McGill University 2006

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Name: Fousion

News

  • 2006-04-14 Discussion page created
  • 2006-03-16 First kick-off meeting and team selection

If there is a specific task that we need the bacteria to do (e.g. change colour on cue, aggregate...) list it here. We have a TON of composite bricks, and can plan custom ones. BUT I need to know what U want... I am going nuts looking at the list

Your Dyslexic Dinosaur

Organisation

People

Students

Catie Lichten (Center for Nonlinear Dynamics) Octavio Mondragon (Physics) Belinda Kong (Microbiology and Immunology) Adrian Kaats (Biomedical Engineering) Jamie Schafer (Microbiology and Immunology) Horia Vulpe (Physiology) Ashwini Bapat (Biochemistry) Aaron Lapierre (Anatomy & Cell Biology) Josh Wright (Chemistry) Jieun Kim(Biology)

Supervisors

Jay Nadeau

Timeline

Tasks

Parts shopping list

Composite bricks - these are the best to use as less cloning is required.

All these bricks express YFP and downregulate it in response to a signal

YELLOW

ARABINOSE RESPONSE.

BBa_E0610: Arabinose turns on lac repressor which in turn shuts down Yellow fluorescent protein.

BBa_E0600: like BBa_E0610 but YFP is destabilized - slow degradation. Not listed as available.

BBa_E0611: Arabinose turns on destabilized TetR repressor which shuts down yellow fluorescent protein. Addition of Tetracyline blocks TetR repressor allowing yellow fluorescent protein to be turned back on.

BBa_E0601: Like BBa_E0611 but YFP is destabilized - slow degradation. Not listed as available

BBa_E0613: Arabinose turns on destabilized lambda repressor which shuts down yellow fluorescent protein.

BBa_E0602: Like BBa_E0613 but YFP is destabilized - slow degradation. Not listed as available

BBa_E0603: Like BBa_E0602 but baseline expression of YFP is higher. Not listed as available

BBa_E0604: Arabinose turns on destabilized 434 cl epressor which in turn shuts down destabilized (slow) Yellow fluorescent protein. Not listed as available

BBa_E0614: Like BBa_E0604 but YFP is not destabilized (hangs around forever). Not listed as available

BBa_E0605: Arabinose turns on destabilized p22 repressor which in turn shuts down destabilized (slow) Yellow fluorescent protein. Not listed as available

BBa_E0615: Like BBa_E0605, but YFP is not destabilized, hangs around forever.


Lac I REGULATED

BBa_I12026: Produces YFP (not destabilized). Controlled by Lac I

MULTIPLE REGULATORS

BBa_I12051: Lactose induces lambda repressor. Arabinose induces 434 repressor. Lambda repressor activates YFP, 434 represses YFP. YFP is destabilized (AAV, fast).

BBa_I12052: Like BBa_I12051 but promoter for YFP has double binding sites for better control.


CYAN

TET response

BBa_I0401: Expresses CFP and lux from the Tet-OFF promoter. Neither is destabilized. Not listed as available

BBa_I0402: Like BBa_I0401 but lux is destabilized (LVA, slow)

BBa_I0404: Produces rhlR and CFP, downregulated by Tet. rhlR binds with N-butyryl-HSL, regulates transcription. rhlR but not CFP is destabilized.

BBa_I0405: same as BBa_I0404, but produces rhIl which produces N-butyryl-HSL.

BBa_I0406: produces CFP and lasR, which accepts chemical signal AI-1. Downregulated by Tet. LasR but not CFP are destabilized.

BBa_I0407. Like BBa_I0406 but produces lasI (destabilized LVA slow) which generates the chemical signal Al-1.

BBa_I0408: produces CFP and CinR, repressed by Tetracycline. Cin R is LVA (slow) destabilized. Cin R binds O3-C14:1-HSL and activates the Cin promoter.

BBa_I0409: Like BBa_I0408 but produces cinL (destabilized LVA slow) which generates the chemical signal O3-C14:1-HSL.



ARABINOSE response:

BBa_I0403 Expresses CFP and aiiA from an arabinose promoter. aiiA degrades N-acyl homoserine lactones (quorum sensing autoinducers). aiiA but not CFP is destabilized (LVA, slow).


SIGNALING

BBa_I0460 aiiA cassette. Produces enzyme which degrades N-acyl homoserine lactones (quorum sensing autoinducers. Destabilized with LVA (slow) B.Y.O.P (Bring your own promoter).

BBa_I10100: Produces adhesin, makes bacteria stick to each other. B.Y.O.P (Bring your own promoter)

BBa_I12002: Lambda promoter driving expression of 434 repressor. Not destabilized.

BBa_I12011: Lambda promoter driving expression of p22 repressor. LVA (slow) destabilized.

=Introduction= Our brainstorming meeting on 3-16 gave us several preliminary ideas which we will explore via modeling and research

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