Plasmid Prep
From 2006.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | + | Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. They are from overnight culture of plasmid I13522 (pTet-GFP). | |
Combine the eluted plasmid from all the preps into one tube and measure the yield as follows: | Combine the eluted plasmid from all the preps into one tube and measure the yield as follows: | ||
Revision as of 15:16, 18 June 2006
Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. They are from overnight culture of plasmid I13522 (pTet-GFP).
Combine the eluted plasmid from all the preps into one tube and measure the yield as follows:
- turn on UV spec and blank with H2O
- measure A260 of 4 ul plasmid + 76 ul H2O
- this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul
