Plasmid Prep
From 2006.igem.org
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Using overnight culture of plasmid I13522 (pTet-GFP), do plasmid prep on all 8 of the 5 ml cultures. | Using overnight culture of plasmid I13522 (pTet-GFP), do plasmid prep on all 8 of the 5 ml cultures. | ||
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| + | Combine the eluted plasmid from all the preps into one tube and measure the yield as follows: | ||
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| + | # turn on UV spec and blank with H2O | ||
| + | # measure A260 of 4 ul plasmid + 76 ul H2O | ||
| + | # this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul | ||
Revision as of 14:53, 18 June 2006
Using overnight culture of plasmid I13522 (pTet-GFP), do plasmid prep on all 8 of the 5 ml cultures.
Combine the eluted plasmid from all the preps into one tube and measure the yield as follows:
- turn on UV spec and blank with H2O
- measure A260 of 4 ul plasmid + 76 ul H2O
- this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul
