http://2006.igem.org/wiki/index.php?title=Ljubljana,_Slovenia_2006&feed=atom&action=historyLjubljana, Slovenia 2006 - Revision history2024-03-29T08:03:11ZRevision history for this page on the wikiMediaWiki 1.16.5http://2006.igem.org/wiki/index.php?title=Ljubljana,_Slovenia_2006&diff=21442&oldid=prevMDolinar at 16:04, 13 November 20062006-11-13T16:04:52Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| [[Image:EU-flag.gif |thumb|120px|EU Synbiocomm (thank you Sven)]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| [[Image:EU-flag.gif |thumb|120px|EU Synbiocomm (thank you Sven)]]</div></td></tr>
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</table>MDolinarhttp://2006.igem.org/wiki/index.php?title=Ljubljana,_Slovenia_2006&diff=20734&oldid=prevMDolinar at 17:10, 1 November 20062006-11-01T17:10:46Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><center><font style="font size="9" face="Arial"><b>Engineered Human Cells: <br><br><br> SAY <del class="diffchange diffchange-inline">STOP </del>TO SEPSIS</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><center><font style="font size="9" face="Arial"><b>Engineered Human Cells: <br><br><br> SAY <ins class="diffchange diffchange-inline"><font color="red">NO </font> </ins>TO SEPSIS</div></td></tr>
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</table>MDolinarhttp://2006.igem.org/wiki/index.php?title=Ljubljana,_Slovenia_2006&diff=20549&oldid=prevMDolinar at 14:46, 31 October 20062006-10-31T14:46:38Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{|</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-valign="center" align="justify"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-valign="center" align="justify"</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|border="1" solid #affaaa" cellspacing="1" cellpadding="1" style="border:1px solid black; background:#cedff2" | <font style="font size="3" font face="Times New Roman""><p><b>Mammalian systems can be a subject of cellular engineering similarly to bacterial cells. We decided to tinker with the existing cell signaling network of the response to the bacterial infection. Binding of bacterial components ([[Ljubljana, Slovenia 2006/Terms & References#Terms|PAMP]]s – Pathogen associated molecular patterns) to a family of Toll-like receptors activates the cells of the immune system but the exaggerated response may lead to systemic inflammation and sepsis which is often fatal. We designed a feedback loop, which inhibits the <del class="diffchange diffchange-inline">signalling </del>cascade at the »weak spot« - [[Ljubljana, Slovenia 2006/Terms & References#Terms|MyD88]], a consensus adaptor protein of the Toll-like receptors. A mathematical model of cell activation with engineered feedback loop was constructed, which predicts the decrease of the cellular activation after the repeated stimulation. Twenty-six new BrioBricks were constructed specially for the mammalian system. We have experimentally confirmed the function of the feedback device by detecting the inhibition of cellular activation after the repeated stimulation. Cell activation decreased without completely deleting the responsiveness to the bacterial infection, thus our engineered cell system represents a type of artificial immunotolerance.</b></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|border="1" solid #affaaa" cellspacing="1" cellpadding="1" style="border:1px solid black; background:#cedff2" | <font style="font size="3" font face="Times New Roman""><p><b>Mammalian systems can be a subject of cellular engineering similarly to bacterial cells. We decided to tinker with the existing cell signaling network of the response to the bacterial infection. Binding of bacterial components ([[Ljubljana, Slovenia 2006/Terms & References#Terms|PAMP]]s – Pathogen associated molecular patterns) to a family of Toll-like receptors activates the cells of the immune system but the exaggerated response may lead to systemic inflammation and sepsis which is often fatal. We designed a feedback loop, which inhibits the <ins class="diffchange diffchange-inline">signaling </ins>cascade at the »weak spot« - [[Ljubljana, Slovenia 2006/Terms & References#Terms|MyD88]], a consensus adaptor protein of the Toll-like receptors. A mathematical model of cell activation with engineered feedback loop was constructed, which predicts the decrease of the cellular activation after the repeated stimulation. Twenty-six new BrioBricks were constructed specially for the mammalian system. We have experimentally confirmed the function of the feedback device by detecting the inhibition of cellular activation after the repeated stimulation. Cell activation decreased without completely deleting the responsiveness to the bacterial infection, thus our engineered cell system represents a type of artificial immunotolerance.</b></p></div></td></tr>
