SuperParts

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Parts List
Parts List
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pLac promoter -- BBa_R0010  DNA-1  7K
pLac promoter -- BBa_R0010  DNA-1  7K
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pAra promoter -- BBa_I13453  DNA-2  13D
pAra promoter -- BBa_I13453  DNA-2  13D
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RBS -- BBa_B0030  DNA-1  3G
RBS -- BBa_B0030  DNA-1  3G
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HixC – BBa_B?????
HixC – BBa_B?????
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double forward terminator -- BBa_B0015  DNA-1 1I
double forward terminator -- BBa_B0015  DNA-1 1I
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double backward terminator – BBa_B0025  DNA-1  3E
double backward terminator – BBa_B0025  DNA-1  3E
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KanR – BBa_B?????
KanR – BBa_B?????
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Recombination enhancer -- BBa_J3101  (Davidson)
Recombination enhancer -- BBa_J3101  (Davidson)
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Hin -- BBa_J31000  (Davidson)
Hin -- BBa_J31000  (Davidson)
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pAra promoter reverse – BBa_B?????  (Western)
pAra promoter reverse – BBa_B?????  (Western)
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RBS reverse – BBa_B?????  (Western)
RBS reverse – BBa_B?????  (Western)
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[[Image:Diamond1&2JPEG.jpg]]
[[Image:Diamond1&2JPEG.jpg]]
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Upon receipt of the TT-RE from Davidson, Western will construct Diamond 3 as below and complete final assembly for Construct 1 and Construct 1 – Control.
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[[Image:Diamond3&Constructs1JPEG.jpg]]
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[[Image:Constructs1JPEG.jpg]]

Latest revision as of 00:52, 30 June 2006

Western Assembly Plan

Parts List

pLac promoter -- BBa_R0010 DNA-1 7K

pAra promoter -- BBa_I13453 DNA-2 13D

RBS -- BBa_B0030 DNA-1 3G

HixC – BBa_B?????

double forward terminator -- BBa_B0015 DNA-1 1I

double backward terminator – BBa_B0025 DNA-1 3E

KanR – BBa_B?????

Recombination enhancer -- BBa_J3101 (Davidson)

Hin -- BBa_J31000 (Davidson)

pAra promoter reverse – BBa_B????? (Western)

RBS reverse – BBa_B????? (Western)

Upon receipt of the Hin and HixC from Davidson, Western will assemble the following Hin Expression Unit, both with and without an additional HixC, and send some of the finished product to Davidson (specifically that without the HixC site).


HinExpressionUnitJPEG.jpg

Western will also assemble Superparts necessary for the 1-pancake constructs in which the pAra promoter is both forward and backward. See diagram below.

SuperPartA1JPEG.jpg

SuperPartA2JPEG.jpg

SuperPartBJPEG.jpg

Diamond1&2JPEG.jpg

Upon receipt of the TT-RE from Davidson, Western will construct Diamond 3 as below and complete final assembly for Construct 1 and Construct 1 – Control.

Diamond3&Constructs1JPEG.jpg

Constructs1JPEG.jpg

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