Testing Results
From 2006.igem.org
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| + | == Overview == | ||
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We decided to integrate check-points into our construction plan, in order to test intermediary part functionality. | We decided to integrate check-points into our construction plan, in order to test intermediary part functionality. | ||
<br>There are three basic modules: | <br>There are three basic modules: | ||
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<li>'''Module 3:''' Take Module 3 and add mRFP (J04450) | <li>'''Module 3:''' Take Module 3 and add mRFP (J04450) | ||
</ul> | </ul> | ||
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| + | == Recent Tests == | ||
Revision as of 05:46, 13 October 2006
Overview
We decided to integrate check-points into our construction plan, in order to test intermediary part functionality.
There are three basic modules:
- Module 1: Arabinose inducible pBad/araC promoter (I0500) produces temperature-sensitive LacI (LacI ts), which then, upon dimerization, represses the IPTG inducible LacI+ pL promoter (R0011) resulting in a decrease in GFP (E0240)/mRFP (I13507)
- Module 2: In Module 1, insert DNA sequence coding for 434 cI protein instead of the reporters (GFP/mRFP) and then add Prm+ promoter (I12006), which is repressed by 434 cI and add GFP
- Module 3: Take Module 3 and add mRFP (J04450)
