LoxP sites
From 2006.igem.org
Revision as of 21:14, 30 May 2006 by Fizzle6821 (Talk | contribs)
If we use CRE/Loxp mechanism for inversion we will need to create mutant LoxP sites Lox66 and Lox71. These mutant sites are created using PCR and overlapping primers. These mutant loxP sites will allow for only one inversion and prevent excision because CRE will no longer act on it.
see Plasmids in the methods section http://nar.oxfordjournals.org/cgi/content/full/30/17/e90
I also found that if CRE is strongly expressed or transiently expressed by a strong promoter excision the preffered action. http://www3.interscience.wiley.com/cgi-bin/fulltext/70001850/PDFSTART
