Lab Work

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(11th July 2006)
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===11th July 2006===
===11th July 2006===
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Today we tested the timed pH response in 2 cell cultures:  1 with the LacZ gene, which was activated by IPTG, and one that had this gene abscent.
===10th July 2006===
===10th July 2006===

Revision as of 16:23, 11 July 2006

11th July 2006

Today we tested the timed pH response in 2 cell cultures: 1 with the LacZ gene, which was activated by IPTG, and one that had this gene abscent.

10th July 2006

The colonies transformed on the 6th made it this time, and we isolated the plasmid DNA from three individual colonies for each biobrick. We also transformed some E. coli with

23E pSB1A3 Plasmid Plate 1 AmpR

To serve as an empty plasmid for the new LacZ and arsenic promoter/repressor parts which we will create.

7th July 2006

Acid Production with LacZ and Lactose

When bacteria with the lacZ gene inserted are present in a medium containing lactose, the pH does drop significantly.








6th July 2006

Unfortunately the colonies we plated on the 4th did not survive due to a problem with the competent cells we used, so today we repeated transforming and plating colonies containing the following parts:

9E BBa_E0033 LacZ alpha Plate 2 KanR
1I BBa_B0015 Terminator Plate 1 AmpR
7K BBa_R0010 IPTG responsive promoter Plate 1 AmpR
3O BBa_B0034 RBS Plate 1 AmpR

4th July 2006

We plated colonies containing plasmids with the following parts:

9E BBa_E0033 LacZ alpha Plate 2 KanR
3P BBa_0010 Terminator Plate 2 AmpR
7K BBa_R0010 IPTG responsive promoter Plate 1 AmpR


[http://2006.igem.org/Standard_Protocols Standard Protocols]


[http://2006.igem.org/University_of_Edinburgh_2006 Main page]

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