Third Meeting, Wednesday, August 9, 2006
From 2006.igem.org
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4 basic endpoints: RFP, GFP, LacI and cI. Thus, the thermometer will be divided up into 4 basic modules. | 4 basic endpoints: RFP, GFP, LacI and cI. Thus, the thermometer will be divided up into 4 basic modules. | ||
| - | Considerations: | + | <b>Considerations:</b> |
<ul> | <ul> | ||
<li>Try to make parts that can be used to build other parts later (ie. Make a part and if test is | <li>Try to make parts that can be used to build other parts later (ie. Make a part and if test is | ||
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constructed, the first module will be more thoroughly tested for linear range, transcription | constructed, the first module will be more thoroughly tested for linear range, transcription | ||
rates, etc. | rates, etc. | ||
| + | <li>All functionality of the parts is in context. One part may work fine in one context but not | ||
| + | work in the next | ||
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</ul> | </ul> | ||
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''[[http://2006.igem.org/Critical_Design_Review back]]'' | ''[[http://2006.igem.org/Critical_Design_Review back]]'' | ||
Revision as of 02:29, 16 August 2006
The Third Critical Design Review meeting took on 9th August, Wednesday at the SF Basement(SUDS).
Overview
4 basic endpoints: RFP, GFP, LacI and cI. Thus, the thermometer will be divided up into 4 basic modules.
Considerations:
- Try to make parts that can be used to build other parts later (ie. Make a part and if test is successful, the part can then be used as is in the next ligation)
- Parallel Processing is KEY. Need to build as many different parts at the same time in order to use our time efficiently. We do not have time to wait and see if one part works before moving onto the next one. On that note, we will probably have to do at least two replicates per part (done by two different people) so that we have at least two cracks at each part
- Once a module is completed, there will first be a cursory yes/no test to see if SOME output is produced. If yes, move onto building the second module. While the second module is being constructed, the first module will be more thoroughly tested for linear range, transcription rates, etc.
- All functionality of the parts is in context. One part may work fine in one context but not work in the next
