Plasmid Prep

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Using overnight culture of plasmid I13522 (pTet-GFP), do plasmid prep on all 8 of the 5 ml cultures.
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Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge.  They are from overnight culture of plasmid I13522 (pTet-GFP).
Combine the eluted plasmid from all the preps into one tube and measure the yield as follows:
Combine the eluted plasmid from all the preps into one tube and measure the yield as follows:
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# measure A260 of 4 ul plasmid + 76 ul H2O
# measure A260 of 4 ul plasmid + 76 ul H2O
# this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul
# this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul
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Plasmid prep was done by Brad and Eric (Followed plasmid prep protocol), and I (Kelly) jumped in to help with the A260 readings.  We obtained 71 ng/uL.

Latest revision as of 18:55, 19 June 2006

Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. They are from overnight culture of plasmid I13522 (pTet-GFP).

Combine the eluted plasmid from all the preps into one tube and measure the yield as follows:

  1. turn on UV spec and blank with H2O
  2. measure A260 of 4 ul plasmid + 76 ul H2O
  3. this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul

Plasmid prep was done by Brad and Eric (Followed plasmid prep protocol), and I (Kelly) jumped in to help with the A260 readings. We obtained 71 ng/uL.

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