Lab Work

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7th July 2006

Acid Production with LacZ and Lactose

When bacteria with the lacZ gene inserted are present in a medium containing lactose, the pH does drop significantly.

6th July 2006

Unfortunately the colonies we plated on the 4th did not survive due to a problem with the competent cells we used, so today we repeated transforming and plating colonies containing the following parts:

9E BBa_E0033 LacZ alpha Plate 2 KanR
1I BBa_B0015 Terminator Plate 1 AmpR
7K BBa_R0010 IPTG responsive promoter Plate 1 AmpR
3O BBa_B0034 RBS Plate 1 AmpR

4th July 2006

We plated colonies containing plasmids with the following parts:

9E BBa_E0033 LacZ alpha Plate 2 KanR
3P BBa_0010 Terminator Plate 2 AmpR
7K BBa_R0010 IPTG responsive promoter Plate 1 AmpR


[http://2006.igem.org/Standard_Protocols Standard Protocols]


[http://2006.igem.org/University_of_Edinburgh_2006 Main page]

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