IGEM06 DNA Distribution Issues

From 2006.igem.org

Revision as of 20:16, 5 July 2006 (view source) Ljw140 (Talk | contribs) ← Older edit		Revision as of 19:30, 20 July 2006 (view source) Macampbell (Talk | contribs) Newer edit →
Line 29:		Line 29:
	|iGEM06/05 DNA-1		|iGEM06/05 DNA-1
	|neither amp plate seems to work		|neither amp plate seems to work
		+	|}
		+
		+	{| border="1" cellpadding="5"
		+	!<u>Team</u> || <u>Part number</u> !! <u>Plasmid</u> !! <u>Cell strain</u> !! <u>Distribution plate</u> !! <u>Comments</u>
		+	|-
		+	|Davidson
		+	|BBa_B0015
		+	|pSB1A2,
		+	|JM109
		+	|iGEM06/05 DNA-1
		+	|no insert at all
	|}		|}

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Revision as of 19:30, 20 July 2006

This page is to keep a record of any troubles that you have when trying to successfully transform parts from either of the two iGEM 2006 DNA Distribution plates (See Biobrick Delivery page for delivery details)

Please include:

• Biobrick part number
• Plasmid
• Cell strain used
• Plate (iGEM 2006 DNA-1 or DNA-2) and well number

Team	Part number	Plasmid	Cell strain	Distribution plate	Comments
Example	BBa_xxxx	pSB1A2	TOP10	iGEM06 DNA-1	we tried 10 times!!

Team	Part number	Plasmid	Cell strain	Distribution plate	Comments
PennState	BBa_R0051	pSB1A2, pSB2K3	DH5a	iGEM06/05 DNA-1	neither amp plate seems to work

Team	Part number	Plasmid	Cell strain	Distribution plate	Comments
Davidson	BBa_B0015	pSB1A2,	JM109	iGEM06/05 DNA-1	no insert at all

If we see the same Biobrick parts giving multiple teams trouble it will give us a better sense of what we need to fix.

Note: Please take a look and see if your team has been able to successfully transform any of the parts on this list. You might want to contact the team that was having trouble and let them know that you were able to successfully transform that particular part. All of the distribution plates were done in the same method at the same time so they should be the same, but that isn't always the case.