Minutes

From 2006.igem.org

Week 5: 17^th - 21^st July

Meeting - 18^th July

Labwork on Arsenic Biosensor:

Biobricks

• PCR on arsenic and LacZ parts
• 4 bands on gel from LacZ so need to find out which one is the correct one
• Create sticky ends
• Working towards arsenic device – may be ready in about 2 weeks

pH Experiments

• Want to measure population density at the point at which pH of blue and white E-Coli diverges
• Difference between stationary and growth phase response is possibly due to the fact that the bacteria ‘want’ to use up the lactose when they’re in the growth phase
• Ideal conditions for growth in LB have been established
• Want to carry out experiments using different growth media

Model

• Plenty of info on Lac operon
• Paper found regarding ArsR relation to arsenic concentration
• Autoregulation – ArsR represses itself
• Background noise may be caused due to degradation of ArsR repressor
• Might be possible to reduce this background noise by separating the promoter from the ArsR gene
• Need to calculate what pH from urea breakdown will be for different concentrations
• It is thought to be necessary to have the two-part promoter (with activator and repressor binding sites) in order to prevent a build up of alkalinity which could harm the bacteria

Wiki

• Update biobricks

Cambridge trip

• Train leaves Edinburgh Waverley at 9.30am so try to be there by about 9am
• Need to prepare a presentation (half an hour long)
• Update animation of arsenic biosensor
• Focus is arsenic project
• Mention 3D structure builder briefly

• Presentation layout
  • Background – Jelena
  • Building biobricks – Jude
  • pH experiment/animation – Kim
  • Modelling – Patrick

Idea 6/11 – 3D structure builder

• Primer design for urease
• LacI biobrick – is it ok?

To do

• Moving all experiments to Andrew Miller’s lab
• Meeting on Thursday at 2pm in Honours Room
• Website development
• Design a badge if you have a spare five minutes 
• Presentation completed