Testing Results
From 2006.igem.org
(Difference between revisions)
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[[Oct 8, 2006]] | [[Oct 8, 2006]] | ||
<ul> | <ul> | ||
| - | <li>IPTG Induction of mRFP | + | <li>Module 1 (DH5a): IPTG Induction of mRFP |
| - | <li>Arabinose Induction of LacI ts and repression of GFP | + | <li>Module 1 (DH5a): Arabinose Induction of LacI ts and repression of GFP |
</ul> | </ul> | ||
[[Oct 7, 2006]] | [[Oct 7, 2006]] | ||
<ul> | <ul> | ||
| - | <li>IPTG Induction of GFP | + | <li>Module 1 (DH5a-z1): IPTG Induction of GFP |
| - | <li>Arabinose Induction of LacI ts and repression of GFP | + | <li>Module 1 (DH5a): Arabinose Induction of LacI ts and repression of GFP |
</ul> | </ul> | ||
[[Sept 21, 2006]] | [[Sept 21, 2006]] | ||
<ul> | <ul> | ||
| - | <li>Module 1: IPTG Induction of GFP/mRFP | + | <li>Module 1 (DH5a-z1): IPTG Induction of GFP/mRFP |
</ul> | </ul> | ||
[[Sept 16, 2006]] | [[Sept 16, 2006]] | ||
<ul> | <ul> | ||
| - | <li>Module 1: IPTG Induction of GFP | + | <li>Module 1 (DH5a-z1): IPTG Induction of GFP |
</ul> | </ul> | ||
Revision as of 05:52, 13 October 2006
Overview
We decided to integrate check-points into our construction plan, in order to test intermediary part functionality.
There are three basic modules:
- Module 1: Arabinose inducible pBad/araC promoter (I0500) produces temperature-sensitive LacI (LacI ts), which then, upon dimerization, represses the IPTG inducible LacI+ pL promoter (R0011) resulting in a decrease in GFP (E0240)/mRFP (I13507)
- Module 2: In Module 1, insert DNA sequence coding for 434 cI protein instead of the reporters (GFP/mRFP) and then add Prm+ promoter (I12006), which is repressed by 434 cI and add GFP
- Module 3: Take Module 3 and add mRFP (J04450)
Recent Tests
- Module 1 (DH5a): IPTG Induction of mRFP
- Module 1 (DH5a): Arabinose Induction of LacI ts and repression of GFP
- Module 1 (DH5a-z1): IPTG Induction of GFP
- Module 1 (DH5a): Arabinose Induction of LacI ts and repression of GFP
- Module 1 (DH5a-z1): IPTG Induction of GFP/mRFP
- Module 1 (DH5a-z1): IPTG Induction of GFP
