McGill Repressilator Results

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To be posted Saturday, October 28, 2006.
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== Results ==
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<h3>Issues</h3>
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At first we used BL21s cells to express the products from J40001 and I15004.  However, BL21s overexpress LacI. While oscillations are still expected under these circumstances, the period and nature of the oscillations are altered.
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<h3>Sumulations</h3>
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Standard simulation:
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[[image:repressilator_normal_sim.gif]]
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Simulation with extra lac:
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[[image:repressilator_extra_lac.gif]]
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<h3>Imaging Results</h3>
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[[image:repressilator_image_overtime.gif]]
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Figure 1. Synchronization with Model System 1. The cells in red begin in synch and remain so throughout the imaging
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period. The cell circled yellow does not synchronize. The cell boxed in white begins out of synch and synchronizes over time.
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<h3>Characteristics of the Oscillation</h3>
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[[image:oscillation characteristics.gif]]
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<h3>[[McGill_University_2006|Back to McGill's Main Page]]</h3>

Revision as of 16:58, 28 October 2006

Banner.gif


Contents

Results

Issues

At first we used BL21s cells to express the products from J40001 and I15004. However, BL21s overexpress LacI. While oscillations are still expected under these circumstances, the period and nature of the oscillations are altered.

Sumulations

Standard simulation:

Repressilator normal sim.gif

Simulation with extra lac:

Repressilator extra lac.gif

Imaging Results

Repressilator image overtime.gif

Figure 1. Synchronization with Model System 1. The cells in red begin in synch and remain so throughout the imaging period. The cell circled yellow does not synchronize. The cell boxed in white begins out of synch and synchronizes over time.

Characteristics of the Oscillation

Oscillation characteristics.gif

Back to McGill's Main Page

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