BU06:Team D
From 2006.igem.org
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== Current Progress == | == Current Progress == | ||
+ | ===Silent Point Mutation: XbaI at bp 2411 of P. luminescens LuxCDABE=== | ||
+ | _/_____ <---XbaI cut | ||
+ | 5' AAT CTA GAT 3' | ||
+ | N L D | ||
+ | Asn Leu Asp | ||
+ | |||
+ | Triplets for these A.A.'s | ||
+ | N L D | ||
+ | AAT TTA GAT | ||
+ | AAC TTG GAC | ||
+ | CTG | ||
+ | CTT | ||
+ | CTC | ||
+ | CTA | ||
+ | |||
+ | Codon Usage Bias! (Frequency: per thousand) | ||
+ | Leu Codon E.coliK12 P.lumin | ||
+ | CUU 11.0 12.1 | ||
+ | CUC 11.0 9.1 | ||
+ | CUA 3.9 9.0 | ||
+ | CUG 52.8 29.6 | ||
+ | CUA in P.lum is 9.0/1000, for a silent point mutation of Leucine, CUU or CUC with frequency of 11.0/1000... | ||
+ | and its still Leu!!!! | ||
Revision as of 19:04, 26 June 2006
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Contents |
Meeting Schedule
Current Progress
Silent Point Mutation: XbaI at bp 2411 of P. luminescens LuxCDABE
_/_____ <---XbaI cut 5' AAT CTA GAT 3' N L D Asn Leu Asp
Triplets for these A.A.'s N L D AAT TTA GAT AAC TTG GAC CTG CTT CTC CTA
Codon Usage Bias! (Frequency: per thousand) Leu Codon E.coliK12 P.lumin CUU 11.0 12.1 CUC 11.0 9.1 CUA 3.9 9.0 CUG 52.8 29.6
CUA in P.lum is 9.0/1000, for a silent point mutation of Leucine, CUU or CUC with frequency of 11.0/1000... and its still Leu!!!!
References
Techniques