http://2006.igem.org/wiki/index.php?title=Special:Contributions&feed=atom&limit=250&target=Agao&year=&month=2006.igem.org - User contributions [en]2024-03-28T19:58:20ZFrom 2006.igem.orgMediaWiki 1.16.5http://2006.igem.org/wiki/index.php/Brown:Journal_club:Synthetic_biology_journal_clubBrown:Journal club:Synthetic biology journal club2006-11-28T05:04:55Z<p>Agao: /* November 29, 2006 */</p>
<hr />
<div>{{Brown navigation bar}} <br><br />
==25 July==<br />
Annie to present article titled "Synthetic protein-protein interatction domains created by shuffling Cys2His2 zinc-fingers".<br><br />
[http://www.nature.com/msb/journal/v2/n1/full/msb4100053.html Link to Article]<br />
<br />
==18 July==<br />
<br />
Jason to present on Article titled ''Environmentally Controlled Invasion of Cancer<br />
Cells by Engineered Bacteria''. <br><br />
<br />
[http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WK7-4HJRXGD-4-3&_cdi=6899&_user=489286&_orig=search&_coverDate=01%2F27%2F2006&_qd=1&_sk=996449995&view=c&wchp=dGLbVzb-zSkWA&md5=d973cfd361022b29e92d1ccc48c28b92&ie=/sdarticle.pdf PDF of Article]<br />
<br />
=='''Archive'''==<br />
==11 July==<br />
Brendan to present on Article titled ''Ultrasensitivity and Nosie Propagation in a Synthetic Transcriptional Cascade''. <br><br />
<br />
[http://www.pnas.org/cgi/reprint/102/10/3581 PDF of Article]<br />
<br />
==5 July==<br />
Peter presents an article about transcriptional regulators used to form an oscillator. The article is [http://sas.epnet.com/externalframe.asp?tb=0&_ug=sid+22B5129D%2D5B8E%2D4F95%2D8DF7%2D47E076627D12%40sessionmgr6+812C&_us=SLsrc+ext+or+Date+034D&_usmtl=ftv+True+137E&_uso=hd+False+db%5B0+%2Daph+1BEE&fi=aph_2752232_AN&lpdf=true&pdfs=&tn=&tp=PC&es=cs%5Fclient%2Easp%3FT%3DP%26P%3DAN%26K%3D2752232%26rn%3D1%26db%3Daph%26is%3D0028%2D0836%26sc%3D%26S%3D%26D%3Daph%26title%3DNature%26year%3D2000%26bk%3DS&fn=1&rn=1&bk=S&EBSCOContent=ZWJjY8bb43ePprRruevra6Gmr4GPprOFoaa5fKaWxpjDpfG60uGtuNDf7XnU3u6+4wAA&an=2752232&db=aph& here]<br />
<br />
==20 June==<br />
Hayato presents an article on producing the luciferace-magnetic particle complex.<br />
<br />
6pm Walter Hall<br />
<br />
==16 May==<br />
This is the last journal club until the summer. This week will be a meeting to discuss the lab and the first few weeks of the summer. In Walter Hall, 80 Waterman, 6-7pm<br />
<br />
==9 May==<br />
<br />
Journal Club is in Walter Hall, 80 Waterman<br />
<br />
Hiyato to present: Nanoparticle labels in immunosensing using optical detection methods<br />
<br />
Matthias Seydack<br />
<br />
==2 May==<br />
<br />
[http://openwetware.org/wiki/Sriram_Kosuri Sri Kosuri] from MIT to come and talk about his paper from the previous week.<br />
<br />
==25 April==<br />
<br />
John to present, + overview of Biobricks<br />
<br />
[http://www.nature.com/msb/journal/v1/n1/full/msb4100025.html Refactoring bacteriophage T7]<br />
Leon Y Chan1,a, Sriram Kosuri2,a and Drew Endy2<br />
<br />
==18 April==<br />
<br />
Kara and Jesse<br />
<br />
http://www.nature.com/nbt/journal/v23/n3/abs/nbt1069.html<br />
<br />
Programmable ligand-controlled riboregulators of eukaryotic gene expression.<br />
<br />
Bayer TS, Smolke CD.<br />
<br />
==11 April==<br />
Annie and Angela will present the article: "Design of artificial cell–cell communication using gene and metabolic networks".<br />
<br />
The paper can be found at: http://www.pnas.org/cgi/content/short/101/8/2299<br />
<br />
Thomas Bulter, Sun-Gu Lee, Wilson WaiChun Wong, Eileen Fung, Michael R. Conner, and James C. Liao. 2004. Design of artificial cell-cell communication using gene and metabolic networks. PNAS. 101(8): 2299-2304. (Quorum sensor using acetate signal).<br />
<br />
==4 April==<br />
Brendan and Peter will be presenting the article entitled "Engineering a<br />
mevalonate pathway in Escherichia coli for production of terpenoids".<br />
<br />
Vincent J J Martin, Douglas J Pitera1, Sydnor T Withers1, Jack D Newman & Jay D Keasling. "Engineering a<br />
mevalonate pathway in Escherichia coli for production of terpenoids." Nature Biotechnology 21, 796 - 802 (2003).<br />
<br />
It can be found online at http://www.nature.com/nbt/journal/v21/n7/abs/nbt833.html<br />
<Br><br />
<br />
==21 March==<br />
Megan and Victoria will present the article handed out in last meeting. Article is entitled: "Construction of a genetic toggle switch in Escherichia coli" <br><br />
<br />
Timothy S. Gardner, Charles R. Cantor, and James J. Collins. 2000. Construction of a genetic toggle switch in Escherichia coli. Nature. Vol. 403. 339 - 342. (Bistable gene regulatory network, toggled by transient chemical or thermal induction, to serve as cellular memory) [http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html Download the paper here]<br />
<br />
==14th March==<br />
John to give overview of last year's competition and to hand out readings<br />
[http://www.nature.com/nature/journal/v438/n7067/abs/nature04405.html Download paper here]<br />
<br />
==November 29, 2006==<br />
Hayato will be presenting a paper on DNA autorepair. Wednesday at 5:15pm, location to be announced.<br><br />
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=17006450 Link to abstract]<br><br />
[http://www.nature.com/nature/journal/v443/n7111/pdf/443517b.pdf Link to paper]</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:iGEM_portal_2006Brown:iGEM portal 20062006-11-28T05:04:31Z<p>Agao: </p>
<hr />
<div>{{Brown navigation bar}}<br />
[[Image:TeamPhotoBrown3.jpg|750px|Some of the Brown iGEM Team]]<br />
<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0; width: 750px; color: #000000; background-color: #ffffff;"<br />
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<br />
<h3>[[Brown:Projects | See our 2006 iGEM Jamboree entry here]] </h3><br />
<br />
<h3>Welcome</h3><br />
Welcome to the Brown [http://icampus.mit.edu/projects/iGem.shtml International Genetically Engineered Machine (iGEM)] Team Wiki. If you are interested in taking part in iGEM 2007, then please [[Brown:2007 | come along to our meetings or join our mailing list.]]<br />
<br />
<h3>Team news </h3><br />
11/29/06- Next journal club meeting will be held on Wednesday November 29 at 5:15, location to be announced. Hayato will be presenting a paper on DNA repair <br><br />
11/05/06 - Get info on the [[Brown:2007 | Brown iGEM team 2007]] <br><br />
11/02/06 - We're presenting our work this Saturday, at the [http://www.igem2006.com iGEM Jamboree at MIT] <br><br />
10/22/06 - Abstract accepted for poster presentation at the BioSysBio Conference in the UK! <br><br />
09/26/06 - The team recently visited Pfizer in Groton, CT <br><br />
7/18/06 - Brendan has added details and [[Brown:Projects|the freeze-tag project]] <br><br />
<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>Team roles</h3><br />
<br />
Team Leaders: [[user:jamielemon|Jamie L.]], [[user:anniegao|Annie]]<br><br />
Treasurer: [[user:anniegao|Annie]]<br><br />
Secretary: [[user:Victorialattanzi|Victoria]]<br><br />
Journal Club Coordinator: [[user:petergoldstein|Peter]] <br><br />
Faculty Liaison: [[user:azeemkaka| Azeem]]<br><br />
Industry Liaison: [[user:victorialattanzi|Victoria]]<br> <br />
Outreach: [[user: meganschmidt|Megan]]<br> <br />
Future Planning: [[user:Jasonlohmueller|Jason]] <br> <br />
Chemical DB Manager: [[user:petergoldstein|Peter]] <br><br />
Guest Speaker Organizer: [[user:johncumbers|John]] <br><br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>[[Brown:Todo list |Todo list]]</h3><br />
<h3>[[Brown:Lab Protocols| Lab Protocols]] </h3><br />
<br />
<h3>[[Brown:Weekly Meetings|Weekly Meetings]]</h3><br />
<br />
<h3>[[Brown:Equipment needed|Equipment needed]]</h3><br />
<br />
<h3>[[Brown:Summer workshops|Summer Workshops]]</h3><br />
<br />
<h3>[[Brown:Calendar pages|Calendar Pages]]</h3><br />
<br />
<h3>[[Brown:Projects|Projects]]</h3><br />
<br />
<h3>[[Brown:Journal_club:Synthetic_biology_journal_club|Journal Club]]</h3><br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>About Us</h3><br />
#[[Brown:Lab Members | Faculty Advisors]]<br />
#[[Brown:Lab Members | Team Members]]<br />
#[[Brown:Industry Affiliates | Industry Affiliates]]<br />
<br />
Our lab is in J Walter Wilson, Rm 228, call 401 523 8190<br />
<br />
#[[Brown:2007 | Brown iGEM team 2007]]<br />
<br />
<br />
__NOTOC__</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Journal_club:Synthetic_biology_journal_clubBrown:Journal club:Synthetic biology journal club2006-11-28T05:02:33Z<p>Agao: /* November 29, 2006 */</p>
<hr />
<div>{{Brown navigation bar}} <br><br />
==25 July==<br />
Annie to present article titled "Synthetic protein-protein interatction domains created by shuffling Cys2His2 zinc-fingers".<br><br />
[http://www.nature.com/msb/journal/v2/n1/full/msb4100053.html Link to Article]<br />
<br />
==18 July==<br />
<br />
Jason to present on Article titled ''Environmentally Controlled Invasion of Cancer<br />
Cells by Engineered Bacteria''. <br><br />
<br />
[http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WK7-4HJRXGD-4-3&_cdi=6899&_user=489286&_orig=search&_coverDate=01%2F27%2F2006&_qd=1&_sk=996449995&view=c&wchp=dGLbVzb-zSkWA&md5=d973cfd361022b29e92d1ccc48c28b92&ie=/sdarticle.pdf PDF of Article]<br />
<br />
=='''Archive'''==<br />
==11 July==<br />
Brendan to present on Article titled ''Ultrasensitivity and Nosie Propagation in a Synthetic Transcriptional Cascade''. <br><br />
<br />
[http://www.pnas.org/cgi/reprint/102/10/3581 PDF of Article]<br />
<br />
==5 July==<br />
Peter presents an article about transcriptional regulators used to form an oscillator. The article is [http://sas.epnet.com/externalframe.asp?tb=0&_ug=sid+22B5129D%2D5B8E%2D4F95%2D8DF7%2D47E076627D12%40sessionmgr6+812C&_us=SLsrc+ext+or+Date+034D&_usmtl=ftv+True+137E&_uso=hd+False+db%5B0+%2Daph+1BEE&fi=aph_2752232_AN&lpdf=true&pdfs=&tn=&tp=PC&es=cs%5Fclient%2Easp%3FT%3DP%26P%3DAN%26K%3D2752232%26rn%3D1%26db%3Daph%26is%3D0028%2D0836%26sc%3D%26S%3D%26D%3Daph%26title%3DNature%26year%3D2000%26bk%3DS&fn=1&rn=1&bk=S&EBSCOContent=ZWJjY8bb43ePprRruevra6Gmr4GPprOFoaa5fKaWxpjDpfG60uGtuNDf7XnU3u6+4wAA&an=2752232&db=aph& here]<br />
<br />
==20 June==<br />
Hayato presents an article on producing the luciferace-magnetic particle complex.<br />
<br />
6pm Walter Hall<br />
<br />
==16 May==<br />
This is the last journal club until the summer. This week will be a meeting to discuss the lab and the first few weeks of the summer. In Walter Hall, 80 Waterman, 6-7pm<br />
<br />
==9 May==<br />
<br />
Journal Club is in Walter Hall, 80 Waterman<br />
<br />
Hiyato to present: Nanoparticle labels in immunosensing using optical detection methods<br />
<br />
Matthias Seydack<br />
<br />
==2 May==<br />
<br />
[http://openwetware.org/wiki/Sriram_Kosuri Sri Kosuri] from MIT to come and talk about his paper from the previous week.<br />
<br />
==25 April==<br />
<br />
John to present, + overview of Biobricks<br />
<br />
[http://www.nature.com/msb/journal/v1/n1/full/msb4100025.html Refactoring bacteriophage T7]<br />
Leon Y Chan1,a, Sriram Kosuri2,a and Drew Endy2<br />
<br />
==18 April==<br />
<br />
Kara and Jesse<br />
<br />
http://www.nature.com/nbt/journal/v23/n3/abs/nbt1069.html<br />
<br />
Programmable ligand-controlled riboregulators of eukaryotic gene expression.<br />
<br />
Bayer TS, Smolke CD.<br />
<br />
==11 April==<br />
Annie and Angela will present the article: "Design of artificial cell–cell communication using gene and metabolic networks".<br />
<br />
The paper can be found at: http://www.pnas.org/cgi/content/short/101/8/2299<br />
<br />
Thomas Bulter, Sun-Gu Lee, Wilson WaiChun Wong, Eileen Fung, Michael R. Conner, and James C. Liao. 2004. Design of artificial cell-cell communication using gene and metabolic networks. PNAS. 101(8): 2299-2304. (Quorum sensor using acetate signal).<br />
<br />
==4 April==<br />
Brendan and Peter will be presenting the article entitled "Engineering a<br />
mevalonate pathway in Escherichia coli for production of terpenoids".<br />
<br />
Vincent J J Martin, Douglas J Pitera1, Sydnor T Withers1, Jack D Newman & Jay D Keasling. "Engineering a<br />
mevalonate pathway in Escherichia coli for production of terpenoids." Nature Biotechnology 21, 796 - 802 (2003).<br />
<br />
It can be found online at http://www.nature.com/nbt/journal/v21/n7/abs/nbt833.html<br />
<Br><br />
<br />
==21 March==<br />
Megan and Victoria will present the article handed out in last meeting. Article is entitled: "Construction of a genetic toggle switch in Escherichia coli" <br><br />
<br />
Timothy S. Gardner, Charles R. Cantor, and James J. Collins. 2000. Construction of a genetic toggle switch in Escherichia coli. Nature. Vol. 403. 339 - 342. (Bistable gene regulatory network, toggled by transient chemical or thermal induction, to serve as cellular memory) [http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html Download the paper here]<br />
<br />
==14th March==<br />
John to give overview of last year's competition and to hand out readings<br />
[http://www.nature.com/nature/journal/v438/n7067/abs/nature04405.html Download paper here]<br />
<br />
==November 29, 2006==<br />
Hayato will be presenting a paper on DNA autorepair. Wednesday at 5:15pm, location to be announced.<br><br />
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=17006450 Link to abstract]<br><br />
[http://www.nature.com/nature/journal/v443/n7111/pdf/443517a.pdf Link to paper]</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Journal_club:Synthetic_biology_journal_clubBrown:Journal club:Synthetic biology journal club2006-11-28T05:02:17Z<p>Agao: /* 14th March */</p>
<hr />
<div>{{Brown navigation bar}} <br><br />
==25 July==<br />
Annie to present article titled "Synthetic protein-protein interatction domains created by shuffling Cys2His2 zinc-fingers".<br><br />
[http://www.nature.com/msb/journal/v2/n1/full/msb4100053.html Link to Article]<br />
<br />
==18 July==<br />
<br />
Jason to present on Article titled ''Environmentally Controlled Invasion of Cancer<br />
Cells by Engineered Bacteria''. <br><br />
<br />
[http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WK7-4HJRXGD-4-3&_cdi=6899&_user=489286&_orig=search&_coverDate=01%2F27%2F2006&_qd=1&_sk=996449995&view=c&wchp=dGLbVzb-zSkWA&md5=d973cfd361022b29e92d1ccc48c28b92&ie=/sdarticle.pdf PDF of Article]<br />
<br />
=='''Archive'''==<br />
==11 July==<br />
Brendan to present on Article titled ''Ultrasensitivity and Nosie Propagation in a Synthetic Transcriptional Cascade''. <br><br />
<br />
[http://www.pnas.org/cgi/reprint/102/10/3581 PDF of Article]<br />
<br />
==5 July==<br />
