Testing Results
From 2006.igem.org
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| - | We decided to integrate check-points into our construction plan, in order to test intermediary part functionality. There are three basic modules: | + | We decided to integrate check-points into our construction plan, in order to test intermediary part functionality. |
| + | <br>There are three basic modules: | ||
<ul> | <ul> | ||
<li>'''Module 1:''' Arabinose inducible pBad/araC promoter (I0500) produces temperature-sensitive | <li>'''Module 1:''' Arabinose inducible pBad/araC promoter (I0500) produces temperature-sensitive | ||
Revision as of 05:43, 13 October 2006
We decided to integrate check-points into our construction plan, in order to test intermediary part functionality.
There are three basic modules:
- Module 1: Arabinose inducible pBad/araC promoter (I0500) produces temperature-sensitive LacI (LacI ts), which then, upon dimerization, represses the IPTG inducible LacI+ pL promoter (R0011) resulting in a decrease in GFP (E0240)/mRFP (I13507)
- Module 2: In Module 1, insert DNA sequence coding for 434 cI protein instead of the reporters (GFP/mRFP) and then add Prm+ promoter (I12006), which is repressed by 434 cI and add GFP
- Module 3: Take Module 3 and add mRFP (J04450)
