McGill University 2006
From 2006.igem.org
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<h3><ul><li>'''Team 1: Split YFP'''</ul></h3> | <h3><ul><li>'''Team 1: Split YFP'''</ul></h3> | ||
This project involves the joining of two proteins Jun and Fos each fused to a half terminus of YFP. | This project involves the joining of two proteins Jun and Fos each fused to a half terminus of YFP. | ||
- | Subsequently, both of these chimeric proteins were fused to a beta gene that codes for a membrane protein. | + | Subsequently, both of these chimeric proteins were fused to a beta gene that codes for a membrane protein. Two cell populations - one expressing Jun-beta-YFPN and the other, Fos-beta-YFPC - were combined and the two proteins were expressed, ideally resulting in the fusion of the Jun and Fos proteins on the cell membrane. This would result in the binding of the two halves of the YFP protein resulting in fluorescence. |
- | + | ||
- | Two cell populations - one expressing Jun-beta-YFPN and the other, Fos-beta-YFPC - were combined and the two proteins were expressed, ideally resulting in the fusion of the Jun and Fos proteins on the cell membrane. This would result in the binding of the two halves of the YFP protein resulting in fluorescence. | + | |
[[Background|Background]] | [[Background|Background]] |