Minutes

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'''Holidays'''
'''Holidays'''
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* Jude **8<sup>th</sup> – 16<sup>th</sup> August
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* Jude  
 +
**8<sup>th</sup> – 16<sup>th</sup> August
* Laszlo      8<sup>th</sup> – 22<sup>nd</sup> August
* Laszlo      8<sup>th</sup> – 22<sup>nd</sup> August
* Sree       24<sup>th</sup> – 25<sup>th</sup> August
* Sree       24<sup>th</sup> – 25<sup>th</sup> August

Revision as of 14:39, 8 August 2006

Contents

Week 7: 31st July - 4th August

Meeting - 2nd August

Wiki

  • Update Wiki re. primers for biobricks


Tamara’s visit

  • Team meeting at 10am in Hons room on Tuesday 8th August
  • Construct an agenda for meeting
  • Show her round the labs
  • Attend a Fringe Festival show in the evening


Cambridge visit

  • Best dates are 22nd to 24th August
  • Failing this, the start of September is okay
  • Jude currently has 3 spare rooms (possibly 4) from 22nd to 24th August
  • Invite ICL as well


Holidays

  • Jude
    • 8th – 16th August
  • Laszlo 8th – 22nd August
  • Sree 24th – 25th August
  • Jelena 27th August – 9th September
  • Kim 8th – 28th September
  • Farid  ??? September
  • Bryony  ??? September


pH Turtle

  • Currently not working. Chris suggests deleting mouse software from laptop. Will try this.


Modelling

  • Meeting on Friday 4th August with Hong Wu


Practical construction of working device

  • Kim has come up with lots of characterization experiments to be done in the lab next week once we have the arsenic-sensing device
  • Device might be ready on Friday 4th August
  • Email Andrew Meharg (author of Venomous Earth) to ask his advice on arsenic biosensing device


Website

  • Do we need a disclaimer?
  • Need to design Macteria mascot – possibly ask the guy who did the cartoon for Drew and Endy (Nature website)!


Biobricks

  • These can be added to the registry when they are ready (well done Jude who has done this before the minutes have been typed!)


Week 5: 17th - 21st July

Meeting - 18th July

Labwork on Arsenic Biosensor:

Biobricks

  • PCR on arsenic and LacZ parts
  • 4 bands on gel from LacZ so need to find out which one is the correct one
  • Create sticky ends
  • Working towards arsenic device – may be ready in about 2 weeks

pH Experiments

  • Want to measure population density at the point at which pH of blue and white E-Coli diverges
  • Difference between stationary and growth phase response is possibly due to the fact that the bacteria ‘want’ to use up the lactose when they’re in the growth phase
  • Ideal conditions for growth in LB have been established
  • Want to carry out experiments using different growth media


Model

  • Plenty of info on Lac operon
  • Paper found regarding ArsR relation to arsenic concentration
  • Autoregulation – ArsR represses itself
  • Background noise may be caused due to degradation of ArsR repressor
  • Might be possible to reduce this background noise by separating the promoter from the ArsR gene
  • Need to calculate what pH from urea breakdown will be for different concentrations
  • It is thought to be necessary to have the two-part promoter (with activator and repressor binding sites) in order to prevent a build up of alkalinity which could harm the bacteria


Wiki

  • Update biobricks


Cambridge trip

  • Train leaves Edinburgh Waverley at 9.30am so try to be there by about 9am
  • Need to prepare a presentation (half an hour long)
  • Update animation of arsenic biosensor
  • Focus is arsenic project
  • Mention 3D structure builder briefly
  • Presentation layout
    • Background – Jelena
    • Building biobricks – Jude
    • pH experiment/animation – Kim
    • Modelling – Patrick


Idea 6/11 – 3D structure builder

  • Primer design for urease
  • LacI biobrick – is it ok?


To do

  • Moving all experiments to Andrew Miller’s lab
  • Meeting on Thursday at 2pm in Honours Room
  • Website development
  • Design a badge if you have a spare five minutes
  • Presentation completed
Personal tools
Past/present/future years