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</table>MDolinarhttp://2006.igem.org/wiki/index.php?title=Ljubljana,_Slovenia_2006&diff=20535&oldid=prevMDolinar at 14:33, 31 October 20062006-10-31T14:33:26Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-valign="center" align="justify"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-valign="center" align="justify"</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|border="1" solid #affaaa" cellspacing="1" cellpadding="1" style="border:1px solid black; background:#cedff2" | <font style="font size="3" font face="Times New Roman""><p><b>Mammalian systems can be a subject of cellular engineering similarly to bacterial cells. We decided to tinker with the existing cell <del class="diffchange diffchange-inline">signalling </del>network of the response to the bacterial infection. Binding of bacterial components ([[Ljubljana, Slovenia 2006/Terms & References#Terms|PAMP]]s – Pathogen associated molecular patterns) to a family of Toll-like receptors activates the cells of the immune system but the exaggerated response may lead to systemic inflammation and sepsis which is often fatal. We designed a feedback loop, which inhibits the signalling cascade at the »weak spot« - [[Ljubljana, Slovenia 2006/Terms & References#Terms|MyD88]], a consensus adaptor protein of the Toll-like receptors. A mathematical model of cell activation with engineered feedback loop was constructed, which predicts the decrease of the cellular activation after the repeated stimulation. Twenty-six new BrioBricks were constructed specially for the mammalian system. We have experimentally confirmed the function of the feedback device by detecting the inhibition of cellular activation after the repeated stimulation. Cell activation decreased without completely deleting the responsiveness to the bacterial infection, thus our engineered cell system represents a type of artificial immunotolerance.</b></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|border="1" solid #affaaa" cellspacing="1" cellpadding="1" style="border:1px solid black; background:#cedff2" | <font style="font size="3" font face="Times New Roman""><p><b>Mammalian systems can be a subject of cellular engineering similarly to bacterial cells. We decided to tinker with the existing cell <ins class="diffchange diffchange-inline">signaling </ins>network of the response to the bacterial infection. Binding of bacterial components ([[Ljubljana, Slovenia 2006/Terms & References#Terms|PAMP]]s – Pathogen associated molecular patterns) to a family of Toll-like receptors activates the cells of the immune system but the exaggerated response may lead to systemic inflammation and sepsis which is often fatal. We designed a feedback loop, which inhibits the signalling cascade at the »weak spot« - [[Ljubljana, Slovenia 2006/Terms & References#Terms|MyD88]], a consensus adaptor protein of the Toll-like receptors. A mathematical model of cell activation with engineered feedback loop was constructed, which predicts the decrease of the cellular activation after the repeated stimulation. Twenty-six new BrioBricks were constructed specially for the mammalian system. We have experimentally confirmed the function of the feedback device by detecting the inhibition of cellular activation after the repeated stimulation. Cell activation decreased without completely deleting the responsiveness to the bacterial infection, thus our engineered cell system represents a type of artificial immunotolerance.</b></p></div></td></tr>
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</table>MDolinarhttp://2006.igem.org/wiki/index.php?title=Ljubljana,_Slovenia_2006&diff=20534&oldid=prevMDolinar at 14:32, 31 October 20062006-10-31T14:32:52Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><tr><th>[[Ljubljana, Slovenia 2006/Background and Signalling Pathway|Background and <del class="diffchange diffchange-inline">Signalling </del>Pathway]]</th></tr></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><tr><th>[[Ljubljana, Slovenia 2006/Background and Signalling Pathway|Background and <ins class="diffchange diffchange-inline">Signaling </ins>Pathway]]</th></tr></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><tr><th>[[Ljubljana, Slovenia 2006/Project & Model|Project & Model]]</th></tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><tr><th>[[Ljubljana, Slovenia 2006/Project & Model|Project & Model]]</th></tr></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><tr><th>[[Ljubljana, Slovenia 2006/Methods|Methods]]</th></tr></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><tr><th>[[Ljubljana, Slovenia 2006/Methods|Methods]]</th></tr></div></td></tr>
</table>MDolinarhttp://2006.igem.org/wiki/index.php?title=Ljubljana,_Slovenia_2006&diff=20517&oldid=prevRtkavc at 10:02, 31 October 20062006-10-31T10:02:10Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><center><font style="font size="9" face="Arial"><b>Engineered Human Cells: <br><br><br> SAY <del class="diffchange diffchange-inline">NO </del>TO SEPSIS</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><center><font style="font size="9" face="Arial"><b>Engineered Human Cells: <br><br><br> SAY <ins class="diffchange diffchange-inline">STOP </ins>TO SEPSIS</div></td></tr>
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</table>Rtkavchttp://2006.igem.org/wiki/index.php?title=Ljubljana,_Slovenia_2006&diff=19921&oldid=prevMDolinar at 12:40, 30 October 20062006-10-30T12:40:37Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><center><font style="font size="9" face="<del class="diffchange diffchange-inline">Times New Roman</del>"><b>Engineered Human Cells: <br><br><br> SAY NO TO SEPSIS</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><center><font style="font size="9" face="<ins class="diffchange diffchange-inline">Arial</ins>"><b>Engineered Human Cells: <br><br><br> SAY NO TO SEPSIS</div></td></tr>
