation, search

Flipping One Pancake

The plan is to construct a plasmid that contains the following:

  1. Amp resistance gene as in pSB1A3
  2. Hin recombinase gene driven by lacP (inducible in JM109 by IPTG or lactose), cloned from PCR product by Davidson team
  3. RE (recombination enhancer), synthesized by Davidson team
  4. One Pancake test construct:
 
araBAD promoter, inducible with arabinose b. RBS c. hixL recombination site, synthesized by Missouri Western team d. CDS for mRFP in forward direction e. hixR recombination site, synthesized by Missouri Western team f. double forward terminator
Personal tools
Past/present/future years