McGill Repressilator Results

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Results

Issues

At first we used BL21s cells to express the products from J40001 and I15004. However, BL21s overexpress LacI. While oscillations are still expected under these circumstances, the period and nature of the oscillations are altered.

Sumulations

Standard simulation:

Repressilator normal sim.gif

Simulation with extra lac:

Repressilator extra lac.gif

Imaging Results

Repressilator image overtime.gif

Figure 1. Synchronization with Model System 1. The cells in red begin in synch and remain so throughout the imaging period. The cell circled yellow does not synchronize. The cell boxed in white begins out of synch and synchronizes over time.

Characteristics of the Oscillation

Oscillation characteristics.gif

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