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</table>MDolinarhttp://2006.igem.org/wiki/index.php?title=Ljubljana,_Slovenia_2006&diff=19887&oldid=prevRtkavc at 11:52, 30 October 20062006-10-30T11:52:48Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-valign="center" align="justify"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-valign="center" align="justify"</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|border="<del class="diffchange diffchange-inline">5</del>" solid #affaaa" cellspacing="1" cellpadding="1" style="border:1px solid black; background:#cedff2" | <font style="font size="3" font face="Times New Roman""><p><b>Mammalian systems can be a subject of cellular engineering similarly to bacterial cells. We decided to tinker with the existing cell signalling network of the response to the bacterial infection. Binding of bacterial components ([[Ljubljana, Slovenia 2006/Terms & References#Terms|PAMP]]s – Pathogen associated molecular patterns) to a family of Toll-like receptors activates the cells of the immune system but the exaggerated response may lead to systemic inflammation and sepsis which is often fatal. We designed a feedback loop, which inhibits the signalling cascade at the »weak spot« - [[Ljubljana, Slovenia 2006/Terms & References#Terms|MyD88]], a consensus adaptor protein of the Toll-like receptors. A mathematical model of cell activation with engineered feedback loop was constructed, which predicts the decrease of the cellular activation after the repeated stimulation. Twenty-six new BrioBricks were constructed specially for the mammalian system. We have experimentally confirmed the function of the feedback device by detecting the inhibition of cellular activation after the repeated stimulation. Cell activation decreased without completely deleting the responsiveness to the bacterial infection, thus our engineered cell system represents a type of artificial immunotolerance.</b></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|border="<ins class="diffchange diffchange-inline">1</ins>" solid #affaaa" cellspacing="1" cellpadding="1" style="border:1px solid black; background:#cedff2" | <font style="font size="3" font face="Times New Roman""><p><b>Mammalian systems can be a subject of cellular engineering similarly to bacterial cells. We decided to tinker with the existing cell signalling network of the response to the bacterial infection. Binding of bacterial components ([[Ljubljana, Slovenia 2006/Terms & References#Terms|PAMP]]s – Pathogen associated molecular patterns) to a family of Toll-like receptors activates the cells of the immune system but the exaggerated response may lead to systemic inflammation and sepsis which is often fatal. We designed a feedback loop, which inhibits the signalling cascade at the »weak spot« - [[Ljubljana, Slovenia 2006/Terms & References#Terms|MyD88]], a consensus adaptor protein of the Toll-like receptors. A mathematical model of cell activation with engineered feedback loop was constructed, which predicts the decrease of the cellular activation after the repeated stimulation. Twenty-six new BrioBricks were constructed specially for the mammalian system. We have experimentally confirmed the function of the feedback device by detecting the inhibition of cellular activation after the repeated stimulation. Cell activation decreased without completely deleting the responsiveness to the bacterial infection, thus our engineered cell system represents a type of artificial immunotolerance.</b></p></div></td></tr>
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</table>Rtkavchttp://2006.igem.org/wiki/index.php?title=Ljubljana,_Slovenia_2006&diff=19886&oldid=prevRtkavc at 11:52, 30 October 20062006-10-30T11:52:02Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-valign="center" align="justify"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-valign="center" align="justify"</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|border="5" solid #affaaa" cellspacing="1" cellpadding="1" style="border:1px solid black; background:#cedff2" | <font style="font size="3" font face="Times New Roman""><p><b>Mammalian systems can be a subject of cellular engineering similarly to bacterial cells. We decided to tinker with the existing cell signalling network of the response to the bacterial infection. Binding of bacterial components ([[Ljubljana, Slovenia 2006/Terms & References#Terms|PAMP]]s – Pathogen associated molecular patterns) to a family of Toll-like receptors activates the cells of the immune system but the exaggerated response may lead to systemic inflammation and sepsis which is often fatal. We designed a feedback loop, which inhibits the signalling cascade at the »weak spot« - [[Ljubljana, Slovenia 2006/Terms & References#Terms|MyD88]], a consensus adaptor protein of the Toll-like receptors. A mathematical model of cell activation with engineered feedback loop was constructed, which predicts the decrease of the cellular activation after the repeated stimulation. Twenty-six new BrioBricks were constructed specially for the mammalian system. We have experimentally confirmed the function of the feedback device by detecting the inhibition of cellular activation after the repeated stimulation. Cell activation decreased without completely deleting the responsiveness to the bacterial infection, thus our engineered cell system represents a type of artificial immunotolerance.