McGill University 2006

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Contents

Name: Fousion

News

  • 2006-04-14 Discussion page created
  • 2006-03-16 First kick-off meeting and team selection

Organisation

People

Students

Catie Lichten (Center for Nonlinear Dynamics) Octavio Mondragon (Physics) Belinda Kong (Microbiology and Immunology) Adrian Kaats (Biomedical Engineering) Jamie Schafer (Microbiology and Immunology) Horia Vulpe (Physiology) Ashwini Bapat (Biochemistry) Aaron Lapierre (Anatomy & Cell Biology) Josh Wright (Chemistry) Jieun Kim(Biology)

Supervisors

Jay Nadeau

Timeline

Tasks

Parts shopping list

Composite bricks - these are the best to use as less cloning is required.

All these bricks express YFP and downregulate it in response to a signal

YELLOW

ARABINOSE RESPONSE.

BBa_E0610: Arabinose turns on lac repressor which in turn shuts down Yellow fluorescent protein.

BBa_E0600: like BBa_E0610 but YFP is destabilized - slow degradation. Not listed as available.

BBa_E0611: Arabinose turns on destabilized TetR repressor which shuts down yellow fluorescent protein. Addition of Tetracyline blocks TetR repressor allowing yellow fluorescent protein to be turned back on.

BBa_E0601: Like BBa_E0611 but YFP is destabilized - slow degradation. Not listed as available

BBa_E0613: Arabinose turns on destabilized lambda repressor which shuts down yellow fluorescent protein.

BBa_E0602: Like BBa_E0613 but YFP is destabilized - slow degradation. Not listed as available

BBa_E0603: Like BBa_E0602 but baseline expression of YFP is higher. Not listed as available

BBa_E0604: Arabinose turns on destabilized 434 cl epressor which in turn shuts down destabilized (slow) Yellow fluorescent protein. Not listed as available

BBa_E0614: Like BBa_E0604 but YFP is not destabilized (hangs around forever). Not listed as available

BBa_E0605: Arabinose turns on destabilized p22 epressor which in turn shuts down destabilized (slow) Yellow fluorescent protein. Not listed as available

BBa_E0615: Like BBa_E0605, but YFP is not destabilized, hangs around forever.


CYAN

TET response

BBa_I0401: Expresses CFP and lux from the Tet-OFF promoter. Neither is destabilized. Not listed as available

BBa_I0402: Like BBa_I0401 but lux is destabilized (LVA, slow)

BBa_I0404: Produces rhlR and CFP, downregulated by Tet. rhlR binds with N-butyryl-HSL, regulates transcription. rhlR but not CFP is destabilized.

Arabinose response:

BBa_I0403 Expresses CFP and aiiA from an arabinose promoter. aiiA degrades N-acyl homoserine lactones (quorum sensing autoinducers). aiiA but not CFP is destabilized (LVA, slow).

=Introduction= Our brainstorming meeting on 3-16 gave us several preliminary ideas which we will explore via modeling and research

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