Peter presents an article about transcriptional regulators used to form an oscillator. The article is [http://sas.epnet.com/externalframe.asp?tb=0&_ug=sid+22B5129D%2D5B8E%2D4F95%2D8DF7%2D47E076627D12%40sessionmgr6+812C&_us=SLsrc+ext+or+Date+034D&_usmtl=ftv+True+137E&_uso=hd+False+db%5B0+%2Daph+1BEE&fi=aph_2752232_AN&lpdf=true&pdfs=&tn=&tp=PC&es=cs%5Fclient%2Easp%3FT%3DP%26P%3DAN%26K%3D2752232%26rn%3D1%26db%3Daph%26is%3D0028%2D0836%26sc%3D%26S%3D%26D%3Daph%26title%3DNature%26year%3D2000%26bk%3DS&fn=1&rn=1&bk=S&EBSCOContent=ZWJjY8bb43ePprRruevra6Gmr4GPprOFoaa5fKaWxpjDpfG60uGtuNDf7XnU3u6+4wAA&an=2752232&db=aph& here]<br />
<br />
==20 June==<br />
Hayato presents an article on producing the luciferace-magnetic particle complex.<br />
<br />
6pm Walter Hall<br />
<br />
==16 May==<br />
This is the last journal club until the summer. This week will be a meeting to discuss the lab and the first few weeks of the summer. In Walter Hall, 80 Waterman, 6-7pm<br />
<br />
==9 May==<br />
<br />
Journal Club is in Walter Hall, 80 Waterman<br />
<br />
Hiyato to present: Nanoparticle labels in immunosensing using optical detection methods<br />
<br />
Matthias Seydack<br />
<br />
==2 May==<br />
<br />
[http://openwetware.org/wiki/Sriram_Kosuri Sri Kosuri] from MIT to come and talk about his paper from the previous week.<br />
<br />
==25 April==<br />
<br />
John to present, + overview of Biobricks<br />
<br />
[http://www.nature.com/msb/journal/v1/n1/full/msb4100025.html Refactoring bacteriophage T7]<br />
Leon Y Chan1,a, Sriram Kosuri2,a and Drew Endy2<br />
<br />
==18 April==<br />
<br />
Kara and Jesse<br />
<br />
http://www.nature.com/nbt/journal/v23/n3/abs/nbt1069.html<br />
<br />
Programmable ligand-controlled riboregulators of eukaryotic gene expression.<br />
<br />
Bayer TS, Smolke CD.<br />
<br />
==11 April==<br />
Annie and Angela will present the article: "Design of artificial cell–cell communication using gene and metabolic networks".<br />
<br />
The paper can be found at: http://www.pnas.org/cgi/content/short/101/8/2299<br />
<br />
Thomas Bulter, Sun-Gu Lee, Wilson WaiChun Wong, Eileen Fung, Michael R. Conner, and James C. Liao. 2004. Design of artificial cell-cell communication using gene and metabolic networks. PNAS. 101(8): 2299-2304. (Quorum sensor using acetate signal).<br />
<br />
==4 April==<br />
Brendan and Peter will be presenting the article entitled "Engineering a<br />
mevalonate pathway in Escherichia coli for production of terpenoids".<br />
<br />
Vincent J J Martin, Douglas J Pitera1, Sydnor T Withers1, Jack D Newman & Jay D Keasling. "Engineering a<br />
mevalonate pathway in Escherichia coli for production of terpenoids." Nature Biotechnology 21, 796 - 802 (2003).<br />
<br />
It can be found online at http://www.nature.com/nbt/journal/v21/n7/abs/nbt833.html<br />
<Br><br />
<br />
==21 March==<br />
Megan and Victoria will present the article handed out in last meeting. Article is entitled: "Construction of a genetic toggle switch in Escherichia coli" <br><br />
<br />
Timothy S. Gardner, Charles R. Cantor, and James J. Collins. 2000. Construction of a genetic toggle switch in Escherichia coli. Nature. Vol. 403. 339 - 342. (Bistable gene regulatory network, toggled by transient chemical or thermal induction, to serve as cellular memory) [http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html Download the paper here]<br />
<br />
==14th March==<br />
John to give overview of last year's competition and to hand out readings<br />
[http://www.nature.com/nature/journal/v438/n7067/abs/nature04405.html Download paper here]<br />
<br />
==November 29, 2006==<br />
Hayato will be presenting a paper on DNA autorepair. Wednesday at 5:15pm, location to be announced.<br />
[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=17006450 Link to abstract]<br />
[http://www.nature.com/nature/journal/v443/n7111/pdf/443517a.pdf Link to paper]</div>Agaohttp://2006.igem.org/wiki/index.php/%27Freeze_Machine%27'Freeze Machine'2006-07-07T14:47:27Z<p>Agao: </p>
<hr />
<div>{{Brown navigation bar}}<br />
==General Overview==<br />
A promoter that is regulated by LacI will promote the production of LasI, MotB, and cI. This will subsequently inhibit the production of CFP and LasR. In the presence of LacI, however, MotB, LasI, and cI will not be produced. CFP will therefore be produced along with LasR and LacI. This results in the "freezing" of the cell.</div>Agaohttp://2006.igem.org/wiki/index.php/%27Freeze_Machine%27'Freeze Machine'2006-07-07T14:46:49Z<p>Agao: </p>
<hr />
<div>[[Brown:Projects|Back]]<br />
==General Overview==<br />
A promoter that is regulated by LacI will promote the production of LasI, MotB, and cI. This will subsequently inhibit the production of CFP and LasR. In the presence of LacI, however, MotB, LasI, and cI will not be produced. CFP will therefore be produced along with LasR and LacI. This results in the "freezing" of the cell.</div>Agaohttp://2006.igem.org/wiki/index.php/%27Freeze_Machine%27'Freeze Machine'2006-07-07T14:45:55Z<p>Agao: </p>
<hr />
<div>==General Overview==<br />
A promoter that is regulated by LacI will promote the production of LasI, MotB, and cI. This will subsequently inhibit the production of CFP and LasR. In the presence of LacI, however, MotB, LasI, and cI will not be produced. CFP will therefore be produced along with LasR and LacI. This results in the "freezing" of the cell.</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Todo_listBrown:Todo list2006-07-07T14:30:52Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br />
{{Brown navigation bar}}<br />
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|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #CC6600;" |<br />
<br />
<h5>Annie</h5> <br />
#Freeze-Machine<br />
<br />
<h5>Sheldon</h5><br />
- No tasks for now<br />
<br />
<h5>John</h5><br />
#Lead<br />
#Wiki<br />
#Funding<br />
#Department fundraising<br />
#Future planning<br />
<br />
<h5>Ana</h5><br />
#Magnetic bacteria<br />
#Contact Sheldon re: alumni magazine<br />
<br />
<h5>Jamie L</h5><br />
#Alumni DB (biotech)<br />
#Contact entrepeneurship program<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h5>Megan</h5> <br />
<br />
#AHL Sensor<br />
<br />
<h5>Victoria</h5><br />
#Pfizer wish list<br />
<br />
<h5>Jamie G </h5><br />
#Magnetic bacteria<br />
<br />
<h5>Azeem</h5><br />
#Research<br />
#Modeling<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h5>Brendan</h5><br />
#PR<br />
#CS<br />
#MCB<br />
#Sorin (contact)<br />
#Goldman Sachs<br />
<br />
<h5>Peter</h5><br />
#Wiki<br />
#CS<br />
#Redo todo list<br />
<br />
<h5>Hiyato </h5><br />
#Free radical reporter<br />
<br />
<h5>Jason</h5><br />
#Research<br />
<br />
|}<br />
<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #CC6600;" |<br />
<br />
<h3>iGEM</h3> <br />
#org lunch<br />
#idea update<br />
#bac magnetic update<br />
#current students list in order<br />
#decide on faculty board mentors, split up, decide meeting time<br />
#next week's JC<br />
#tech transfer "IP feasibility report"<br />
#Amgen sponsorship<br />
#ask advisors grads to be involved<br />
# small photo of each team member<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h3>Funding</h3><br />
#find alumns at cisco <br />
#liase with alumn mag<br />
#Azeem is writing a letter to send to Amgen (via Barrat) and Novartis (via Karen), JOhn to write to Invitrogen via Sue<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h3>Other tasks</h3><br />
#redo todo list<br />
#Arrange health and safety course, see Karen's mail<br />
#define faculty mentoring team<br />
#summer plan, monthly<br />
#summer plan weekly<br />
#summer plan daily<br />
#courses and workshops to prepare<br />
#equipment needed<br />
<br />
<h3>Publicity</h3><br />
#add tasks<br />
#add tasks<br />
#add tasks<br />
|}</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Todo_listBrown:Todo list2006-07-07T14:29:47Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br />
{{Brown navigation bar}}<br />
__NOTOC__<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #CC6600;" |<br />
<br />
<h5>Annie</h5> <br />
#Freeze-Machine<br />
<br />
<h5>Sheldon</h5><br />
- No tasks for now<br />
<br />
<h5>John</h5><br />
#Lead<br />
#Wiki<br />
#Funding<br />
#Department fundraising<br />
#Future planning<br />
<br />
<h5>Ana</h5><br />
#Magnetic bacteria<br />
#Contact Sheldon re: alumni magazine<br />
<br />
<h5>Jamie L</h5><br />
#Alumni DB (biotech)<br />
#Contact entrepeneurship program<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h5>Megan</h5> <br />
<br />
#AHL Sensor<br />
<br />
<h5>Victoria</h5><br />
#Pfizer wish list<br />
<br />
<h5>Jamie G </h5><br />
#Magnetic bacteria<br />
<br />
<h5>Azeem</h5><br />
#Research<br />
#Modeling<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h5>Brendan</h5><br />
#PR<br />
#CS<br />
#MCB<br />
#Sorin (contact)<br />
#Goldman Sachs<br />
<br />
<h5>Peter</h5><br />
#Wiki<br />
#CS<br />
#Redo todo list<br />
<br />
<h5>Hiyato </h5><br />
#Free radical reporter<br />
<br />
<h5>Jason</h5><br />
#Free radical reporter<br />
#Equipment needed<br />
<br />
|}<br />
<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #CC6600;" |<br />
<br />
<h3>iGEM</h3> <br />
#org lunch<br />
#idea update<br />
#bac magnetic update<br />
#current students list in order<br />
#decide on faculty board mentors, split up, decide meeting time<br />
#next week's JC<br />
#tech transfer "IP feasibility report"<br />
#Amgen sponsorship<br />
#ask advisors grads to be involved<br />
# small photo of each team member<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h3>Funding</h3><br />
#find alumns at cisco <br />
#liase with alumn mag<br />
#Azeem is writing a letter to send to Amgen (via Barrat) and Novartis (via Karen), JOhn to write to Invitrogen via Sue<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h3>Other tasks</h3><br />
#redo todo list<br />
#Arrange health and safety course, see Karen's mail<br />
#define faculty mentoring team<br />
#summer plan, monthly<br />
#summer plan weekly<br />
#summer plan daily<br />
#courses and workshops to prepare<br />
#equipment needed<br />
<br />
<h3>Publicity</h3><br />
#add tasks<br />
#add tasks<br />
#add tasks<br />
|}</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Todo_listBrown:Todo list2006-07-07T14:29:25Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br />
{{Brown navigation bar}}<br />
__NOTOC__<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #CC6600;" |<br />
<br />
<h5>Annie</h5> <br />
#Research<br />
<br />
<h5>Sheldon</h5><br />
- No tasks for now<br />
<br />
<h5>John</h5><br />
#Lead<br />
#Wiki<br />
#Funding<br />
#Department fundraising<br />
#Future planning<br />
<br />
<h5>Ana</h5><br />
#Magnetic bacteria<br />
#Contact Sheldon re: alumni magazine<br />
<br />
<h5>Jamie L</h5><br />
#Alumni DB (biotech)<br />
#Contact entrepeneurship program<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h5>Megan</h5> <br />
<br />
#AHL Sensor<br />
<br />
<h5>Victoria</h5><br />
#Pfizer wish list<br />
<br />
<h5>Jamie G </h5><br />
#Magnetic bacteria<br />
<br />
<h5>Azeem</h5><br />
#Research<br />
#Modeling<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h5>Brendan</h5><br />
#PR<br />
#CS<br />
#MCB<br />
#Sorin (contact)<br />
#Goldman Sachs<br />
<br />
<h5>Peter</h5><br />
#Wiki<br />
#CS<br />
#Redo todo list<br />
<br />
<h5>Hiyato </h5><br />
#Free radical reporter<br />
<br />
<h5>Jason</h5><br />
#Free radical reporter<br />
#Equipment needed<br />
<br />
|}<br />
<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #CC6600;" |<br />
<br />
<h3>iGEM</h3> <br />
#org lunch<br />
#idea update<br />
#bac magnetic update<br />
#current students list in order<br />
#decide on faculty board mentors, split up, decide meeting time<br />
#next week's JC<br />
#tech transfer "IP feasibility report"<br />
#Amgen sponsorship<br />
#ask advisors grads to be involved<br />
# small photo of each team member<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h3>Funding</h3><br />
#find alumns at cisco <br />
#liase with alumn mag<br />
#Azeem is writing a letter to send to Amgen (via Barrat) and Novartis (via Karen), JOhn to write to Invitrogen via Sue<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h3>Other tasks</h3><br />
#redo todo list<br />
#Arrange health and safety course, see Karen's mail<br />
#define faculty mentoring team<br />
#summer plan, monthly<br />
#summer plan weekly<br />
#summer plan daily<br />
#courses and workshops to prepare<br />
#equipment needed<br />
<br />
<h3>Publicity</h3><br />
#add tasks<br />
#add tasks<br />
#add tasks<br />
|}</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Todo_listBrown:Todo list2006-07-07T14:29:01Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br />
{{Brown navigation bar}}<br />
__NOTOC__<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #CC6600;" |<br />
<br />
<h5>Annie</h5> <br />
#Research<br />
<br />
<h5>Sheldon</h5><br />
- No tasks for now<br />
<br />
<h5>John</h5><br />
#Lead<br />
#Wiki<br />
#Funding<br />
#Department fundraising<br />
#Future planning<br />
<br />
<h5>Ana</h5><br />
#Magnetic bacteria<br />
#Contact Sheldon re: alumni magazine<br />
<br />
<h5>Jamie L</h5><br />
#Alumni DB (biotech)<br />
#Contact entrepeneurship program<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h5>Megan</h5> <br />
<br />
#AHL Sensor<br />
<br />
<h5>Victoria</h5><br />
#Pfizer wish list<br />
<br />
<h5>Jamie G </h5><br />
#Magnetic bacteria<br />
<br />
<h5>Azeem</h5><br />
#Research<br />
#Cell counter<br />
#Monthy planning<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<br />
<br />
<br />
<br />
<h5>Brendan</h5><br />
#PR<br />
#CS<br />
#MCB<br />
#Sorin (contact)<br />
#Goldman Sachs<br />
<br />
<h5>Peter</h5><br />
#Wiki<br />
#CS<br />
#Redo todo list<br />
<br />
<h5>Hiyato </h5><br />
#Free radical reporter<br />
<br />
<h5>Jason</h5><br />
#Free radical reporter<br />
#Equipment needed<br />
<br />
|}<br />
<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #CC6600;" |<br />
<br />
<h3>iGEM</h3> <br />
#org lunch<br />
#idea update<br />
#bac magnetic update<br />
#current students list in order<br />
#decide on faculty board mentors, split up, decide meeting time<br />
#next week's JC<br />
#tech transfer "IP feasibility report"<br />
#Amgen sponsorship<br />
#ask advisors grads to be involved<br />
# small photo of each team member<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h3>Funding</h3><br />
#find alumns at cisco <br />
#liase with alumn mag<br />
#Azeem is writing a letter to send to Amgen (via Barrat) and Novartis (via Karen), JOhn to write to Invitrogen via Sue<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h3>Other tasks</h3><br />
#redo todo list<br />
#Arrange health and safety course, see Karen's mail<br />
#define faculty mentoring team<br />
#summer plan, monthly<br />
#summer plan weekly<br />
#summer plan daily<br />
