Minutes

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Week 5: 17th - 21st July

Meeting - 18th July

Labwork on Arsenic Biosensor:

Biobricks

  • PCR on arsenic and LacZ parts
  • 4 bands on gel from LacZ so need to find out which one is the correct one
  • Create sticky ends
  • Working towards arsenic device – may be ready in about 2 weeks

pH Experiments

  • Want to measure population density at the point at which pH of blue and white E-Coli diverges
  • Difference between stationary and growth phase response is possibly due to the fact that the bacteria ‘want’ to use up the lactose when they’re in the growth phase
  • Ideal conditions for growth in LB have been established
  • Want to carry out experiments using different growth media


Model

  • Plenty of info on Lac operon
  • Paper found regarding ArsR relation to arsenic concentration
  • Autoregulation – ArsR represses itself
  • Background noise may be caused due to degradation of ArsR repressor
  • Might be possible to reduce this background noise by separating the promoter from the ArsR gene
  • Need to calculate what pH from urea breakdown will be for different concentrations
  • It is thought to be necessary to have the two-part promoter (with activator and repressor binding sites) in order to prevent a build up of alkalinity which could harm the bacteria


Wiki

  • Update biobricks


Cambridge trip

  • Train leaves Edinburgh Waverley at 9.30am so try to be there by about 9am
  • Need to prepare a presentation (half an hour long)
  • Update animation of arsenic biosensor
  • Focus is arsenic project
  • Mention 3D structure builder briefly
  • Presentation layout
  • Background – Jelena
  • Building biobricks – Jude
  • pH experiment/animation – Kim
  • Modelling – Patrick


Idea 6/11 – 3D structure builder

  • Primer design for urease
  • LacI biobrick – is it ok?


To do

  • Moving all experiments to Andrew Miller’s lab
  • Meeting on Thursday at 2pm in Honours Room
  • Website development
  • Design a badge if you have a spare five minutes 
  • Presentation completed

Personal tools
Past/present/future years