Plasmids and Bacteria

From 2006.igem.org

(Difference between revisions)
Jump to: navigation, search
 
Line 1: Line 1:
-
*'''pSB1A3'''
 
-
***amp resistant
 
-
*** copy number:100-300 
 
-
***size:2157 empty
 
-
 
-
*'''pSB1AC3'''
 
-
*** part-BBAp1000
 
-
***copy number:100-3000
 
-
***amp and CmR resistant
 
-
***contains device that provides CmR
 
-
***size:3844 ( with device) 3005 (without device)
 
-
 
-
*'''pSB1AK3'''
 
-
***part BBa_|13535
 
-
***copy number:100-300
 
-
***amp and kan resistance
 
-
***contains a device for screening test
 
-
***size: 3457 (with device) 3189 (without device)
 
-
 
* Transform bacteria  '''We are using ''Salmonella typhimurium'' for the Hin protein as it was the referenced species in all of the literature.'''
* Transform bacteria  '''We are using ''Salmonella typhimurium'' for the Hin protein as it was the referenced species in all of the literature.'''
* Create RE biobrick (completed)
* Create RE biobrick (completed)
-
 
*Isolating genomic DNA from Salmonella typhimurium (completed)
*Isolating genomic DNA from Salmonella typhimurium (completed)
*Ordered oligos for recombinational enhancer and primers for the hin gene
*Ordered oligos for recombinational enhancer and primers for the hin gene
-
 
+
**These sequences will be transformed into RFP and possibly GFP plasmids
-
These sequences will be transformed into RFP and possibly GFP plasmids
+
*Performed Minipreps to isolate RFP and GFP plasmids to be used for transformations.
-
*Doing Minipreps to isolate the RFP and GFP plasmids
+

Latest revision as of 17:17, 8 June 2006

  • Transform bacteria We are using Salmonella typhimurium for the Hin protein as it was the referenced species in all of the literature.
  • Create RE biobrick (completed)
  • Isolating genomic DNA from Salmonella typhimurium (completed)
  • Ordered oligos for recombinational enhancer and primers for the hin gene
    • These sequences will be transformed into RFP and possibly GFP plasmids
  • Performed Minipreps to isolate RFP and GFP plasmids to be used for transformations.
Personal tools
Past/present/future years