#courses and workshops to prepare<br />
#equipment needed<br />
<br />
<h3>Publicity</h3><br />
#add tasks<br />
#add tasks<br />
#add tasks<br />
|}</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Todo_listBrown:Todo list2006-07-07T14:28:48Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br />
{{Brown navigation bar}}<br />
__NOTOC__<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #CC6600;" |<br />
<br />
<h5>Annie</h5> <br />
#Research<br />
<br />
<h5>Sheldon</h5><br />
- No tasks for now<br />
<br />
<h5>John</h5><br />
#Lead<br />
#Wiki<br />
#Funding<br />
#Department fundraising<br />
#Future planning<br />
<br />
<h5>Ana</h5><br />
#Magnetic bacteria<br />
#Contact Sheldon re: alumni magazine<br />
<br />
<h5>Jamie L</h5><br />
#Alumni DB (biotech)<br />
#Contact entrepeneurship program<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h5>Megan</h5> <br />
<br />
#AHL Sensor<br />
<br />
<h5>Victoria</h5><br />
#Pfizer wish list<br />
#Faculty mentoring group (when to meet)<br />
#Alumni database (biotech)<br />
#Contact entrepeneurship program<br />
<br />
<br />
<br />
<h5>Jamie G </h5><br />
#Magnetic bacteria<br />
<br />
<h5>Azeem</h5><br />
#Research<br />
#Cell counter<br />
#Monthy planning<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<br />
<br />
<br />
<br />
<h5>Brendan</h5><br />
#PR<br />
#CS<br />
#MCB<br />
#Sorin (contact)<br />
#Goldman Sachs<br />
<br />
<h5>Peter</h5><br />
#Wiki<br />
#CS<br />
#Redo todo list<br />
<br />
<h5>Hiyato </h5><br />
#Free radical reporter<br />
<br />
<h5>Jason</h5><br />
#Free radical reporter<br />
#Equipment needed<br />
<br />
|}<br />
<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #CC6600;" |<br />
<br />
<h3>iGEM</h3> <br />
#org lunch<br />
#idea update<br />
#bac magnetic update<br />
#current students list in order<br />
#decide on faculty board mentors, split up, decide meeting time<br />
#next week's JC<br />
#tech transfer "IP feasibility report"<br />
#Amgen sponsorship<br />
#ask advisors grads to be involved<br />
# small photo of each team member<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h3>Funding</h3><br />
#find alumns at cisco <br />
#liase with alumn mag<br />
#Azeem is writing a letter to send to Amgen (via Barrat) and Novartis (via Karen), JOhn to write to Invitrogen via Sue<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h3>Other tasks</h3><br />
#redo todo list<br />
#Arrange health and safety course, see Karen's mail<br />
#define faculty mentoring team<br />
#summer plan, monthly<br />
#summer plan weekly<br />
#summer plan daily<br />
#courses and workshops to prepare<br />
#equipment needed<br />
<br />
<h3>Publicity</h3><br />
#add tasks<br />
#add tasks<br />
#add tasks<br />
|}</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Todo_listBrown:Todo list2006-07-07T14:28:33Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br />
{{Brown navigation bar}}<br />
__NOTOC__<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #CC6600;" |<br />
<br />
<h5>Annie</h5> <br />
#Research<br />
<br />
<h5>Sheldon</h5><br />
- No tasks for now<br />
<br />
<h5>John</h5><br />
#Lead<br />
#Wiki<br />
#Funding<br />
#Department fundraising<br />
#Future planning<br />
<br />
<h5>Ana</h5><br />
#Magnetic bacteria<br />
#Contact Sheldon re: alumni magazine<br />
<br />
<h5>Jamie L</h5><br />
#Alumni DB (biotech)<br />
#Contact entrepeneurship program<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h5>Megan</h5> <br />
<br />
#Future planning<br />
#Pfizer meeting<br />
# thank you notes to departments and president's office<br />
<br />
<h5>Victoria</h5><br />
#Pfizer wish list<br />
#Faculty mentoring group (when to meet)<br />
#Alumni database (biotech)<br />
#Contact entrepeneurship program<br />
<br />
<br />
<br />
<h5>Jamie G </h5><br />
#Magnetic bacteria<br />
<br />
<h5>Azeem</h5><br />
#Research<br />
#Cell counter<br />
#Monthy planning<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<br />
<br />
<br />
<br />
<h5>Brendan</h5><br />
#PR<br />
#CS<br />
#MCB<br />
#Sorin (contact)<br />
#Goldman Sachs<br />
<br />
<h5>Peter</h5><br />
#Wiki<br />
#CS<br />
#Redo todo list<br />
<br />
<h5>Hiyato </h5><br />
#Free radical reporter<br />
<br />
<h5>Jason</h5><br />
#Free radical reporter<br />
#Equipment needed<br />
<br />
|}<br />
<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #CC6600;" |<br />
<br />
<h3>iGEM</h3> <br />
#org lunch<br />
#idea update<br />
#bac magnetic update<br />
#current students list in order<br />
#decide on faculty board mentors, split up, decide meeting time<br />
#next week's JC<br />
#tech transfer "IP feasibility report"<br />
#Amgen sponsorship<br />
#ask advisors grads to be involved<br />
# small photo of each team member<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h3>Funding</h3><br />
#find alumns at cisco <br />
#liase with alumn mag<br />
#Azeem is writing a letter to send to Amgen (via Barrat) and Novartis (via Karen), JOhn to write to Invitrogen via Sue<br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #666666;" |<br />
<br />
<h3>Other tasks</h3><br />
#redo todo list<br />
#Arrange health and safety course, see Karen's mail<br />
#define faculty mentoring team<br />
#summer plan, monthly<br />
#summer plan weekly<br />
#summer plan daily<br />
#courses and workshops to prepare<br />
#equipment needed<br />
<br />
<h3>Publicity</h3><br />
#add tasks<br />
#add tasks<br />
#add tasks<br />
|}</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:iGEM_portal_2006Brown:iGEM portal 20062006-07-07T14:28:06Z<p>Agao: </p>
<hr />
<div>{{Brown navigation bar}}<br />
<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0; width: 750px; color: #000000; background-color: #ffffff;"<br />
| width=717px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<h3>Welcome</h3><br />
Welcome to the Brown [http://icampus.mit.edu/projects/iGem.shtml International Genetically Engineered Machine (iGEM)] Team Wiki. Our goal is to participate in the iGEM Competition for the summer of 2006. Feel free to explore our website or [[Brown:Contact | contact us]], we are constantly updating it with the latest developments in the project. <br />
<br />
<h3>Team news </h3><br />
6/18/06 - Funded students - remember you need to add [[User:Johncumbers| a weekly report]] to your user page <br><br />
6/18/06 - Updated [[Brown:Calendar pages |calendar page]], now all info on single page <br><br />
4/22/06 - Please pick up some tasks from the new [[Brown:Todo list | To do list]] <br><br />
3/29/06 - Updated [[Brown:Roles/future planning| Future planning]] section <br><br />
3/29/06 - Please make sure you have signed yourself up for the [[Brown:Journal club:Synthetic biology journal club |journal club]] <br><br />
3/29/06 - If you are in charge of one of the roles listed below, please update your section with your ideas and plans<br />
<br />
|}<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>Team roles</h3><br />
<br />
Team Leaders: [[user:jamielemon|Jamie L.]], [[user:anniegao|Annie]]<br><br />
Fundraising: <br><br />
Secretary: [[user:Victorialattanzi|Victoria]]<br><br />
Journal Club Coordinator: [[user:petergoldstein|Peter]] <br><br />
Faculty Liason: [[user:azeemkaka| Azeem]]<br><br />
Industry Liason: <br> <br />
Outreach: <br> <br />
[[Brown:Roles/future planning| Future planning]]: [[user:johncumbers|John]] <br> <br />
Chemical DB Manager: [[user:petergoldstein|Peter]] <br><br />
Guest Speaker Organizer: [[user:johncumbers|John]] <br><br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>[[Brown:Todo list |Todo list]]</h3><br />
<h3>[[Brown:Lab Protocols| Lab Protocols]] </h3><br />
<h3>[[Brown:Next Meeting Agenda |Next Meeting Agenda ]]</h3><br />
<br />
<h3>[[Brown:Equipment needed|Equipment needed]]</h3><br />
<br />
<h3>[[Brown:Summer workshops|Summer Workshops]]</h3><br />
<br />
<h3>[[Brown:Calendar pages|Calendar Pages]]</h3><br />
<br />
<h3>[[Brown:Projects |Projects]]</h3><br />
<br />
<h3>Research</h3><br />
#[[Brown:Journal_club:Synthetic_biology_journal_club|Journal Club]]<br />
#[http://www.cumbers.com/igem Idea factory] <br><br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>About Us</h3><br />
#[[Brown:Lab Members | Faculty Advisors]]<br />
#[[Brown:Lab Members | Team Members]]<br />
#[[Brown:Industry Affiliates | Industry Affiliates]]<br />
#[[Brown:March142006 | Meeting minutes]]<br />
<br />
Our lab is in J Walter Wilson, Rm 228, call 401 523 8190<br />
<br />
__NOTOC__</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:iGEM_portal_2006Brown:iGEM portal 20062006-07-07T14:27:46Z<p>Agao: </p>
<hr />
<div>{{Brown navigation bar}}<br />
<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0; width: 750px; color: #000000; background-color: #ffffff;"<br />
| width=717px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<h3>Welcome</h3><br />
Welcome to the Brown [http://icampus.mit.edu/projects/iGem.shtml International Genetically Engineered Machine (iGEM)] Team Wiki. Our goal is to participate in the iGEM Competition for the summer of 2006. Feel free to explore our website or [[Brown:Contact | contact us]], we are constantly updating it with the latest developments in the project. <br />
<br />
<h3>Team news </h3><br />
6/18/06 - Funded students - remember you need to add [[User:Johncumbers| a weekly report]] to your user page <br><br />
6/18/06 - Updated [[Brown:Calendar pages |calendar page]], now all info on single page <br><br />
4/22/06 - Please pick up some tasks from the new [[Brown:Todo list | To do list]] <br><br />
3/29/06 - Updated [[Brown:Roles/future planning| Future planning]] section <br><br />
3/29/06 - Please make sure you have signed yourself up for the [[Brown:Journal club:Synthetic biology journal club |journal club]] <br><br />
3/29/06 - If you are in charge of one of the roles listed below, please update your section with your ideas and plans<br />
<br />
|}<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>Team roles</h3><br />
<br />
Team Leaders: [[user:jamielemon|Jamie L.]], [[user:anniegao|Annie]]<br><br />
Fundraising: <br><br />
Secretary: <br> [[user:Victorialattanzi|Victoria]]<br />
Journal Club Coordinator: [[user:petergoldstein|Peter]] <br><br />
Faculty Liason: [[user:azeemkaka| Azeem]]<br><br />
Industry Liason: <br> <br />
Outreach: <br> <br />
[[Brown:Roles/future planning| Future planning]]: [[user:johncumbers|John]] <br> <br />
Chemical DB Manager: [[user:petergoldstein|Peter]] <br><br />
Guest Speaker Organizer: [[user:johncumbers|John]] <br><br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>[[Brown:Todo list |Todo list]]</h3><br />
<h3>[[Brown:Lab Protocols| Lab Protocols]] </h3><br />
<h3>[[Brown:Next Meeting Agenda |Next Meeting Agenda ]]</h3><br />
<br />
<h3>[[Brown:Equipment needed|Equipment needed]]</h3><br />
<br />
<h3>[[Brown:Summer workshops|Summer Workshops]]</h3><br />
<br />
<h3>[[Brown:Calendar pages|Calendar Pages]]</h3><br />
<br />
<h3>[[Brown:Projects |Projects]]</h3><br />
<br />
<h3>Research</h3><br />
#[[Brown:Journal_club:Synthetic_biology_journal_club|Journal Club]]<br />
#[http://www.cumbers.com/igem Idea factory] <br><br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>About Us</h3><br />
#[[Brown:Lab Members | Faculty Advisors]]<br />
#[[Brown:Lab Members | Team Members]]<br />
#[[Brown:Industry Affiliates | Industry Affiliates]]<br />
#[[Brown:March142006 | Meeting minutes]]<br />
<br />
Our lab is in J Walter Wilson, Rm 228, call 401 523 8190<br />
<br />
__NOTOC__</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Calendar_pagesBrown:Calendar pages2006-07-07T14:20:03Z<p>Agao: /* "Summer calendar" */</p>
<hr />
<div>{{Brown navigation bar}}<br />
==Week Five==<br />
Mon 07/03/06: <br><br />
Tue 07/04/06: <br><br />
Wed 07/05/06: Journal club 1.30 PM Walter hall <br><br />
Thu 07/06/06: <br><br />
Fri 07/07/06: 1PM - Weekly Update, JWW 260<br />
<br />
==Week Six==<br />
Mon 07/10/06: <br><br />
Tue 07/11/06: Journal Club, Time TBA<br> <br />
Wed 07/12/06: Samantha Sutton, 12PM Walter Hall<br> <br />
Thu 07/13/06: <br><br />
Fri 07/14/06: 1PM Weekly Meeting, JWW 260 <br><br />
<br />
=="Summer calendar"==<br />
{| style="height:1000px" border="1"<br />
!valign="top" width="100" height="10"| Week <br />
! valign="top" width="100"| Sun <br />
! valign="top" width="100"| Mon <br />
! valign="top" width="100"| Tue <br />
! valign="top" width="100"| Wed <br />
! valign="top" width="100"| Thu <br />
! valign="top" width="100"| Fri <br />
! valign="top" width="100"| Sat<br />
<br />
|-<br />
!1<br />
|4<br />
|5<br />
|6<br />
|7<br> 1PM: Meeting with faculty in JWW conference room<br />
|8<br> 1PM: Bacteria tutorial led by Jamie Gagnon<br />
|9<br> 10AM: APE tutorial led by Azeem and Jason<br />
|10<br />
|-<br />
!2<br />
|11<br />
|12 <br />
|13<br />
|14<br />
|15<br />
|16<br />
|17<br />
|-<br />
!3<br />
|18<br />
|19 <br> 11-1 meeting with Pfiezer<br />
|20<br />
|21 <br> 10AM Meeting with Jay Tang (Bacterial Super Glue)<br />
|22 <br> 10AM-12PM Modeling Tutorial<br />
|23 <br> 10AM BioHazard Training?<br />
1PM Weekly Update, JWW 260<br />
|24<br />
|-<br />
!4<br />
|25<br />
|26<br />
|27<br />
|28 Mac Cowell speak, 2(?)pm WH<br />
|29<br />
|30<br />
|1 July<br />
|-<br />
!5<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
<br />
!6<br />
|9<br />
|10<br />
|11<br />
|12 Samantha Sutton to speak from MIT, 12 noon<br />
|13<br />
|14<br />
|15<br />
|-<br />
<br />
!7<br />
|16<br />
|17<br />
|18<br />
|19<br />
|20<br />
|21<br />
|22<br />
|-<br />
<br />
!8<br />
|23<br />
|24<br />
|25<br />
|26<br />
|27<br />
|28 Jamie L. gone<br />
|29<br />
|-<br />
<br />
!9<br />
|30<br />
|31 Jamie L. back!!<br />
|1 Aug <br />
|2<br />
|3 Annie gone for vacation<br />
|4<br />
|5<br />
|-<br />
<br />
!10<br />
|6<br />
|7<br />
|8 Annie back from vacation<br />
|9<br />
|10<br />
|11<br />
|12<br />
|-<br />
<br />
!11<br />
|13 Jamie L. gone home<br />
|14 Annie gone for the day<br />
|15<br />
|16<br />
|17<br />
|18<br />
|19<br />
|-<br />
<br />
!12<br />
|20<br />
|21<br />
|22<br />
|23<br />
|24<br />
|25<br />
|26<br />
|}<br />
<br />
<h2>Personal calendars</h2><br />
- [[calendar:johncumbers|John Cumbers]]<br><br />
- [[calendar:petergoldstein|Peter Goldstein]]<br><br />
- [[calendar:bhickey|Brendan Hickey]]<br><br />
- [[calendar:anniegao|Annie Gao]] <br><br />
- [[calendar:meganschmidt|Megan Schmidt]]<br><br />
<br />
- Add your name here and copy my page for a template.<br />
<br />
<br />
<br />
==Calendar Archive==<br />
'''Week Three''' <Br><br />
Mon 06/19/06: Pfizer meeting 11-1 at Walter Hall <br><br />
Tue 06/20/06: Journal club 6pm Walter hall <br><br />
Wed 06/21/06: 10am Jay Tang to speak on Bacterial SuperGlue <br><br />
Thu 06/22/06: 10AM-12PM Modeling Tutorial, Walter Hall <br><br />
