Lab Work
From 2006.igem.org
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===11th July 2006=== | ===11th July 2006=== | ||
| - | Today we tested the timed pH response in 2 cell cultures: 1 with the LacZ gene, which was activated by IPTG, and one that had this gene | + | Today we tested the timed pH response in 2 cell cultures: 1 with the LacZ gene, which was activated by IPTG, and one that had this gene absent. |
===10th July 2006=== | ===10th July 2006=== | ||
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| 23E || pSB1A3 || Plasmid || Plate 1 || AmpR | | 23E || pSB1A3 || Plasmid || Plate 1 || AmpR | ||
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| - | + | to serve as an empty plasmid for the new LacZ and arsenic promoter/repressor parts which we will create. | |
===7th July 2006=== | ===7th July 2006=== | ||
Revision as of 15:13, 12 July 2006
11th July 2006
Today we tested the timed pH response in 2 cell cultures: 1 with the LacZ gene, which was activated by IPTG, and one that had this gene absent.
10th July 2006
The colonies transformed on the 6th made it this time, and we isolated the plasmid DNA from three individual colonies for each biobrick. We also transformed some E. coli with
| 23E | pSB1A3 | Plasmid | Plate 1 | AmpR |
to serve as an empty plasmid for the new LacZ and arsenic promoter/repressor parts which we will create.
7th July 2006
When bacteria with the lacZ gene inserted are present in a medium containing lactose, the pH does drop significantly.
6th July 2006
Unfortunately the colonies we plated on the 4th did not survive due to a problem with the competent cells we used, so today we repeated transforming and plating colonies containing the following parts:
| 9E | BBa_E0033 | LacZ alpha | Plate 2 | KanR |
| 1I | BBa_B0015 | Terminator | Plate 1 | AmpR |
| 7K | BBa_R0010 | IPTG responsive promoter | Plate 1 | AmpR |
| 3O | BBa_B0034 | RBS | Plate 1 | AmpR |
4th July 2006
We plated colonies containing plasmids with the following parts:
| 9E | BBa_E0033 | LacZ alpha | Plate 2 | KanR |
| 3P | BBa_0010 | Terminator | Plate 2 | AmpR |
| 7K | BBa_R0010 | IPTG responsive promoter | Plate 1 | AmpR |
[http://2006.igem.org/Standard_Protocols Standard Protocols]
[http://2006.igem.org/University_of_Edinburgh_2006 Main page]
