Sorting Bacteria

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Contents

Intro

Emergent sorting.

Principle

Two populations of bacteria with different clustering behavior and colors.

The Aggregation approach

Basic Idea

The idea would be to have two types of E. coli : Type G would express GFP (they look green), other type R would express RFP (they look red)

The starting material is an homogeneous mixture of red and green, i.e. of G- and R-type bacteria.

The G-type bacteria would start, under induction, to express an aggregation factor, e.g. Antigen 43 (Ag43) working on stable media culture. Thus, the G-type bacteria would start to gather forming green dots on the plate.

The R-type bacteria express their own aggregation factor (Ag43), but with a certain delay and thus leaving time to the G-type bacteria to sufficiently cluster first.

This would produce a sorting behavior, i.e. green bacteria surrounded by red bacteria the latter probably forming rings.

possible Extensions

Discussion

>> for comments, questions and temporary remarks go to the Talk:Sorting_Bacteria

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