Fri 06/23/06: health and safety 10.15am, 4th floor conference room Brown office building 1PM - Weekly Update, JWW 260<br />
<br />
'''Week Two''' <Br><br />
Mon 06/12/06: <br />
*john back from vacation, 9.30pm<br />
Tue 06/13/06 <br><br />
*James Brown to visit, arrive ~lunchtime, talk 3-5pm, dinner for all at John's 7pm<br />
Wed 06/14/06 <br><br />
*James Brown to give second talk , 10-12 before leaving for Princeton<br />
<br />
'''Week One''' <br><br />
Mon 06/05/06: <br />
* Morning meeting, plan for this week<br />
* Lab safety training <br><br />
<br><br />
Tue 06/06/06 <br><br />
*11am - 12pm Group meeting. Location: JWW rm. 228 <br> <br><br />
Wed<br><br />
*Mid-week progress talk with faculty- 1pm - 2pm JWW rm. 260<br />
<br />
Thu <br><br />
Fri <br><br />
* afternoon meeting, wrap up the week, plan for next week</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Calendar_pagesBrown:Calendar pages2006-07-07T14:19:23Z<p>Agao: /* "Summer calendar" */</p>
<hr />
<div>{{Brown navigation bar}}<br />
==Week Five==<br />
Mon 07/03/06: <br><br />
Tue 07/04/06: <br><br />
Wed 07/05/06: Journal club 1.30 PM Walter hall <br><br />
Thu 07/06/06: <br><br />
Fri 07/07/06: 1PM - Weekly Update, JWW 260<br />
<br />
==Week Six==<br />
Mon 07/10/06: <br><br />
Tue 07/11/06: Journal Club, Time TBA<br> <br />
Wed 07/12/06: Samantha Sutton, 12PM Walter Hall<br> <br />
Thu 07/13/06: <br><br />
Fri 07/14/06: 1PM Weekly Meeting, JWW 260 <br><br />
<br />
=="Summer calendar"==<br />
{| style="height:1000px" border="1"<br />
!valign="top" width="100" height="10"| Week <br />
! valign="top" width="100"| Sun <br />
! valign="top" width="100"| Mon <br />
! valign="top" width="100"| Tue <br />
! valign="top" width="100"| Wed <br />
! valign="top" width="100"| Thu <br />
! valign="top" width="100"| Fri <br />
! valign="top" width="100"| Sat<br />
<br />
|-<br />
!1<br />
|4<br />
|5<br />
|6<br />
|7<br> 1PM: Meeting with faculty in JWW conference room<br />
|8<br> 1PM: Bacteria tutorial led by Jamie Gagnon<br />
|9<br> 10AM: APE tutorial led by Azeem and Jason<br />
|10<br />
|-<br />
!2<br />
|11<br />
|12 <br />
|13<br />
|14<br />
|15<br />
|16<br />
|17<br />
|-<br />
!3<br />
|18<br />
|19 <br> 11-1 meeting with Pfiezer<br />
|20<br />
|21 <br> 10AM Meeting with Jay Tang (Bacterial Super Glue)<br />
|22 <br> 10AM-12PM Modeling Tutorial<br />
|23 <br> 10AM BioHazard Training?<br />
1PM Weekly Update, JWW 260<br />
|24<br />
|-<br />
!4<br />
|25<br />
|26<br />
|27<br />
|28 Mac Cowell speak, 2(?)pm WH<br />
|29<br />
|30<br />
|1 July<br />
|-<br />
!5<br />
|2<br />
|3<br />
|4<br />
|5<br />
|6<br />
|7<br />
|8<br />
|-<br />
<br />
!6<br />
|9<br />
|10<br />
|11<br />
|12 Samantha Sutton to speak from MIT, 12 noon<br />
|13<br />
|14<br />
|15<br />
|-<br />
<br />
!7<br />
|16<br />
|17<br />
|18<br />
|19<br />
|20<br />
|21<br />
|22<br />
|-<br />
<br />
!8<br />
|23<br />
|24<br />
|25<br />
|26<br />
|27<br />
|28 Jamie L. gone<br />
|29<br />
|-<br />
<br />
!9<br />
|30<br />
|31 Jamie L. back!!<br />
|1 Aug <br />
|2<br />
|3 Annie gone for vacation<br />
|4<br />
|5<br />
|-<br />
<br />
!10<br />
|6<br />
|7<br />
|8 Annie back from vacation<br />
|9<br />
|10<br />
|11<br />
|12Annie gone for the day<br />
|-<br />
<br />
!11<br />
|13 Jamie L. gone home<br />
|14<br />
|15<br />
|16<br />
|17<br />
|18<br />
|19<br />
|-<br />
<br />
!12<br />
|20<br />
|21<br />
|22<br />
|23<br />
|24<br />
|25<br />
|26<br />
|}<br />
<br />
<h2>Personal calendars</h2><br />
- [[calendar:johncumbers|John Cumbers]]<br><br />
- [[calendar:petergoldstein|Peter Goldstein]]<br><br />
- [[calendar:bhickey|Brendan Hickey]]<br><br />
- [[calendar:anniegao|Annie Gao]] <br><br />
- [[calendar:meganschmidt|Megan Schmidt]]<br><br />
<br />
- Add your name here and copy my page for a template.<br />
<br />
<br />
<br />
==Calendar Archive==<br />
'''Week Three''' <Br><br />
Mon 06/19/06: Pfizer meeting 11-1 at Walter Hall <br><br />
Tue 06/20/06: Journal club 6pm Walter hall <br><br />
Wed 06/21/06: 10am Jay Tang to speak on Bacterial SuperGlue <br><br />
Thu 06/22/06: 10AM-12PM Modeling Tutorial, Walter Hall <br><br />
Fri 06/23/06: health and safety 10.15am, 4th floor conference room Brown office building 1PM - Weekly Update, JWW 260<br />
<br />
'''Week Two''' <Br><br />
Mon 06/12/06: <br />
*john back from vacation, 9.30pm<br />
Tue 06/13/06 <br><br />
*James Brown to visit, arrive ~lunchtime, talk 3-5pm, dinner for all at John's 7pm<br />
Wed 06/14/06 <br><br />
*James Brown to give second talk , 10-12 before leaving for Princeton<br />
<br />
'''Week One''' <br><br />
Mon 06/05/06: <br />
* Morning meeting, plan for this week<br />
* Lab safety training <br><br />
<br><br />
Tue 06/06/06 <br><br />
*11am - 12pm Group meeting. Location: JWW rm. 228 <br> <br><br />
Wed<br><br />
*Mid-week progress talk with faculty- 1pm - 2pm JWW rm. 260<br />
<br />
Thu <br><br />
Fri <br><br />
* afternoon meeting, wrap up the week, plan for next week</div>Agaohttp://2006.igem.org/wiki/index.php/User:AnniegaoUser:Anniegao2006-07-07T14:18:01Z<p>Agao: </p>
<hr />
<div>Year: 2009 <br><br />
Concentration: Biology<br><br />
Hometown: East Lyme, CT<br />
<br />
<br />
== Weekly Reports==<br />
<Br><br />
<br />
'''Week Four'''<Br>I helped to divide our new idea of the bacterial freeze tag into four smaller projects. Jamie Lemon and I began working on the project called the freeze machine. We successfully transformed all of the parts required for this project. We also began miniprepping our parts, but were unable to finish due to a shortage of miniprep columns. We will finish the minipreps next week and then proceed onto digestion and ligations.<br />
<br><br />
'''Week Five'''<br><br />
Wednesday: Performed transformations for Freeze machine parts. Made Amp. plates.<br />
Thursday: Updated wiki with Jamie Lemon and made of list of tasks for the group. We also grew up colonies for a miniprep tomorrow.</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:March142006Brown:March1420062006-07-07T14:13:54Z<p>Agao: /* Meeting Minutes for June 30, 2006 */</p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br />
==Meeting Minutes for June 30, 2006==<br />
Agenda:<br><br />
1. Update on project: split into three projects<br><br />
2. Magnetotactic bacteria<br><br />
3. everyone happy?<br><br />
4. Actions <br><br />
<br />
'''Meeting Notes:'''<br><br />
1. Update on project:<br><br />
- Freeze machine: transformed all parts<br><br />
- Sender: parts grown up, will start minipreps, restriction digests<br><br />
- Unfreeze machine: parts grew<br><br />
- Receiver: grew up parts for miniprep<br><br />
2. Magnetotactic Bacteria<br><br />
- they are growing<br><br />
- wait five to seven days.<br><br />
- plasmid: Peter: hasn’t found information. Needs help<br><br />
- Hayato sent Tayhas an email and she has sent it regarding plasmid.<br> <br />
3. Money update<br><br />
- we have 8000 in account<br><br />
- Brenden will go and ask Carol<br> <br />
4. Device<br><br />
- Azeem will talk to Professor Tripathi about building microfluidics chamber<br><br />
- Guest speaker?<br><br />
5. Modeling<br><br />
- installing software<br><br />
- Peter’s been using it. Has produced model of Azeem’s paper<br><br />
6. Guest speakers<br><br />
- reason is to have communications with other people in synthetic biology<br><br />
- Somebody organizes the guest speaker and that organizer looks after the guest speaker. This job is shared around the department. Would this work for iGEM?<br><br />
- People talk about project with guest speaker. Sign up for 45 minutes or so.<br><br />
- Send out an email about signing up to talking with guest lecturer<br><br />
7. Weekly meetings<br><br />
- email people when you can’t come<br><br />
8. Sequencing:<br><br />
- To avoid sequencing, restriction digest and check with around right size with DNA ladder. <br><br />
- Limit: 800 base pairs<br><br />
- If longer than 800 base pairs, can order an oligo.<br><br />
- Gene Wiz- someone on the third floor<br><br />
- Prep the samples and send out. See Jamie for help. <br><br />
9. Pfizer<br><br />
- Send a thank you note and the wish list- Victoria<br><br />
10. Project<br><br />
- Brenden will draw pictures in photoshop<br><br />
- Have a few to explain each part. <br><br />
- Somebody put the idea onto the wiki<br><br />
- Put everything on the public wiki<br><br />
- Peter will move everything from private wiki to the public wiki<br><br />
- Brenden to organize group picture<br><br />
<br />
==Meeting Minutes for March 14, 2006==<br />
<br />
===Assignment of roles===<br />
<br />
'''Community Maker''' - Nick, Megan<br />
<br />
'''Notetaker/Organizer (Secretary)''' - Angela<br />
<br />
'''Journal Club Coordinator''' - Elena<br />
<br />
'''Fundraising/PR'''<br />
<br />
* Julie: Fundraising, Sheldon<br />
<br />
* Brendan: PR<br />
<br />
* Elaine: helping both<br />
<br />
'''Faculty Liaison''' - Victoria<br />
<br />
'''Wiki Development''' - Peter<br />
<br />
'''Research''' - Annie, Sheldon<br />
<br />
'''"Benevolent Dictator"''' =) - John<br />
<br />
'''Commercialization Potential''' (patents, etc)<br />
<br />
'''Industry Laison'''<br />
<br />
We have lab space in JWW!!!<br />
<br />
==Meeting Minutes for March 21, 2006==<br />
<br />
Present: John, Kara, Angela, Annie, Jessie, Peter, Brendan, Katherine, Victoria, Megan<br />
<br />
===Journal Club Presentation===<br />
<br />
====Construction of a Genetic Toggle Switch in E. Coli====<br />
<br />
by Victoria and Megan<br />
<br />
*purpose: to integrate theory and experiment by constructing and testing a synthetic, bistable gene circuit (Toggle switch) based on the predictions of a simple mathematical model<br />
*integrate (mathetic) theory and (biology) experiment<br />
*mathematical models supposed to predict how much transcribed, testing expected outcomes<br />
*took a switch that self-regulates and has inducer (heat, chemical) that starts<br />
*prediction was wrong due to gene variability<br />
*simplest, as few reagents as possible better<br />
*specialized promoters <br />
*ribozyme binding sites, harnessed in plasmid; ensure that they are turned into proteins individually<br />
*genetic engineering (memory device) insert into something that you would want to be turned on for a long time<br />
*registry of biological parts at MIT; biological switch, wire linked together (electronic diagramà biological diagram<br />
<br />
===2005 iGEM project summaries:===<br />
<br />
UCSF: Kara; genetic circuit of bacteria to respond to temperature gradient, analog vs. digital<br />
<br />
Toronto: Angela; Cell See-Us Thermometer and Bacterial Etch-a-Sketch; used mRFP and GFP didn’t have a result<br />
<br />
Berkeley: John; cell-cell communicator, send out genomes to other bacteria via a specific pore (channel) and the other bacteria would send something back when it received that message<br />
<br />
Harvard: Peter; bio-sketch; use UV pen to write on bacteria, use GFP mutation as reporter; not so much a toggle switch as a one way switch; used heat to “erase”; didn’t work<br />
<br />
Caltech: Annie; detect caffeine in solution; YFP and GFP high concentration of caffeine would repress GFP and YFP would glow; if decaf, YFP would be repressed and GFP would grow; if medium, then both would be present.<br />
<br />
==Meeting Minutes for April 4, 2006==<br />
<br />
Present: Jamie, Azime, John, Angela, Bo, Brendan, Peter, Jason, Jamie, Victoria Elena<br />
<br />
===Journal Club Presentation===<br />
<br />
====Engineering a pathway in E. Coli for turpenoids====<br />
<br />
by Brendan and Peter <br />
<br />
*turponoids - organic compound for making steroids<br />
*antimalarial medicine, etc. (anticancer, antihallucenogenic)<br />
*purified from plants, extracted from plants and then synthesized – limits<br />
*pathway from yeast and put into E. Coli <br />
*PCR created 3 mutant pathways, point mutations which inserted into E. Coli<br />
*2 of the sequences worked; combined the two (beginning of one and end of another)<br />
*isoprene 5C structure; double, single, double building blocks<br />
*IPP is inhibitor that halts cell growth (for MAP)<br />
*amorphodyein synthase consumes IPP, increases levels of mevalonate acid without halting cell growth<br />
*pathway doesn’t exist in nature; this pathway 10-300 fold increase in production<br />
*head of synthetic bio department of Berkeley (Gates Foundation gave lots of funding)<br />
*Melavonatic → MevP → MevPP → IPP &→ DMAPP<br />
::genes: -ERG12 -ERG8 -MVD -1d1 = these genes are in plants but not in E. Coli, so codon optimization corrects for differences in codons between bacteria and plants<br />
*then stepped back to beginning: melavonatic not a good precursor, so XYZ converts acetyl CoA (naturally occurring in cell and a lot *cheaper) into Melavonatic Acid (which is hard to get)<br />
concerned about product yield and amounts of cells, so another step was making sure that [IPP] kept low à keeping cells alive as we put in genes, etc.<br />
<br />
===Notes:===<br />
<br />
*forward papers the week before to John so that he can make copies of diagrams, etc.<br />
*there will be workshops at beginning of summer about PCR, etc.<br />
*Ideas: get some more detail (make a concise description of) and let the faculty know about them and get their opinions on whether they are viable, linked to current faculty research:<br />
*#John: cell division counter – when (yeast) cells aging, different colors emitted from fluorochrome (like binary signal, red=1, blue =2, green = 3, etc. counts up to 32) if besides bacteria, takes longer to take up; bacteria, e.coli overnight; Harvard last year tried doing this; first round fine, but second on, harder; link color to cdk when transcribed, produced fluorochrome, activator and binds to promoter region of next and then repressor on next represses color from first one while another activator produces another color<br />
*#Julie: bacteria producing alkanes – products poisoning cells (alcohols, alkanes that act as detergents on membrane); talk to Dept of Energy? (speaker) – Prof Moulton<br />
*#analog devices: transistors scalable super computing; the more the cells grow, the more computational power you have; producing something useful is impractical; quorum sensing? like Minesweeper - Jamie<br />
*#bio sensors and actuators – live inside body and transmit info out; bio sensors sense the signals and actuators would amplify getting info of cell out (more complex than on/off); possibly like tumors<br />
*#free radical reporter - Victoria<br />
*#chemical detector – assembly line from input to receiver (like Berkeley team) Annie<br />
*#artificial organelle<br />
*#microbes that maintain/fix environment<br />
*#bacterial eye/radar – emit light (through reporter) and if reflects back, send out signal<br />
