Plasmids and Bacteria

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*'''pSB1A3'''
 
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***amp resistant
 
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*** copy number:100-300 
 
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***size:2157 empty
 
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*'''pSB1AC3'''
 
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*** part-BBAp1000
 
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***copy number:100-3000
 
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***amp and CmR resistant
 
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***contains device that provides CmR
 
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***size:3844 ( with device) 3005 (without device)
 
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*'''pSB1AK3'''
 
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***part BBa_|13535
 
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***copy number:100-300
 
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***amp and kan resistance
 
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***contains a device for screening test
 
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***size: 3457 (with device) 3189 (without device)
 
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* Transform bacteria  '''We are using ''Salmonella typhimurium'' for the Hin protein as it was the referenced species in all of the literature.'''
* Transform bacteria  '''We are using ''Salmonella typhimurium'' for the Hin protein as it was the referenced species in all of the literature.'''
* Create RE biobrick (completed)
* Create RE biobrick (completed)
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*Isolating genomic DNA from Salmonella typhimurium (completed)
*Isolating genomic DNA from Salmonella typhimurium (completed)
*Ordered oligos for recombinational enhancer and primers for the hin gene
*Ordered oligos for recombinational enhancer and primers for the hin gene
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**These sequences will be transformed into RFP and possibly GFP plasmids
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These sequences will be transformed into RFP and possibly GFP plasmids
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*Performed Minipreps to isolate RFP and GFP plasmids to be used for transformations.

Latest revision as of 17:17, 8 June 2006

  • Transform bacteria We are using Salmonella typhimurium for the Hin protein as it was the referenced species in all of the literature.
  • Create RE biobrick (completed)
  • Isolating genomic DNA from Salmonella typhimurium (completed)
  • Ordered oligos for recombinational enhancer and primers for the hin gene
    • These sequences will be transformed into RFP and possibly GFP plasmids
  • Performed Minipreps to isolate RFP and GFP plasmids to be used for transformations.
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