</b></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|border="5" solid #affaaa" cellspacing="1" cellpadding="1" style="border:1px solid black; background:#cedff2" | <font style="font size="3" font face="Times New Roman""><p><b>Mammalian systems can be a subject of cellular engineering similarly to bacterial cells. We decided to tinker with the existing cell signalling network of the response to the bacterial infection. Binding of bacterial components ([[Ljubljana, Slovenia 2006/Terms & References#Terms|PAMP]]s – Pathogen associated molecular patterns) to a family of Toll-like receptors activates the cells of the immune system but the exaggerated response may lead to systemic inflammation and sepsis which is often fatal. We designed a feedback loop, which inhibits the signalling cascade at the »weak spot« - [[Ljubljana, Slovenia 2006/Terms & References#Terms|MyD88]], a consensus adaptor protein of the Toll-like receptors. A mathematical model of cell activation with engineered feedback loop was constructed, which predicts the decrease of the cellular activation after the repeated stimulation. Twenty-six new BrioBricks were constructed specially for the mammalian system. We have experimentally confirmed the function of the feedback device by detecting the inhibition of cellular activation after the repeated stimulation. Cell activation decreased without completely deleting the responsiveness to the bacterial infection, thus our engineered cell system represents a type of artificial immunotolerance.</b></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>| [[Image:sepsa.png|450px|<del class="diffchange diffchange-inline">thumb</del>|<del class="diffchange diffchange-inline">Infection can lead to sepsis, which is in many cases fatal, but the negative feedback would decrease the imune response.</del>]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>| [[Image:sepsa.png|450px|<ins class="diffchange diffchange-inline">right</ins>|]]</div></td></tr>
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</table>Rtkavchttp://2006.igem.org/wiki/index.php?title=Ljubljana,_Slovenia_2006&diff=19813&oldid=prevRtkavc at 10:24, 30 October 20062006-10-30T10:24:06Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-valign="center" align="justify"</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>|-valign="center" align="justify"</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>|border="5" solid #affaaa" cellspacing="1" cellpadding="1" style="border:1px solid black; background:#cedff2" | <font style="font size="3" font face="Times New Roman""> <b>Mammalian systems can be a subject of cellular engineering similarly to bacterial cells. We decided to tinker with the existing cell signalling network of the response to the bacterial infection. Binding of bacterial components ([[Ljubljana, Slovenia 2006/Terms & References#Terms|PAMP]]s – Pathogen associated molecular patterns) to a family of Toll-like receptors activates the cells of the immune system but the exaggerated response may lead to systemic inflammation and sepsis which is often fatal. We designed a feedback loop, which inhibits the signalling cascade at the »weak spot« - [[Ljubljana, Slovenia 2006/Terms & References#Terms|MyD88]], a consensus adaptor protein of the Toll-like receptors. A mathematical model of cell activation with engineered feedback loop was constructed, which predicts the decrease of the cellular activation after the repeated stimulation. Twenty-six new BrioBricks were constructed specially for the mammalian system. We have experimentally confirmed the function of the feedback device by detecting the inhibition of cellular activation after the repeated stimulation. Cell activation decreased without completely deleting the responsiveness to the bacterial infection, thus our engineered cell system represents a type of artificial immunotolerance.</b></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>|border="5" solid #affaaa" cellspacing="1" cellpadding="1" style="border:1px solid black; background:#cedff2" | <font style="font size="3" font face="Times New Roman""<ins class="diffchange diffchange-inline">><p</ins>><b>Mammalian systems can be a subject of cellular engineering similarly to bacterial cells. We decided to tinker with the existing cell signalling network of the response to the bacterial infection. Binding of bacterial components ([[Ljubljana, Slovenia 2006/Terms & References#Terms|PAMP]]s – Pathogen associated molecular patterns) to a family of Toll-like receptors activates the cells of the immune system but the exaggerated response may lead to systemic inflammation and sepsis which is often fatal. We designed a feedback loop, which inhibits the signalling cascade at the »weak spot« - [[Ljubljana, Slovenia 2006/Terms & References#Terms|MyD88]], a consensus adaptor protein of the Toll-like receptors. A mathematical model of cell activation with engineered feedback loop was constructed, which predicts the decrease of the cellular activation after the repeated stimulation. Twenty-six new BrioBricks were constructed specially for the mammalian system. We have experimentally confirmed the function of the feedback device by detecting the inhibition of cellular activation after the repeated stimulation. Cell activation decreased without completely deleting the responsiveness to the bacterial infection, thus our engineered cell system represents a type of artificial immunotolerance.</b<ins class="diffchange diffchange-inline">></p</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| [[Image:sepsa.png|450px|thumb|Infection can lead to sepsis, which is in many cases fatal, but the negative feedback would decrease the imune response.]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>| [[Image:sepsa.png|450px|thumb|Infection can lead to sepsis, which is in many cases fatal, but the negative feedback would decrease the imune response.]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''We would like to express our thanks to the Sponsors'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''We would like to express our thanks to the Sponsors'''</div></td></tr>
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</table>Rtkavc