<br />
==ACTIONS==<br />
<br />
===March 14, 2006===<br />
* Victoria will go to SAO to get info about table for ADOCH<br />
* possibly also a web page/link to Brown iGEM<br />
* have a site for prospective students to see who we are, as well as our contact info<br />
* also, for everyone: think of ways to gain more attention <br />
<br />
* Everyone: create a section on the wiki for the category you are overseeing<br />
<br />
* Create a community portal:<br />
** serve as a site that can be linked possibly from admissions page<br />
** emphasizing that we are cross-disciplinary undergraduates performing research at Brown University, even several freshmen involved<br />
** have our slightly more colorful biolographical infos attached<br />
<br />
* Possible ideas for our project?<br />
<br />
* Victoria/Megan doing the Journal Club presentation next week.<br />
<br />
===March 21, 2006===<br />
<br />
Come up with an idea by first meeting after Spring Break<br />
<br />
Journal Club Presenters: (in order of presentation): talk to faculty about them (we have 19!!!) send out papers to faculty early just to make sure.<br />
<br />
*Peter and Brendan 4/4<br />
<br />
*Angela and Annie 4/11<br />
<br />
*Kara and Jessie 4/18<br />
<br />
*Katherine and John 4/25<br />
<br />
===April 4, 2006===<br />
Journal projects for future:<br />
*Angela and Annie 4/11<br />
*Kara and Jessie 4/18<br />
*Katherine and John 4/25<br />
*Jason and Azime 5/2<br />
*Elena and Jamie 5/9<br />
<br />
Remember to decide a week ahead of time so that faculty can be contacted and help with your paper.<br />
Also remember that you can email John diagrams, etc. so that he can make copies for everyone at the Journal Club meeting.<br />
<br />
<br />
Make concise descriptions of our ideas to send to faculty</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:March142006Brown:March1420062006-07-07T14:11:04Z<p>Agao: /* Meeting Minutes for June 30, 2006 */</p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br />
==Meeting Minutes for June 30, 2006==<br />
Agenda:<br><br />
1. Update on project: split into three projects<br><br />
2. Magnetotactic bacteria<br><br />
3. everyone happy?<br><br />
4. Actions <br><br />
<br />
'''Meeting Notes:'''<br><br />
1. Update on project:<br><br />
- Freeze machine: transformed all parts<br><br />
- Sender: parts grown up, will start minipreps, restriction digests<br><br />
- Unfreeze machine: parts grew<br><br />
- Receiver: grew up parts for miniprep<br><br />
2. Magnetotactic Bacteria<br><br />
- they are growing<br><br />
- wait five to seven days.<br><br />
- plasmid: Peter: hasn’t found information. Needs help<br><br />
- Hayato sent Tayhas an email and she has sent it regarding plasmid.<br> <br />
3. Money update<br><br />
- we have 8000 in account<br><br />
- Brenden will go and ask Carol<br> <br />
4. Device<br><br />
- Azeem will talk to Professor Tripathi about building microfluidics chamber<br />
- Guest speaker?<br />
5. Modeling<br />
- installing software<br />
- Peter’s been using it. Has produced model of Azeem’s paper<br />
6. Guest speakers<br />
- reason is to have communications with other people in synthetic biology<br />
- Somebody organizes the guest speaker and that organizer looks after the guest speaker. This job is shared around the department. Would this work for iGEM?<br />
- People talk about project with guest speaker. Sign up for 45 minutes or so.<br />
- Send out an email about signing up to talking with guest lecturer<br />
7. Weekly meetings<br />
- email people when you can’t come<br />
8. Sequencing:<br />
- To avoid sequencing, restriction digest and check with around right size with DNA ladder. <br />
- Limit: 100 base pairs<br />
- If longer than 800 base pairs, can order an oligo.<br />
- Gene Wiz- someone on the third floor<br />
- Prep the samples and send out. See Jamie for help. <br />
9. Pfizer<br />
- Send a thank you note and the wish list- Victoria<br />
10. Project<br />
- Brenden will draw pictures in photoshop<br />
- Have a few to explain each part. <br />
- Somebody put the idea onto the wiki<br />
- Put everything on the public wiki<br />
- Peter will move everything from private wiki to the public wiki<br />
- Brenden to organize group picture<br />
<br />
==Meeting Minutes for March 14, 2006==<br />
<br />
===Assignment of roles===<br />
<br />
'''Community Maker''' - Nick, Megan<br />
<br />
'''Notetaker/Organizer (Secretary)''' - Angela<br />
<br />
'''Journal Club Coordinator''' - Elena<br />
<br />
'''Fundraising/PR'''<br />
<br />
* Julie: Fundraising, Sheldon<br />
<br />
* Brendan: PR<br />
<br />
* Elaine: helping both<br />
<br />
'''Faculty Liaison''' - Victoria<br />
<br />
'''Wiki Development''' - Peter<br />
<br />
'''Research''' - Annie, Sheldon<br />
<br />
'''"Benevolent Dictator"''' =) - John<br />
<br />
'''Commercialization Potential''' (patents, etc)<br />
<br />
'''Industry Laison'''<br />
<br />
We have lab space in JWW!!!<br />
<br />
==Meeting Minutes for March 21, 2006==<br />
<br />
Present: John, Kara, Angela, Annie, Jessie, Peter, Brendan, Katherine, Victoria, Megan<br />
<br />
===Journal Club Presentation===<br />
<br />
====Construction of a Genetic Toggle Switch in E. Coli====<br />
<br />
by Victoria and Megan<br />
<br />
*purpose: to integrate theory and experiment by constructing and testing a synthetic, bistable gene circuit (Toggle switch) based on the predictions of a simple mathematical model<br />
*integrate (mathetic) theory and (biology) experiment<br />
*mathematical models supposed to predict how much transcribed, testing expected outcomes<br />
*took a switch that self-regulates and has inducer (heat, chemical) that starts<br />
*prediction was wrong due to gene variability<br />
*simplest, as few reagents as possible better<br />
*specialized promoters <br />
*ribozyme binding sites, harnessed in plasmid; ensure that they are turned into proteins individually<br />
*genetic engineering (memory device) insert into something that you would want to be turned on for a long time<br />
*registry of biological parts at MIT; biological switch, wire linked together (electronic diagramà biological diagram<br />
<br />
===2005 iGEM project summaries:===<br />
<br />
UCSF: Kara; genetic circuit of bacteria to respond to temperature gradient, analog vs. digital<br />
<br />
Toronto: Angela; Cell See-Us Thermometer and Bacterial Etch-a-Sketch; used mRFP and GFP didn’t have a result<br />
<br />
Berkeley: John; cell-cell communicator, send out genomes to other bacteria via a specific pore (channel) and the other bacteria would send something back when it received that message<br />
<br />
Harvard: Peter; bio-sketch; use UV pen to write on bacteria, use GFP mutation as reporter; not so much a toggle switch as a one way switch; used heat to “erase”; didn’t work<br />
<br />
Caltech: Annie; detect caffeine in solution; YFP and GFP high concentration of caffeine would repress GFP and YFP would glow; if decaf, YFP would be repressed and GFP would grow; if medium, then both would be present.<br />
<br />
==Meeting Minutes for April 4, 2006==<br />
<br />
Present: Jamie, Azime, John, Angela, Bo, Brendan, Peter, Jason, Jamie, Victoria Elena<br />
<br />
===Journal Club Presentation===<br />
<br />
====Engineering a pathway in E. Coli for turpenoids====<br />
<br />
by Brendan and Peter <br />
<br />
*turponoids - organic compound for making steroids<br />
*antimalarial medicine, etc. (anticancer, antihallucenogenic)<br />
*purified from plants, extracted from plants and then synthesized – limits<br />
*pathway from yeast and put into E. Coli <br />
*PCR created 3 mutant pathways, point mutations which inserted into E. Coli<br />
*2 of the sequences worked; combined the two (beginning of one and end of another)<br />
*isoprene 5C structure; double, single, double building blocks<br />
*IPP is inhibitor that halts cell growth (for MAP)<br />
*amorphodyein synthase consumes IPP, increases levels of mevalonate acid without halting cell growth<br />
*pathway doesn’t exist in nature; this pathway 10-300 fold increase in production<br />
*head of synthetic bio department of Berkeley (Gates Foundation gave lots of funding)<br />
*Melavonatic → MevP → MevPP → IPP &→ DMAPP<br />
::genes: -ERG12 -ERG8 -MVD -1d1 = these genes are in plants but not in E. Coli, so codon optimization corrects for differences in codons between bacteria and plants<br />
*then stepped back to beginning: melavonatic not a good precursor, so XYZ converts acetyl CoA (naturally occurring in cell and a lot *cheaper) into Melavonatic Acid (which is hard to get)<br />
concerned about product yield and amounts of cells, so another step was making sure that [IPP] kept low à keeping cells alive as we put in genes, etc.<br />
<br />
===Notes:===<br />
<br />
*forward papers the week before to John so that he can make copies of diagrams, etc.<br />
*there will be workshops at beginning of summer about PCR, etc.<br />
*Ideas: get some more detail (make a concise description of) and let the faculty know about them and get their opinions on whether they are viable, linked to current faculty research:<br />
*#John: cell division counter – when (yeast) cells aging, different colors emitted from fluorochrome (like binary signal, red=1, blue =2, green = 3, etc. counts up to 32) if besides bacteria, takes longer to take up; bacteria, e.coli overnight; Harvard last year tried doing this; first round fine, but second on, harder; link color to cdk when transcribed, produced fluorochrome, activator and binds to promoter region of next and then repressor on next represses color from first one while another activator produces another color<br />
*#Julie: bacteria producing alkanes – products poisoning cells (alcohols, alkanes that act as detergents on membrane); talk to Dept of Energy? (speaker) – Prof Moulton<br />
*#analog devices: transistors scalable super computing; the more the cells grow, the more computational power you have; producing something useful is impractical; quorum sensing? like Minesweeper - Jamie<br />
*#bio sensors and actuators – live inside body and transmit info out; bio sensors sense the signals and actuators would amplify getting info of cell out (more complex than on/off); possibly like tumors<br />
*#free radical reporter - Victoria<br />
*#chemical detector – assembly line from input to receiver (like Berkeley team) Annie<br />
*#artificial organelle<br />
*#microbes that maintain/fix environment<br />
*#bacterial eye/radar – emit light (through reporter) and if reflects back, send out signal<br />
<br />
==ACTIONS==<br />
<br />
===March 14, 2006===<br />
* Victoria will go to SAO to get info about table for ADOCH<br />
* possibly also a web page/link to Brown iGEM<br />
* have a site for prospective students to see who we are, as well as our contact info<br />
* also, for everyone: think of ways to gain more attention <br />
<br />
* Everyone: create a section on the wiki for the category you are overseeing<br />
<br />
* Create a community portal:<br />
** serve as a site that can be linked possibly from admissions page<br />
** emphasizing that we are cross-disciplinary undergraduates performing research at Brown University, even several freshmen involved<br />
** have our slightly more colorful biolographical infos attached<br />
<br />
* Possible ideas for our project?<br />
<br />
* Victoria/Megan doing the Journal Club presentation next week.<br />
<br />
===March 21, 2006===<br />
<br />
Come up with an idea by first meeting after Spring Break<br />
<br />
Journal Club Presenters: (in order of presentation): talk to faculty about them (we have 19!!!) send out papers to faculty early just to make sure.<br />
<br />
*Peter and Brendan 4/4<br />
<br />
*Angela and Annie 4/11<br />
<br />
*Kara and Jessie 4/18<br />
<br />
*Katherine and John 4/25<br />
<br />
===April 4, 2006===<br />
Journal projects for future:<br />
*Angela and Annie 4/11<br />
*Kara and Jessie 4/18<br />
*Katherine and John 4/25<br />
*Jason and Azime 5/2<br />
*Elena and Jamie 5/9<br />
<br />
Remember to decide a week ahead of time so that faculty can be contacted and help with your paper.<br />
Also remember that you can email John diagrams, etc. so that he can make copies for everyone at the Journal Club meeting.<br />
<br />
<br />
Make concise descriptions of our ideas to send to faculty</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:March142006Brown:March1420062006-07-07T14:09:43Z<p>Agao: /* Meeting Minutes for March 14, 2006 */</p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br />
==Meeting Minutes for June 30, 2006==<br />
Agenda:<br />
1. Update on project: split into three projects<br />
2. Magnetotactic bacteria<br />
3. everyone happy?<br />
4. Actions <br />
<br />
Meeting Notes:<br />
1. Update on project:<br />
- Freeze machine: transformed all parts<br />
- Sender: parts grown up, will start minipreps, restriction digests<br />
- Unfreeze machine: parts grew<br />
- Receiver: grew up parts for miniprep<br />
2. Magnetotactic Bacteria<br />
- they are growing<br />
- wait five to seven days.<br />
- plasmid: Peter: hasn’t found information. Needs help<br />
- Hayato sent Tayhas an email and she has sent it regarding plasmid. <br />
3. Money update<br />
- we have 8000 in account<br />
- Brenden will go and ask Carol <br />
4. Device<br />
- Azeem will talk to Professor Tripathi about building microfluidics chamber<br />
- Guest speaker?<br />
5. Modeling<br />
- installing software<br />
- Peter’s been using it. Has produced model of Azeem’s paper<br />
6. Guest speakers<br />
- reason is to have communications with other people in synthetic biology<br />
- Somebody organizes the guest speaker and that organizer looks after the guest speaker. This job is shared around the department. Would this work for iGEM?<br />
- People talk about project with guest speaker. Sign up for 45 minutes or so.<br />
- Send out an email about signing up to talking with guest lecturer<br />
7. Weekly meetings<br />
- email people when you can’t come<br />
8. Sequencing:<br />
- To avoid sequencing, restriction digest and check with around right size with DNA ladder. <br />
- Limit: 100 base pairs<br />
- If longer than 800 base pairs, can order an oligo.<br />
- Gene Wiz- someone on the third floor<br />
- Prep the samples and send out. See Jamie for help. <br />
9. Pfizer<br />
- Send a thank you note and the wish list- Victoria<br />
10. Project<br />
- Brenden will draw pictures in photoshop<br />
- Have a few to explain each part. <br />
- Somebody put the idea onto the wiki<br />
- Put everything on the public wiki<br />
- Peter will move everything from private wiki to the public wiki<br />
- Brenden to organize group picture <br />
<br />
