University of California Berkeley 2006

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[[berk2006dummypage]]
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<h1>Addressable Conjugation in Bacterial Networks</h1>
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[[Image:Berkeley.jpg|left|200px]]
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[[Image:Berkeley2006School.jpg|left|200px]]Networks of interacting cells provide the basis for neural learning.  We have developed the process of addressable conjugation for communication within a network of E. coli bacteria.  Here, bacteria send messages to one another via conjugation of plasmid DNAs, but the message is only meaningful to cells with a matching address sequence. In this way, the Watson Crick base-pairing of addressing sequences replaces the spatial connectivity present in neural systems. To construct this system, we have adapted natural conjugation systems as the communication device. Information contained in the transferred plasmids is only accessable by "unlocking" the message using RNA based 'keys'. The resulting addressable conjugation process is being adapted to construct a network of NAND logic gates in bacterial cultures. Ultimately, this will allow us to develop networks of bacteria capable of trained learning.<br>
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{| cellspacing="5"
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|width="180px"|
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<span style='font-size:12.0pt'>Our Team</span>
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<hr/>
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'''High School'''
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=='''Addressable Conjugation in Bacterial Networks'''==
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Matt Fleming<br>
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Kaitlin A. Davis<br>
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'''Undergrads'''
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Will Bosworth <br>
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Bryan Hernandez<br>
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Kaitlin A. Davis <br>
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Jennifer Lu<br>
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Matt Fleming <br>
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Samantha Liang<br>
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Bryan Hernandez <br>
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Daniel Kluesing<br>
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Daniel Kluesing <br>
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Will Bosworth<br>
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Samantha Liang <br>
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Jennifer Lu <br>
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J. Christopher Anderson <br>
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John E. Dueber <br>
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Adam P. Arkin <br>
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Jay D. Keasling <br>
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'''Postdocs'''
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Networks of interacting cells provide the basis for neural learning.  We have developed the process of addressable conjugation for communication within a network of E. coli bacteria.  Here, bacteria send messages to one another via conjugation of plasmid DNAs, but the message is only meaningful to cells with a matching address sequence. In this way, the Watson Crick base-pairing of addressing sequences replaces the spatial connectivity present in neural systems. To construct this system, we have adapted natural conjugation systems as the communication device. Information contained in the transferred plasmids is only accessable by "unlocking" the message using RNA based 'keys'. The resulting addressable conjugation process is being adapted to construct a network of NAND logic gates in bacterial cultures. Ultimately, this will allow us to develop networks of bacteria capable of trained learning.
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John E. Dueber<br>
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J. Christopher Anderson<br>
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The specific Aims of our project were:
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'''Faculty Advisors'''
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    1) Construct efficient riboregulator lock/key pairs
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    2) Control ***conjugation**
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    3) Show ***them together***
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[[Berkeley2006-RiboregulatorsMain | Riboregulators]] <br>
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Adam P. Arkin<br>
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[[Berkeley2006-ConjugationMain | Conjugation]] <br>
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Jay D. Keasling<br>
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[[Berkeley2006-NandMain | NAND]]<br>
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|width="20px"|
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[[Berkeley2006-NetworksMain | Networks]] <br>
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|width="600px"|
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[[Berkeley2006-PromoterMain | Promoter Engineering]] <br>
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<span style='font-size:12.0pt'>
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'''The specific goals of our project were to:'''<br>
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<span style='font-size:12.0pt'>
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[[Image:Berkeley2006IgemRocks.jpg | 60px]]'''Construct high-performance riboregulator pairs'''<br>
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[[Image:Berkeley2006IgemRocks.jpg | 60px]]'''Harness the process of bacterial conjugation'''<br>
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[[Image:Berkeley2006IgemRocks.jpg | 60px]]'''Demonstrate transmission of a coded message'''<br>
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[[Image:Berkeley2006IgemRocks.jpg | 60px]]'''Develop promoter tools to tune gene expression'''<br>
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</span>
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<hr/>
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To learn more about our system, follow the series of descriptions below:
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<span style='font-size:15.0pt'>
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[[Berkeley2006-RiboregulatorsMain | High-performance Riboregulators]] <br>
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<span style='font-size:15.0pt'>
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[[Berkeley2006-ConjugationMain | Harnessing Bacterial Conjugation]] <br>
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Visit our website at:
 
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[http://openwetware.org/wiki/IGEM:UC_Berkeley/2006 Berkeley 2006 iGEM]
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<span style='font-size:15.0pt'>
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[[Berkeley2006-NandMain | NAND Logic in Cellular Networks]]<br>
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<span style='font-size:15.0pt'>
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[[Berkeley2006-NetworksMain | Extending to Cellular Networks]] <br>
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<span style='font-size:15.0pt'>
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[[Berkeley2006-PromoterMain | Tuning Constitutive Expression Levels]] <br>
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|}
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----
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Visit our working website at  [http://openwetware.org/wiki/IGEM:UC_Berkeley/2006 Berkeley 2006 iGEM]

Revision as of 03:55, 28 October 2006

Addressable Conjugation in Bacterial Networks

Berkeley2006School.jpg
Networks of interacting cells provide the basis for neural learning. We have developed the process of addressable conjugation for communication within a network of E. coli bacteria. Here, bacteria send messages to one another via conjugation of plasmid DNAs, but the message is only meaningful to cells with a matching address sequence. In this way, the Watson Crick base-pairing of addressing sequences replaces the spatial connectivity present in neural systems. To construct this system, we have adapted natural conjugation systems as the communication device. Information contained in the transferred plasmids is only accessable by "unlocking" the message using RNA based 'keys'. The resulting addressable conjugation process is being adapted to construct a network of NAND logic gates in bacterial cultures. Ultimately, this will allow us to develop networks of bacteria capable of trained learning.

Our Team

High School

Matt Fleming
Kaitlin A. Davis

Undergrads

Bryan Hernandez
Jennifer Lu
Samantha Liang
Daniel Kluesing
Will Bosworth

Postdocs

John E. Dueber
J. Christopher Anderson

Faculty Advisors

Adam P. Arkin
Jay D. Keasling

The specific goals of our project were to:

Berkeley2006IgemRocks.jpgConstruct high-performance riboregulator pairs
Berkeley2006IgemRocks.jpgHarness the process of bacterial conjugation
Berkeley2006IgemRocks.jpgDemonstrate transmission of a coded message
Berkeley2006IgemRocks.jpgDevelop promoter tools to tune gene expression

To learn more about our system, follow the series of descriptions below:

High-performance Riboregulators


Harnessing Bacterial Conjugation


NAND Logic in Cellular Networks


Extending to Cellular Networks


Tuning Constitutive Expression Levels

Visit our working website at Berkeley 2006 iGEM

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