Notes on Tom Knight's talk

From 2006.igem.org

(it would be nice to podcast our talks)

1. Books that Tom Knight recommends for folks getting started
   1. Mark Ptashne's -- A Genetic Switch (get's down and dirty about the mechanisms, but also begins to talk about abstraction). Get the third edition.
   2. Benno Muller-Hill's book -- The Lac Operon.
   3. Fred Neidhardt's book on bacterial physiology. Physiology of the Bacterial Cell.
   4. Kathy Barker's book calldc "'At The Bench"' from Cold Spring Harbor Bench is a great book for naive folks walking into the lab.
   5. Methods for General and Molecular Bacteriology -- great book on culturing bacteria and keeping them alive. Aimed more at the industrial applications, but also a lot about methodology.
2. Tom's history/experience as he set out to start engineering biology.
   1. General Introduction --- initial tremendous frustation in biology. every experiment turned into two experiments. first, there was the experiment that you wanted to do. second, there was the experiment that you had to do in order to do the experiment that you wanted to do? e.g., will this restricition enzyme work with this DNA, or, is it not methylated, or something else, or something else. Basically, there are too many things to worry about. think about LEGOS. everything is designed to go together. even the flowers snap together.
   2. Standard Assembly -- Here's the link to the original article...
      Here's the link to the Registry webpage about standard assembly...
      Says that this is one arbitrary thing to do, that there are many other possibilities. The relevance of this is that somebody (i.e., Tom) has already thought through the issues that you need to deal with in order to get your cloning to always work.
      1. BioBricks have standard cloning sites that bracket each part. The sites are layed out as EcoRI, XbaI <PART> SpeI, PstI.
      2. The feature of these restriction sites is that you can take two parts A and B and make an AB or BA chimera and, having done that, you are left with a new part that has the same four bracketing restriction sites. This means that you can keep adding parts to parts and so on. This is called idempotent assembly.