==Meeting Minutes for March 14, 2006==<br />
<br />
===Assignment of roles===<br />
<br />
'''Community Maker''' - Nick, Megan<br />
<br />
'''Notetaker/Organizer (Secretary)''' - Angela<br />
<br />
'''Journal Club Coordinator''' - Elena<br />
<br />
'''Fundraising/PR'''<br />
<br />
* Julie: Fundraising, Sheldon<br />
<br />
* Brendan: PR<br />
<br />
* Elaine: helping both<br />
<br />
'''Faculty Liaison''' - Victoria<br />
<br />
'''Wiki Development''' - Peter<br />
<br />
'''Research''' - Annie, Sheldon<br />
<br />
'''"Benevolent Dictator"''' =) - John<br />
<br />
'''Commercialization Potential''' (patents, etc)<br />
<br />
'''Industry Laison'''<br />
<br />
We have lab space in JWW!!!<br />
<br />
==Meeting Minutes for March 21, 2006==<br />
<br />
Present: John, Kara, Angela, Annie, Jessie, Peter, Brendan, Katherine, Victoria, Megan<br />
<br />
===Journal Club Presentation===<br />
<br />
====Construction of a Genetic Toggle Switch in E. Coli====<br />
<br />
by Victoria and Megan<br />
<br />
*purpose: to integrate theory and experiment by constructing and testing a synthetic, bistable gene circuit (Toggle switch) based on the predictions of a simple mathematical model<br />
*integrate (mathetic) theory and (biology) experiment<br />
*mathematical models supposed to predict how much transcribed, testing expected outcomes<br />
*took a switch that self-regulates and has inducer (heat, chemical) that starts<br />
*prediction was wrong due to gene variability<br />
*simplest, as few reagents as possible better<br />
*specialized promoters <br />
*ribozyme binding sites, harnessed in plasmid; ensure that they are turned into proteins individually<br />
*genetic engineering (memory device) insert into something that you would want to be turned on for a long time<br />
*registry of biological parts at MIT; biological switch, wire linked together (electronic diagramà biological diagram<br />
<br />
===2005 iGEM project summaries:===<br />
<br />
UCSF: Kara; genetic circuit of bacteria to respond to temperature gradient, analog vs. digital<br />
<br />
Toronto: Angela; Cell See-Us Thermometer and Bacterial Etch-a-Sketch; used mRFP and GFP didn’t have a result<br />
<br />
Berkeley: John; cell-cell communicator, send out genomes to other bacteria via a specific pore (channel) and the other bacteria would send something back when it received that message<br />
<br />
Harvard: Peter; bio-sketch; use UV pen to write on bacteria, use GFP mutation as reporter; not so much a toggle switch as a one way switch; used heat to “erase”; didn’t work<br />
<br />
Caltech: Annie; detect caffeine in solution; YFP and GFP high concentration of caffeine would repress GFP and YFP would glow; if decaf, YFP would be repressed and GFP would grow; if medium, then both would be present.<br />
<br />
==Meeting Minutes for April 4, 2006==<br />
<br />
Present: Jamie, Azime, John, Angela, Bo, Brendan, Peter, Jason, Jamie, Victoria Elena<br />
<br />
===Journal Club Presentation===<br />
<br />
====Engineering a pathway in E. Coli for turpenoids====<br />
<br />
by Brendan and Peter <br />
<br />
*turponoids - organic compound for making steroids<br />
*antimalarial medicine, etc. (anticancer, antihallucenogenic)<br />
*purified from plants, extracted from plants and then synthesized – limits<br />
*pathway from yeast and put into E. Coli <br />
*PCR created 3 mutant pathways, point mutations which inserted into E. Coli<br />
*2 of the sequences worked; combined the two (beginning of one and end of another)<br />
*isoprene 5C structure; double, single, double building blocks<br />
*IPP is inhibitor that halts cell growth (for MAP)<br />
*amorphodyein synthase consumes IPP, increases levels of mevalonate acid without halting cell growth<br />
*pathway doesn’t exist in nature; this pathway 10-300 fold increase in production<br />
*head of synthetic bio department of Berkeley (Gates Foundation gave lots of funding)<br />
*Melavonatic → MevP → MevPP → IPP &→ DMAPP<br />
::genes: -ERG12 -ERG8 -MVD -1d1 = these genes are in plants but not in E. Coli, so codon optimization corrects for differences in codons between bacteria and plants<br />
*then stepped back to beginning: melavonatic not a good precursor, so XYZ converts acetyl CoA (naturally occurring in cell and a lot *cheaper) into Melavonatic Acid (which is hard to get)<br />
concerned about product yield and amounts of cells, so another step was making sure that [IPP] kept low à keeping cells alive as we put in genes, etc.<br />
<br />
===Notes:===<br />
<br />
*forward papers the week before to John so that he can make copies of diagrams, etc.<br />
*there will be workshops at beginning of summer about PCR, etc.<br />
*Ideas: get some more detail (make a concise description of) and let the faculty know about them and get their opinions on whether they are viable, linked to current faculty research:<br />
*#John: cell division counter – when (yeast) cells aging, different colors emitted from fluorochrome (like binary signal, red=1, blue =2, green = 3, etc. counts up to 32) if besides bacteria, takes longer to take up; bacteria, e.coli overnight; Harvard last year tried doing this; first round fine, but second on, harder; link color to cdk when transcribed, produced fluorochrome, activator and binds to promoter region of next and then repressor on next represses color from first one while another activator produces another color<br />
*#Julie: bacteria producing alkanes – products poisoning cells (alcohols, alkanes that act as detergents on membrane); talk to Dept of Energy? (speaker) – Prof Moulton<br />
*#analog devices: transistors scalable super computing; the more the cells grow, the more computational power you have; producing something useful is impractical; quorum sensing? like Minesweeper - Jamie<br />
*#bio sensors and actuators – live inside body and transmit info out; bio sensors sense the signals and actuators would amplify getting info of cell out (more complex than on/off); possibly like tumors<br />
*#free radical reporter - Victoria<br />
*#chemical detector – assembly line from input to receiver (like Berkeley team) Annie<br />
*#artificial organelle<br />
*#microbes that maintain/fix environment<br />
*#bacterial eye/radar – emit light (through reporter) and if reflects back, send out signal<br />
<br />
==ACTIONS==<br />
<br />
===March 14, 2006===<br />
* Victoria will go to SAO to get info about table for ADOCH<br />
* possibly also a web page/link to Brown iGEM<br />
* have a site for prospective students to see who we are, as well as our contact info<br />
* also, for everyone: think of ways to gain more attention <br />
<br />
* Everyone: create a section on the wiki for the category you are overseeing<br />
<br />
* Create a community portal:<br />
** serve as a site that can be linked possibly from admissions page<br />
** emphasizing that we are cross-disciplinary undergraduates performing research at Brown University, even several freshmen involved<br />
** have our slightly more colorful biolographical infos attached<br />
<br />
* Possible ideas for our project?<br />
<br />
* Victoria/Megan doing the Journal Club presentation next week.<br />
<br />
===March 21, 2006===<br />
<br />
Come up with an idea by first meeting after Spring Break<br />
<br />
Journal Club Presenters: (in order of presentation): talk to faculty about them (we have 19!!!) send out papers to faculty early just to make sure.<br />
<br />
*Peter and Brendan 4/4<br />
<br />
*Angela and Annie 4/11<br />
<br />
*Kara and Jessie 4/18<br />
<br />
*Katherine and John 4/25<br />
<br />
===April 4, 2006===<br />
Journal projects for future:<br />
*Angela and Annie 4/11<br />
*Kara and Jessie 4/18<br />
*Katherine and John 4/25<br />
*Jason and Azime 5/2<br />
*Elena and Jamie 5/9<br />
<br />
Remember to decide a week ahead of time so that faculty can be contacted and help with your paper.<br />
Also remember that you can email John diagrams, etc. so that he can make copies for everyone at the Journal Club meeting.<br />
<br />
<br />
Make concise descriptions of our ideas to send to faculty</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:iGEM_portal_2006Brown:iGEM portal 20062006-07-07T14:06:13Z<p>Agao: </p>
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<h3>Welcome</h3><br />
Welcome to the Brown [http://icampus.mit.edu/projects/iGem.shtml International Genetically Engineered Machine (iGEM)] Team Wiki. Our goal is to participate in the iGEM Competition for the summer of 2006. Feel free to explore our website or [[Brown:Contact | contact us]], we are constantly updating it with the latest developments in the project. <br />
<br />
<h3>Team news </h3><br />
6/18/06 - Funded students - remember you need to add [[User:Johncumbers| a weekly report]] to your user page <br><br />
6/18/06 - Updated [[Brown:Calendar pages |calendar page]], now all info on single page <br><br />
4/22/06 - Please pick up some tasks from the new [[Brown:Todo list | To do list]] <br><br />
3/29/06 - Updated [[Brown:Roles/future planning| Future planning]] section <br><br />
3/29/06 - Please make sure you have signed yourself up for the [[Brown:Journal club:Synthetic biology journal club |journal club]] <br><br />
3/29/06 - If you are in charge of one of the roles listed below, please update your section with your ideas and plans<br />
<br />
|}<br />
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|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>Team roles</h3><br />
<br />
Team Leaders: [[user:jamielemon|Jamie L.]], [[user:anniegao|Annie]]<br><br />
Fundraising: <br><br />
Secretary: <br><br />
Journal Club Coordinator: [[user:petergoldstein|Peter]] <br><br />
Faculty Liason: [[user:azeemkaka| Azeem]]<br><br />
Industry Liason: <br> <br />
Outreach: <br> <br />
[[Brown:Roles/future planning| Future planning]]: [[user:johncumbers|John]] <br> <br />
Chemical DB Manager: [[user:petergoldstein|Peter]] <br><br />
Guest Speaker Organizer: [[user:johncumbers|John]] <br><br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>[[Brown:Todo list |Todo list]]</h3><br />
<h3>[[Brown:Lab Protocols| Lab Protocols]] </h3><br />
<h3>[[Brown:Next Meeting Agenda |Next Meeting Agenda ]]</h3><br />
<br />
<h3>[[Brown:Equipment needed|Equipment needed]]</h3><br />
<br />
<h3>[[Brown:Summer workshops|Summer Workshops]]</h3><br />
<br />
<h3>[[Brown:Calendar pages|Calendar Pages]]</h3><br />
<br />
<h3>[[Brown:Projects |Projects]]</h3><br />
<br />
<h3>Research</h3><br />
#[[Brown:Journal_club:Synthetic_biology_journal_club|Journal Club]]<br />
#[http://www.cumbers.com/igem Idea factory] <br><br />
<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>About Us</h3><br />
#[[Brown:Lab Members | Faculty Advisors]]<br />
#[[Brown:Lab Members | Team Members]]<br />
#[[Brown:Industry Affiliates | Industry Affiliates]]<br />
#[[Brown:March142006 | Meeting minutes]]<br />
<br />
Our lab is in J Walter Wilson, Rm 228, call 401 523 8190<br />
<br />
__NOTOC__</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Lab_ProtocolsBrown:Lab Protocols2006-07-07T14:05:58Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br> <br><br />
[[Brown:Transformation Protocol with our competent cells | '''Transformation Protocol with our competent cells''']]<br> <br><br />
[[Brown:Transformation Protocol with Fischer cells | '''Transformation Protocol with Fischer cells''']]<br> <br><br />
[[Brown:Gel Protocol | '''Gel Protocol''']]<br><br><br />
[[Brown:TAE solution Protocols| '''TAE solution Protocols''']]<br><br><br />
[[Brown:Protocols|'''Magnetotactic Bacteria''']]</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Lab_ProtocolsBrown:Lab Protocols2006-07-07T14:05:47Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br> <br><br />
[[Brown:Transformation Protocol with our competent cells | '''Transformation Protocol with our competent cells''']]<br> <br><br />
[[Brown:Transformation Protocol with Fischer cells | '''Transformation Protocol with Fischer cells''']]<br> <br><br />
[[Brown:Gel Protocol | '''Gel Protocol''']]<br><br><br />
[[Brown:TAE solution Protocols| '''TAE solution Protocols''']]<br><br><br />
[[Brown:Protocols|Magnetotactic Bacteria]]</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Lab_ProtocolsBrown:Lab Protocols2006-07-07T14:05:28Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br> <br><br />
[[Brown:Transformation Protocol with our competent cells | '''Transformation Protocol with our competent cells''']]<br> <br><br />
[[Brown:Transformation Protocol with Fischer cells | '''Transformation Protocol with Fischer cells''']]<br> <br><br />
[[Brown:Gel Protocol | '''Gel Protocol''']]<br><br><br />
[[Brown:TAE solution Protocols| '''TAE solution Protocols''']]<br><br><br />
[[Brown:Protocols:Magnetotactic Bacteria]]</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Lab_ProtocolsBrown:Lab Protocols2006-07-07T14:04:36Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br> <br><br />
[[Brown:Transformation Protocol with our competent cells | '''Transformation Protocol with our competent cells''']]<br> <br><br />
[[Brown:Transformation Protocol with Fischer cells | '''Transformation Protocol with Fischer cells''']]<br> <br><br />
[[Brown:Gel Protocol | '''Gel Protocol''']]<br><br><br />
[[Brown:TAE solution Protocols| '''TAE solution Protocols''']]</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Lab_ProtocolsBrown:Lab Protocols2006-07-07T14:04:23Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br> <br><br />
[[Brown:Transformation Protocol with our competent cells | '''Transformation Protocol with our competent cells''']]<br> <br><br />
<br />
<br />
[[Brown:Transformation Protocol with Fischer cells | '''Transformation Protocol with Fischer cells''']]<br> <br><br />
<br />
<br />
[[Brown:Gel Protocol | '''Gel Protocol''']]<br><br><br />
[[Brown:TAE solution Protocols| '''TAE solution Protocols''']]</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Lab_ProtocolsBrown:Lab Protocols2006-07-07T14:03:54Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br> <br><br />
[[Brown:Transformation Protocol with our competent cells | '''Transformation Protocol with our competent cells''']]<br> <br><br />
<br />
<br />
[[Brown:Transformation Protocol with Fischer cells | '''Transformation Protocol with Fischer cells''']]<br> <br><br />
<br />
<br />
[[Brown:Gel Protocol | '''Gel Protocol''']]<br />
<br />
<br><br><br />
<br />
[[Brown:TAE solution Protocols| '''TAE solution Protocols''']]</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:TAE_solution_ProtocolsBrown:TAE solution Protocols2006-07-07T14:03:08Z<p>Agao: </p>
<hr />
<div>[[Brown:Lab Protocols| Back]]<br><br><br />
TAE SOLUTION PROTOCOL<br />
<br />
I. 50X TAE STOCK SOLUTION<br />
<br />
1. FOR EACH LITER OF SOLUTION:<br />
A.) 242 g. TRIS BASE<br />
