Missouri Western State University 2006

From 2006.igem.org

Revision as of 20:59, 1 June 2006

   

Front Row (left to right) Adam Brown, Trevor Butner, Brad Ogden, Marian Broderick, Todd Eckdahl.

Back Row (left to right) Jeff Poet, Eric Jessen, Kelly Malloy

Students

• Marian Broderick [1]

• Adam Brown [2]

• Trevor Butner [3]

• Lane Heard [4]

• Eric Jessen [5]

• Kelly Malloy [6]

• Brad Ogden [7]

• Kayla Ragsdale [8]

Faculty

• Todd Eckdahl [9], Department of Biology, [10]

• Jeff Poet [11], Department of Computer Science, Mathematics, and Physics, [12]

Shipping Address: Todd Eckdahl, Biology Department, Missouri Western State University, 4525 Downs Drive, Saint Joseph, MO, 64507

Research Links

Goodman et al. 1973-1979. The Pancake Problems [13]

Nanassy and Hughes. 1998. In Vivo Identification of Intermediate Stages of the DNA Inversion Reaction Catlyzed by the Salmonella Hin Recombinase [14]

Lim et al. 1997. Hin-mediated Inversion on Positively Supercoiled DNA [15]

Hughes et al. 1992. Sequence-specific interaction of the Salmonella Hin recombinase in both major and minor grooves of DNA [16]

Nanassy and Hughes. 2001. Hin Recombinase Mutants Functionally Disrupted in Interactions with Fis [17]

Lee et al. 1998. In Vivo Assay of Protein-Protein Interactions in Hin-Mediated DNA Inversion [18]

Merickel et al. 1998. Communication between Hin recombinase and Fis regulatory subunits during coordinate activation of Hin-catalyzed site-specific DNA inversion [19]

Haykinson and Johnson. 1993. DNA looping and the helical repeat in vitro and in vivo: effect of HU protein and enhancer location on Hin invertasome assembly [20]

Vasquez et al. 2004. Tangle Analysis of Gin Site-specific Recombination [21]

Merickel and Johnson. 2004. Topological analysis of Hin-catalysed DNA recombination in vivo and in vitro [22]

R.C. Johnson and M.F. Bruist. 1989. Intermediates in Hin-mediated DNA inversion: a role for Fis and the recombinational enhancer in the strand exchange reaction [23]

Lim and Simon. 1992. The Role of Negative Supercoiling in Hin-mediated Site-Specific Recombination[24]

The Pancake Tossing World Record[25]

Cool site for Breakfast [26]

iGEM iDeas

Powerpoint with two possible project ideas. [27]

Parts to Make:

HixL: TTCTGGAAAA CCAAGGTTTT TGATAA

HixR: TTATCAAAAA CCTTCCAAAA GGAAAA

HixC: TTATCAAAAA CCATGGTTTT TGATAA

HixC1: TTCTGGAAAACC AT CCAAAAGGAAAA

Steps

1. Design/Order DNA – Hix L, R, & C

       - Want to do a Left to Right BioB addition:  Promoter + RBS w/AmpR -->Hix site-->
         Term-->Hix(C)site-->TetR + Term

2. On receiving – restrict/ligate BioBricks into plasmid w/resistance gene on one side

    a.	Promoter, RBS BioB

    b.	TetR BioB (Backwards)

    c.	Term BioB 

3. Transform E. coli

E = G/AATC

X = T/CTAGA

P = CGAT/CG

S = A/CTAGT

hix BioBricks

Hey, it's your Davidson pals.We noticed that your biobricks do not have the Not1 site between E and X in the prefix and S and P in the suffix Thanks.

E - N - X - hix - S - N - P

HixL

GGGG GAATTC GCGGCCGC TCTAGA TTCTTGAAAACCAAGGTTTTTGATAA ACTAGT GCGGCCGC CTGCAG GGGG

HixR

GGGG GAATTC GCGGCCGC TCTAGA TTATCAAAAACCTTCCAAAAGGAAAA ACTAGT GCGGCCGC CTGCAG GGGG

HixC

GGGG GAATTC GCGGCCGC TCTAGA TTATCAAAAACCATGGTTTTTGATAA ACTAGT GCGGCCGC CTGCAG GGGG

Oligos to order

HixL_top

GGGGGAATTCGCGGCCGCTCTAGATTCTTGAAAACCAAGGTTTTTGATAAACTAGTGCGGCCGCCTGCAGGGGG

HixL_bottom

CCCCCTGCAGGCGGCCGCACTAGTTTATCAAAAACCTTGGTTTTCAAGAATCTAGAGCGGCCGCGAATTCCCCC

HixR_top

GGGGGAATTCGCGGCCGCTCTAGATTATCAAAAACCTTCCAAAAGGAAAAACTAGTGCGGCCGCCTGCAGGGGG

HixR_bottom

CCCCCTGCAGGCGGCCGCACTAGTTTTTCCTTTTGGAAGGTTTTTGATAATCTAGAGCGGCCGCGAATTCCCCC

HixC_top

GGGGGAATTCGCGGCCGCTCTAGATTATCAAAAACCATGGTTTTTGATAAACTAGTGCGGCCGCCTGCAGGGGG

HixC_bottom

CCCCCTGCAGGCGGCCGCACTAGTTTATCAAAAACCATGGTTTTTGATAATCTAGAGCGGCCGCGAATTCCCCC

Process of Creating Synthetic Pancake: