Plasmid Prep

From 2006.igem.org

Revision as of 15:16, 18 June 2006 by Eckdahl (Talk | contribs)
Jump to: navigation, search

Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. They are from overnight culture of plasmid I13522 (pTet-GFP).

Combine the eluted plasmid from all the preps into one tube and measure the yield as follows:

  1. turn on UV spec and blank with H2O
  2. measure A260 of 4 ul plasmid + 76 ul H2O
  3. this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul
Personal tools
Past/present/future years