B.) 57.1mL glacial acetic acid<br />
C.) 100mL 0.5M EDTA<br />
<br />
2. Mix Tris with stir bar to dissolve in about 600mL of ddH20<br />
<br />
3. Add EDTA and Acetic Acid<br />
<br />
4. Bring final volume to 1L with ddH20<br />
<br />
5. Store at room temp.<br />
<br />
II. 1X TAE PROTOCOL<br />
<br />
1. Add 10mL of 50X TAE to enough ddH2O to make 500mL solution</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:TAE_solution_ProtocolsBrown:TAE solution Protocols2006-07-07T14:02:56Z<p>Agao: </p>
<hr />
<div>[[Brown:Lab Protocols| Back]]<br><br />
TAE SOLUTION PROTOCOL<br />
<br />
I. 50X TAE STOCK SOLUTION<br />
<br />
1. FOR EACH LITER OF SOLUTION:<br />
A.) 242 g. TRIS BASE<br />
B.) 57.1mL glacial acetic acid<br />
C.) 100mL 0.5M EDTA<br />
<br />
2. Mix Tris with stir bar to dissolve in about 600mL of ddH20<br />
<br />
3. Add EDTA and Acetic Acid<br />
<br />
4. Bring final volume to 1L with ddH20<br />
<br />
5. Store at room temp.<br />
<br />
II. 1X TAE PROTOCOL<br />
<br />
1. Add 10mL of 50X TAE to enough ddH2O to make 500mL solution</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Lab_ProtocolsBrown:Lab Protocols2006-07-07T14:01:54Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br> <br><br />
[[Brown:Transformation Protocol with our competent cells | Transformation Protocol with our competent cells]]<br> <br><br />
<br />
<br />
[[Brown:Transformation Protocol with Fischer cells | Transformation Protocol with Fischer cells]]<br> <br><br />
<br />
<br />
[[Brown:Gel Protocol | Gel Protocol]]<br />
<br />
<br><br><br />
<br />
[[Brown:TAE solution Protocols| TAE solution Protocols]]</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Next_Meeting_AgendaBrown:Next Meeting Agenda2006-07-06T19:24:12Z<p>Agao: /* Friday July 7, 2006 */</p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br />
<br />
<br />
== Friday July 7, 2006 ==<br />
<br />
1. Check in from last week <br><br />
2. Progress on group projects <br><br />
3. Modeling? <br><br />
4. Update on magnetotactic bacteria <br><br />
5. Job delegations <br><br />
6. Cleaning schedule <br><br />
7. Use the wiki <br><br />
8. New business? <br></div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Next_Meeting_AgendaBrown:Next Meeting Agenda2006-07-06T19:23:10Z<p>Agao: /* Friday July 7, 2006 */</p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br />
<br />
<br />
== Friday July 7, 2006 ==<br />
<br />
1. Check in from last week <br><br />
2. Progress on group projects <br><br />
3. Update on magnetotactic bacteria <br><br />
4. Job delegations <br><br />
5. Use the wiki <br><br />
6. New business? <br></div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Next_Meeting_AgendaBrown:Next Meeting Agenda2006-07-06T19:22:29Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br />
<br />
<br />
== Friday July 7, 2006 ==<br />
<br />
I. Check in from last week <br><br />
II. Progress on group projects <br><br />
III. Update on magnetotactic bacteria <br><br />
IV. Job delegations <br><br />
V. Use the wiki <br></div>Agaohttp://2006.igem.org/wiki/index.php/User:AnniegaoUser:Anniegao2006-07-06T19:19:37Z<p>Agao: /* Weekly Reports */</p>
<hr />
<div>Year: 2009 <br><br />
Concentration: Biology<br><br />
Hometown: East Lyme, CT<br />
<br />
As a freshman, I didn't think that I would have many opportunities at participating in real lab research. So I was very excited to learn about the iGEM project both because of its cross-disciplinary involvement and because it offered me a chance to get involved in a real scientific research project. The birth of the iGEM team is just one example of how Brown offers its students the resources necessary for them to shape their own education.<br />
<br />
<br />
== Weekly Reports==<br />
<Br><br />
<br />
'''Week Four'''<Br>I helped to divide our new idea of the bacterial freeze tag into four smaller projects. Jamie Lemon and I began working on the project called the freeze machine. We successfully transformed all of the parts required for this project. We also began miniprepping our parts, but were unable to finish due to a shortage of miniprep columns. We will finish the minipreps next week and then proceed onto digestion and ligations.<br />
<br><br />
'''Week Five'''<br><br />
Wednesday: Performed transformations for Freeze machine parts. Made Amp. plates.<br />
Thursday: Updated wiki with Jamie Lemon and made of list of tasks for the group. We also grew up colonies for a miniprep tomorrow.</div>Agaohttp://2006.igem.org/wiki/index.php/User:AnniegaoUser:Anniegao2006-07-06T19:14:19Z<p>Agao: /* Weekly Reports */</p>
<hr />
<div>Year: 2009 <br><br />
Concentration: Biology<br><br />
Hometown: East Lyme, CT<br />
<br />
As a freshman, I didn't think that I would have many opportunities at participating in real lab research. So I was very excited to learn about the iGEM project both because of its cross-disciplinary involvement and because it offered me a chance to get involved in a real scientific research project. The birth of the iGEM team is just one example of how Brown offers its students the resources necessary for them to shape their own education.<br />
<br />
<br />
== Weekly Reports==<br />
<Br><br />
<br />
'''Week Four'''<Br>I helped to divide our new idea of the bacterial freeze tag into four smaller projects. Jamie Lemon and I began working on the project called the freeze machine. We successfully transformed all of the parts required for this project. We also began miniprepping our parts, but were unable to finish due to a shortage of miniprep columns. We will finish the minipreps next week and then proceed onto digestion and ligations.<br />
<br><br />
'''Week Five'''<br><br />
Wednesday: Performed transformations for Freeze machine parts. Made Amp. plates.</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Gel_ProtocolBrown:Gel Protocol2006-07-06T19:10:49Z<p>Agao: </p>
<hr />
<div>[[Brown:Lab Protocols |Back]] <br><br><br />
GEL PROTOCOL<br />
<br />
<br />
1. PUSH GEL TRAY DOWN INTO POURING BOX.<br />
<br />
2. PLACE COMB OF APPROPRIATE WIDTH, SIZE, AND NUMBER INTO NICHE AT THE END OF THE TRAY. (MAKE SURE IT’S AT THE END WHERE THE BLACK WIRE WILL CONNECT)<br />
<br />
3. POUR AGAROSE SOLUTION (1% AGAROSE IN 1X TAE), 40ML FOR SMALL GELS<br />
<br />
4. ADD 1µL OF ETHIDIUM BROMIDE PER 40mL OF SOLUTION<br />
<br />
5. SWIRL AND POUR MIXTURE INTO TRAY<br />
<br />
6. LET GEL SOLIDIFY</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Transformation_Protocol_with_Fischer_cellsBrown:Transformation Protocol with Fischer cells2006-07-06T19:09:50Z<p>Agao: </p>
<hr />
<div>[[Brown:Lab Protocols |Back]] <br><br><br />
TRANSFORMATION PROTOCOL WITH FISCHER COMPETENT CELLS<br />
<br />
<br />
1. TAKE CELLS FROM -80 AND THAW FOR IMMEDIATE USE<br />
<br />
2. MIX CELLS GENTLY WITH TAPPING<br />
<br />
3. ADD 4µL DNA TO 50µL COMPETENT CELLS (EACH VIAL CONTAINS 50µL)<br />
<br />
4. INCUBATE ON ICE FOR 30 MIN.<br />
<br />
5. HEAT SHOCK AT 42˚C FOR 30 SEC.<br />
<br />
6. PLACE ON ICE FOR 2 MIN.<br />
<br />
7. ADD 250µL CIRCLE GROW<br />
<br />
8. INCUBATE FOR 1 HOUR WITH SHAKING AT 37˚C<br />
<br />
9. ADD 250µL CIRLCE GROW<br />
<br />
10. PLATE 200µL OF MIX</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Transformation_Protocol_with_our_competent_cellsBrown:Transformation Protocol with our competent cells2006-07-06T19:09:19Z<p>Agao: </p>
<hr />
<div>[[Brown:Lab Protocols |Back]] <br><br><br />
TRANSFORMATION PROTOCOL (WITH OUR COMPETENT CELLS)<br />
<br />
1.) TAKE COMPETENT CELLS OUT OF THE -80 AND THAW (CAREFUL NOT TO THAW FOR TOO LONG)<br />
<br />
2.) ADD 25µL CELLS TO 75µL CALCIUM CHLORIDE IN EPENDORF TUBE<br />
<br />
3.) ADD 6µL DNA TO TUBE<br />
<br />
4.) INCUBATE ON ICE FOR 30 MIN.<br />
<br />
5.) HEAT SHOCK AT 42˚C FOR 1 MIN.<br />
<br />
6.) INCUBATE ON ICE FOR 2 MIN.<br />
<br />
7.) ADD 900µL LB/CIRCLE GROW<br />
<br />
8.) GROW AT 37˚C WITH SHAKING FOR 1 HOUR<br />
<br />
9.) PLATE 300µL OF MIX ONTO WARMED LB PLATES<br />
<br />
10.) GROW OVERNIGHT AT 37˚C</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Transformation_Protocol_with_our_competent_cellsBrown:Transformation Protocol with our competent cells2006-07-06T19:09:02Z<p>Agao: </p>
<hr />
<div>[[Brown:Lab Protocols |Back]] <br><br />
TRANSFORMATION PROTOCOL (WITH OUR COMPETENT CELLS)<br />
<br />
1.) TAKE COMPETENT CELLS OUT OF THE -80 AND THAW (CAREFUL NOT TO THAW FOR TOO LONG)<br />
<br />
2.) ADD 25µL CELLS TO 75µL CALCIUM CHLORIDE IN EPENDORF TUBE<br />
<br />
3.) ADD 6µL DNA TO TUBE<br />
<br />
4.) INCUBATE ON ICE FOR 30 MIN.<br />
<br />
5.) HEAT SHOCK AT 42˚C FOR 1 MIN.<br />
<br />
6.) INCUBATE ON ICE FOR 2 MIN.<br />
<br />
7.) ADD 900µL LB/CIRCLE GROW<br />
<br />
8.) GROW AT 37˚C WITH SHAKING FOR 1 HOUR<br />
<br />
9.) PLATE 300µL OF MIX ONTO WARMED LB PLATES<br />
<br />
10.) GROW OVERNIGHT AT 37˚C</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Transformation_Protocol_with_our_competent_cellsBrown:Transformation Protocol with our competent cells2006-07-06T19:08:25Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]] <br><br />
TRANSFORMATION PROTOCOL (WITH OUR COMPETENT CELLS)<br />
<br />
1.) TAKE COMPETENT CELLS OUT OF THE -80 AND THAW (CAREFUL NOT TO THAW FOR TOO LONG)<br />
<br />
2.) ADD 25µL CELLS TO 75µL CALCIUM CHLORIDE IN EPENDORF TUBE<br />
<br />
3.) ADD 6µL DNA TO TUBE<br />
<br />
4.) INCUBATE ON ICE FOR 30 MIN.<br />
<br />
5.) HEAT SHOCK AT 42˚C FOR 1 MIN.<br />
<br />
6.) INCUBATE ON ICE FOR 2 MIN.<br />
<br />
7.) ADD 900µL LB/CIRCLE GROW<br />
<br />
8.) GROW AT 37˚C WITH SHAKING FOR 1 HOUR<br />
<br />
9.) PLATE 300µL OF MIX ONTO WARMED LB PLATES<br />
<br />
10.) GROW OVERNIGHT AT 37˚C</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Lab_ProtocolsBrown:Lab Protocols2006-07-06T19:06:47Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br> <br><br />
[[Brown:Transformation Protocol with our competent cells | Transformation Protocol with our competent cells]]<br> <br><br />
<br />
<br />
[[Brown:Transformation Protocol with Fischer cells | Transformation Protocol with Fischer cells]]<br> <br><br />
<br />
<br />
[[Brown:Gel Protocol | Gel Protocol]]</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Lab_ProtocolsBrown:Lab Protocols2006-07-06T19:06:31Z<p>Agao: </p>
<hr />
<div>[[Brown:iGEM portal 2006 |Home]]<br />
[[Brown:Transformation Protocol with our competent cells | Transformation Protocol with our competent cells]]<br> <br><br />
<br />
<br />
[[Brown:Transformation Protocol with Fischer cells | Transformation Protocol with Fischer cells]]<br> <br><br />
<br />
<br />
[[Brown:Gel Protocol | Gel Protocol]]</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Transformation_Protocol_with_our_competent_cellsBrown:Transformation Protocol with our competent cells2006-07-06T19:05:56Z<p>Agao: </p>
<hr />
<div>TRANSFORMATION PROTOCOL (WITH OUR COMPETENT CELLS)<br />
<br />
1.) TAKE COMPETENT CELLS OUT OF THE -80 AND THAW (CAREFUL NOT TO THAW FOR TOO LONG)<br />
<br />
2.) ADD 25µL CELLS TO 75µL CALCIUM CHLORIDE IN EPENDORF TUBE<br />
<br />
3.) ADD 6µL DNA TO TUBE<br />
<br />
4.) INCUBATE ON ICE FOR 30 MIN.<br />
<br />
5.) HEAT SHOCK AT 42˚C FOR 1 MIN.<br />
<br />
6.) INCUBATE ON ICE FOR 2 MIN.<br />
<br />
7.) ADD 900µL LB/CIRCLE GROW<br />
<br />
8.) GROW AT 37˚C WITH SHAKING FOR 1 HOUR<br />
<br />
9.) PLATE 300µL OF MIX ONTO WARMED LB PLATES<br />
<br />
10.) GROW OVERNIGHT AT 37˚C</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:Lab_ProtocolsBrown:Lab Protocols2006-07-06T19:04:59Z<p>Agao: </p>
<hr />
<div><br />
[[Brown:Transformation Protocol with our competent cells | Transformation Protocol with our competent cells]]<br> <br><br />
<br />
<br />
[[Brown:Transformation Protocol with Fischer cells | Transformation Protocol with Fischer cells]]<br> <br><br />
<br />
<br />
[[Brown:Gel Protocol | Gel Protocol]]</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:iGEM_portal_2006Brown:iGEM portal 20062006-07-06T19:01:49Z<p>Agao: </p>
<hr />
<div>{{Brown navigation bar}}<br />
<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0; width: 750px; color: #000000; background-color: #ffffff;"<br />
| width=717px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<h3>Welcome</h3><br />
Welcome to the Brown [http://icampus.mit.edu/projects/iGem.shtml International Genetically Engineered Machine (iGEM)] Team Wiki. Our goal is to participate in the iGEM Competition for the summer of 2006. Feel free to explore our website or [[Brown:Contact | contact us]], we are constantly updating it with the latest developments in the project. <br />
<br />
<h3>Team news </h3><br />
6/18/06 - Funded students - remember you need to add [[User:Johncumbers| a weekly report]] to your user page <br><br />
6/18/06 - Updated [[Brown:Calendar pages |calendar page]], now all info on single page <br><br />
4/22/06 - Please pick up some tasks from the new [[Brown:Todo list | To do list]] <br><br />
3/29/06 - Updated [[Brown:Roles/future planning| Future planning]] section <br><br />
3/29/06 - Please make sure you have signed yourself up for the [[Brown:Journal club:Synthetic biology journal club |journal club]] <br><br />
3/29/06 - If you are in charge of one of the roles listed below, please update your section with your ideas and plans<br />
<br />
|}<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>Team roles</h3><br />
<br />
Team Leaders: [[user:jamielemon|Jamie L.]], [[user:anniegao|Annie]]<br><br />
Fundraising: <br><br />
Secretary: <br><br />
Journal Club Coordinator: [[user:petergoldstein|Peter]] <br><br />
Faculty Liason: [[user:azeemkaka| Azeem]]<br><br />
Industry Liason: <br> <br />
[[Brown:Roles/future planning| Future planning]]: [[user:johncumbers|John]] <br> <br />
Chemical Database Manager: [[user:petergoldstein|Peter]] <br><br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>[[Brown:Todo list |Todo list]]</h3><br />
<h3>[[Brown:Lab Protocols| Lab Protocols]] </h3><br />
<h3>[[Brown:Next Meeting Agenda |Next Meeting Agenda ]]</h3><br />
<br />
<h3>[[Brown:Equipment needed|Equipment needed]]</h3><br />
<br />
<h3>[[Brown:Summer workshops|Summer Workshops]]</h3><br />
<br />
<h3>[[Brown:Calendar pages|Calendar Pages]]</h3><br />
<br />
<h3>[[Brown:Projects |Projects]]</h3><br />
<br />
<h3>Research</h3><br />
#[[Brown:Journal_club:Synthetic_biology_journal_club|Journal Club]]<br />
#[http://www.cumbers.com/igem Idea factory] <br><br />
#[[Brown:Protocols|Protocols]]<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>About Us</h3><br />
#[[Brown:Lab Members | Faculty Advisors]]<br />
#[[Brown:Lab Members | Team Members]]<br />
#[[Brown:Industry Affiliates | Industry Affiliates]]<br />
#[[Brown:March142006 | Meeting minutes]]<br />
<br />
Our lab is in J Walter Wilson, Rm 228, call 401 523 8190<br />
<br />
__NOTOC__</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:iGEM_portal_2006Brown:iGEM portal 20062006-07-06T17:42:40Z<p>Agao: </p>
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<h3>Welcome</h3><br />
Welcome to the Brown [http://icampus.mit.edu/projects/iGem.shtml International Genetically Engineered Machine (iGEM)] Team Wiki. Our goal is to participate in the iGEM Competition for the summer of 2006. Feel free to explore our website or [[Brown:Contact | contact us]], we are constantly updating it with the latest developments in the project. <br />
<br />
<h3>Team news </h3><br />
6/18/06 - Funded students - remember you need to add [[User:Johncumbers| a weekly report]] to your user page <br><br />
6/18/06 - Updated [[Brown:Calendar pages |calendar page]], now all info on single page <br><br />
4/22/06 - Please pick up some tasks from the new [[Brown:Todo list | To do list]] <br><br />
3/29/06 - Updated [[Brown:Roles/future planning| Future planning]] section <br><br />
3/29/06 - Please make sure you have signed yourself up for the [[Brown:Journal club:Synthetic biology journal club |journal club]] <br><br />
3/29/06 - If you are in charge of one of the roles listed below, please update your section with your ideas and plans<br />
<br />
|}<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>Team roles</h3><br />
<br />
Team Leaders: [[user:jamielemon|Jamie L.]], [[user:anniegao|Annie]]<br><br />
Fundraising: <br><br />
Secretary: <br><br />
Journal Club Coordinator: [[user:petergoldstein|Peter]] <br><br />
Faculty Liason: [[user:azeemkaka| Azeem]]<br><br />
Industry Liason: <br> <br />
[[Brown:Roles/future planning| Future planning]]: [[user:johncumbers|John]] <br> <br />
Chemical Database Manager: [[user:petergoldstein|Peter]] <br><br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>[[Brown:Todo list |Todo list]]</h3><br />
<h3>[[Brown:Next Meeting Agenda |Next Meeting Agenda ]]</h3><br />
<br />
<h3>[[Brown:Equipment needed|Equipment needed]]</h3><br />
<br />
<h3>[[Brown:Summer workshops|Summer Workshops]]</h3><br />
<br />
<h3>[[Brown:Calendar pages|Calendar Pages]]</h3><br />
<br />
<h3>[[Brown:Projects |Projects]]</h3><br />
<br />
<h3>Research</h3><br />
#[[Brown:Journal_club:Synthetic_biology_journal_club|Journal Club]]<br />
#[http://www.cumbers.com/igem Idea factory] <br><br />
#[[Brown:Protocols|Protocols]]<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>About Us</h3><br />
#[[Brown:Lab Members | Faculty Advisors]]<br />
#[[Brown:Lab Members | Team Members]]<br />
#[[Brown:Industry Affiliates | Industry Affiliates]]<br />
#[[Brown:March142006 | Meeting minutes]]<br />
<br />
Our lab is in J Walter Wilson, Rm 228, call 401 523 8190<br />
<br />
__NOTOC__</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:iGEM_portal_2006Brown:iGEM portal 20062006-07-06T17:42:18Z<p>Agao: </p>
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{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0; width: 750px; color: #000000; background-color: #ffffff;"<br />
| width=717px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<h3>Welcome</h3><br />
Welcome to the Brown [http://icampus.mit.edu/projects/iGem.shtml International Genetically Engineered Machine (iGEM)] Team Wiki. Our goal is to participate in the iGEM Competition for the summer of 2006. Feel free to explore our website or [[Brown:Contact | contact us]], we are constantly updating it with the latest developments in the project. <br />
<br />
<h3>Team news </h3><br />
6/18/06 - Funded students - remember you need to add [[User:Johncumbers| a weekly report]] to your user page <br><br />
6/18/06 - Updated [[Brown:Calendar pages |calendar page]], now all info on single page <br><br />
4/22/06 - Please pick up some tasks from the new [[Brown:Todo list | To do list]] <br><br />
3/29/06 - Updated [[Brown:Roles/future planning| Future planning]] section <br><br />
3/29/06 - Please make sure you have signed yourself up for the [[Brown:Journal club:Synthetic biology journal club |journal club]] <br><br />
3/29/06 - If you are in charge of one of the roles listed below, please update your section with your ideas and plans<br />
<br />
|}<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>Team roles</h3><br />
<br />
Team Leaders: [[user:jamielemon|Jamie L.]], [[user:anniegao|Annie]]<br><br />
Fundraising: <br><br />
Secretary: <br><br />
Journal Club Coordinator: [[user:petergoldstein|Peter]] <br><br />
Faculty liason: [[user:azeemkaka| Azeem]]<br><br />
Industry liason: <br> <br />
[[Brown:Roles/future planning| Future planning]]: [[user:johncumbers|John]] <br> <br />
Chemical Database Manager: [[user:petergoldstein|Peter]] <br><br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>[[Brown:Todo list |Todo list]]</h3><br />
<h3>[[Brown:Next Meeting Agenda |Next Meeting Agenda ]]</h3><br />
<br />
<h3>[[Brown:Equipment needed|Equipment needed]]</h3><br />
<br />
<h3>[[Brown:Summer workshops|Summer Workshops]]</h3><br />
<br />
<h3>[[Brown:Calendar pages|Calendar Pages]]</h3><br />
<br />
<h3>[[Brown:Projects |Projects]]</h3><br />
<br />
<h3>Research</h3><br />
#[[Brown:Journal_club:Synthetic_biology_journal_club|Journal Club]]<br />
#[http://www.cumbers.com/igem Idea factory] <br><br />
#[[Brown:Protocols|Protocols]]<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>About Us</h3><br />
#[[Brown:Lab Members | Faculty Advisors]]<br />
#[[Brown:Lab Members | Team Members]]<br />
#[[Brown:Industry Affiliates | Industry Affiliates]]<br />
#[[Brown:March142006 | Meeting minutes]]<br />
<br />
Our lab is in J Walter Wilson, Rm 228, call 401 523 8190<br />
<br />
__NOTOC__</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:iGEM_portal_2006Brown:iGEM portal 20062006-07-06T17:40:06Z<p>Agao: </p>
<hr />
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{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0; width: 750px; color: #000000; background-color: #ffffff;"<br />
| width=717px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<h3>Welcome</h3><br />
Welcome to the Brown [http://icampus.mit.edu/projects/iGem.shtml International Genetically Engineered Machine (iGEM)] Team Wiki. Our goal is to participate in the iGEM Competition for the summer of 2006. Feel free to explore our website or [[Brown:Contact | contact us]], we are constantly updating it with the latest developments in the project. <br />
<br />
<h3>Team news </h3><br />
6/18/06 - Funded students - remember you need to add [[User:Johncumbers| a weekly report]] to your user page <br><br />
6/18/06 - Updated [[Brown:Calendar pages |calendar page]], now all info on single page <br><br />
4/22/06 - Please pick up some tasks from the new [[Brown:Todo list | To do list]] <br><br />
3/29/06 - Updated [[Brown:Roles/future planning| Future planning]] section <br><br />
3/29/06 - Please make sure you have signed yourself up for the [[Brown:Journal club:Synthetic biology journal club |journal club]] <br><br />
3/29/06 - If you are in charge of one of the roles listed below, please update your section with your ideas and plans<br />
<br />
|}<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>Team roles</h3><br />
<br />
Team Leaders: [[user:jamielemon|Jamie L.]], [[user:anniegao|Annie]]<br><br />
Fundraising: [[user:juliespector|Julie]], [[user:Sheldonprovost|Sheldon Provost]] <br><br />
Secretary: [[user:angelawong|Angela]] <br><br />
Journal Club Coordinator: [[user:petergoldstein|Peter]] <br><br />
Faculty liason: [[user:azeemkaka| Azeem]]<br><br />
Industry liason <br> <br />
[[Brown:Roles/future planning| Future planning]]: [[user:johncumbers|John]] <br> <br />
Chemical Database Manager: [[user:petergoldstein|Peter]] <br><br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>[[Brown:Todo list |Todo list]]</h3><br />
<h3>[[Brown:Next Meeting Agenda |Next Meeting Agenda ]]</h3><br />
<br />
<h3>[[Brown:Equipment needed|Equipment needed]]</h3><br />
<br />
<h3>[[Brown:Summer workshops|Summer Workshops]]</h3><br />
<br />
<h3>[[Brown:Calendar pages|Calendar Pages]]</h3><br />
<br />
<h3>[[Brown:Projects |Projects]]</h3><br />
<br />
<h3>Research</h3><br />
#[[Brown:Journal_club:Synthetic_biology_journal_club|Journal Club]]<br />
#[http://www.cumbers.com/igem Idea factory] <br><br />
#[[Brown:Protocols|Protocols]]<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>About Us</h3><br />
#[[Brown:Lab Members | Faculty Advisors]]<br />
#[[Brown:Lab Members | Team Members]]<br />
#[[Brown:Industry Affiliates | Industry Affiliates]]<br />
#[[Brown:March142006 | Meeting minutes]]<br />
<br />
Our lab is in J Walter Wilson, Rm 228, call 401 523 8190<br />
<br />
__NOTOC__</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:iGEM_portal_2006Brown:iGEM portal 20062006-07-06T17:39:10Z<p>Agao: </p>
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{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0; width: 750px; color: #000000; background-color: #ffffff;"<br />
| width=717px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<h3>Welcome</h3><br />
Welcome to the Brown [http://icampus.mit.edu/projects/iGem.shtml International Genetically Engineered Machine (iGEM)] Team Wiki. Our goal is to participate in the iGEM Competition for the summer of 2006. Feel free to explore our website or [[Brown:Contact | contact us]], we are constantly updating it with the latest developments in the project. <br />
<br />
<h3>Team news </h3><br />
6/18/06 - Funded students - remember you need to add [[User:Johncumbers| a weekly report]] to your user page <br><br />
6/18/06 - Updated [[Brown:Calendar pages |calendar page]], now all info on single page <br><br />
4/22/06 - Please pick up some tasks from the new [[Brown:Todo list | To do list]] <br><br />
3/29/06 - Updated [[Brown:Roles/future planning| Future planning]] section <br><br />
3/29/06 - Please make sure you have signed yourself up for the [[Brown:Journal club:Synthetic biology journal club |journal club]] <br><br />
3/29/06 - If you are in charge of one of the roles listed below, please update your section with your ideas and plans<br />
<br />
|}<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>Team roles</h3><br />
<br />
Team Leaders: [[user:jamielemon|Jamie L.]], [[user:anniegao|Annie]]<br><br />
Fundraising: [[user:juliespector|Julie]], [[user:Sheldonprovost|Sheldon Provost]] <br><br />
Secretary: [[user:angelawong|Angela]] <br><br />
Journal Club Coordinator: [[user:petergoldstein|Peter]] <br><br />
Faculty liason: [[user:azeemkaka| Azeem]]<br><br />
Industry liason <br> <br />
[[Brown:Roles/future planning| Future planning]]: [[user:johncumbers|John]] <br> <br />
Chemical Database Manager: [[user:petergoldstein|Peter]] <br><br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>[[Brown:Todo list |Todo list]]</h3><br />
<h3>[[Brown:Next Meeting Agenda |Next Meeting Agenda ]]</h3><br />
<br />
<br />
<h3>[[Brown:Equipment needed|Equipment needed]]</h3><br />
<br />
<h3>[[Brown:Summer workshops|Summer Workshops]]</h3><br />
<br />
<h3>[[Brown:Calendar pages|Calendar Pages]]</h3><br />
<br />
<h3>[[Brown:Projects |Projects]]</h3><br />
<br />
<h3>Research</h3><br />
#[[Brown:Journal_club:Synthetic_biology_journal_club|Journal Club]]<br />
#[http://www.cumbers.com/igem Idea factory] <br><br />
#[[Brown:Protocols|Protocols]]<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>About Us</h3><br />
#[[Brown:Lab Members | Faculty Advisors]]<br />
#[[Brown:Lab Members | Team Members]]<br />
#[[Brown:Industry Affiliates | Industry Affiliates]]<br />
#[[Brown:March142006 | Meeting minutes]]<br />
<br />
Our lab is in J Walter Wilson, Rm 228, call 401 523 8190<br />
<br />
__NOTOC__</div>Agaohttp://2006.igem.org/wiki/index.php/Brown:iGEM_portal_2006Brown:iGEM portal 20062006-07-06T17:38:24Z<p>Agao: </p>
<hr />
<div>{{Brown navigation bar}}<br />
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{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0; width: 750px; color: #000000; background-color: #ffffff;"<br />
| width=717px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<h3>Welcome</h3><br />
Welcome to the Brown [http://icampus.mit.edu/projects/iGem.shtml International Genetically Engineered Machine (iGEM)] Team Wiki. Our goal is to participate in the iGEM Competition for the summer of 2006. Feel free to explore our website or [[Brown:Contact | contact us]], we are constantly updating it with the latest developments in the project. <br />
<br />
<h3>Team news </h3><br />
6/18/06 - Funded students - remember you need to add [[User:Johncumbers| a weekly report]] to your user page <br><br />
6/18/06 - Updated [[Brown:Calendar pages |calendar page]], now all info on single page <br><br />
4/22/06 - Please pick up some tasks from the new [[Brown:Todo list | To do list]] <br><br />
3/29/06 - Updated [[Brown:Roles/future planning| Future planning]] section <br><br />
3/29/06 - Please make sure you have signed yourself up for the [[Brown:Journal club:Synthetic biology journal club |journal club]] <br><br />
3/29/06 - If you are in charge of one of the roles listed below, please update your section with your ideas and plans<br />
<br />
|}<br />
{| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;"<br />
|-valign="top"<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>Team roles</h3><br />
<br />
Team Leaders: [[user:jamielemon|Jamie L.]], [[user:anniegao|Annie]]<br><br />
Fundraising: [[user:juliespector|Julie]], [[user:Sheldonprovost|Sheldon Provost]] <br><br />
Secretary: [[user:angelawong|Angela]] <br><br />
Journal Club Coordinator: [[user:petergoldstein|Peter]] <br><br />
Faculty liason: [[user:azeemkaka| Azeem]]<br><br />
Industry liason <br> <br />
[[Brown:Roles/future planning| Future planning]]: [[user:johncumbers|John]] <br> <br />
Chemical Database Manager: [[user:petergoldstein|Peter]] <br><br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>[[Brown:Todo list |Todo list]]</h3><br />
<h3>[[Brown:Next Meeting Agenda |Next Meeting Agenda ]]</h3><br />
<h3>[[Brown:Roles/future planning|Roles/future planning]]</h3><br />
<br />
<h3>[[Brown:Equipment needed|Equipment needed]]</h3><br />
<br />
<h3>[[Brown:Summer workshops|Summer Workshops]]</h3><br />
<br />
<h3>[[Brown:Calendar pages|Calendar Pages]]</h3><br />
<br />
<h3>[[Brown:Projects |Projects]]</h3><br />
<br />
<h3>Research</h3><br />
#[[Brown:Journal_club:Synthetic_biology_journal_club|Journal Club]]<br />
#[http://www.cumbers.com/igem Idea factory] <br><br />
#[[Brown:Protocols|Protocols]]<br />
|width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" |<br />
<br />
<h3>About Us</h3><br />
#[[Brown:Lab Members | Faculty Advisors]]<br />
#[[Brown:Lab Members | Team Members]]<br />
#[[Brown:Industry Affiliates | Industry Affiliates]]<br />
#[[Brown:March142006 | Meeting minutes]]<br />
<br />
Our lab is in J Walter Wilson, Rm 228, call 401 523 8190<br />
<br />
__NOTOC__</div>Agao